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NCT07279896

Determination of RNA/DNA Damage Associated With Oxidative Stress in Periodontitis Patients

Completed Last updated 12 December 2025
What this trial tests

trial in Periodontitis (Stage 3) in 88 participants. Completed in 15 August 2025.

Timeline
5 May 2025
Primary endpoint
4 August 2025
15 August 2025

Quick facts

Lead sponsorZehra Hasgül
StatusCompleted
Study typeOBSERVATIONAL
Enrollment88
Start date5 May 2025
Primary completion4 August 2025
Estimated completion15 August 2025
Sites1 location across Turkey (Türkiye)

Conditions studied

Sponsor

Zehra Hasgül

Who can join

Adults 18 to 65, any sex, with Periodontitis (Stage 3) or Smoking. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

This study looked at whether a type of gum disease called Stage III periodontitis and cigarette smoking are linked to higher levels of "oxidative stress." Oxidative stress can damage DNA and RNA, which are important parts of our cells. Two markers in saliva-8-OHdG (a sign of DNA damage) and 8-OHG (a sign of RNA damage)-were measured to understand this. Researchers included four groups of adults: smokers with periodontitis, non-smokers with periodontitis, healthy smokers, and healthy non-smokers. All participants had a dental exam, which checked things like gum inflammation, bleeding, and pocket depth. After the exam, an unstimulated saliva sample was collected from each person. The samples were tested in a laboratory using a sensitive method that measures oxidative damage. The study found that people with periodontitis had higher levels of DNA and RNA damage in their saliva than healthy people. The highest levels were seen in smokers with periodontitis. Even healthy smokers showed higher levels of oxidative stress than healthy non-smokers. In general, worse gum disease was linked to higher levels of oxidative damage. These results suggest that saliva tests for 8-OHdG and 8-OHG may help identify gum disease in a simple, non-invasive way. The findings also show that smoking adds to the body's oxidative stress, especially when gum disease is present. The study provides new information about how smoking and gum disease together may affect cell health.

Publications & conference data

No peer-reviewed publications indexed yet for this trial. Completed trials usually publish results within 12-18 months.

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