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NCT03991793: GABEC

Granzyme A in Patients With E. Coli Bacteremic Urinary Tract Infections

Status unknown Last updated 19 June 2019
What this trial tests

trial in Bloodstream Infection in 50 participants. Status unknown.

Timeline
20 June 2019
Primary endpoint
30 May 2020
31 December 2020

Quick facts

Lead sponsorInstituto de Investigación Sanitaria Aragón
StatusStatus unknown
Study typeOBSERVATIONAL
Enrollment50
Start date20 June 2019
Primary completion30 May 2020
Estimated completion31 December 2020

Conditions studied

Sponsor

Instituto de Investigación Sanitaria Aragón — full company profile →

Who can join

18 and older, any sex, with Bloodstream Infection or Sepsis. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Background: Survival in Granzyme A gene (gzmA) knocked-out mice was significantly longer than in wild-type mice in a murine peritonitis model (cecal ligation puncture). Hypothesis: GZM A has a pathogenic role in sepsis in humans and gzmA polymorphisms can help to predict the risk of sepsis among patients with systemic infections (E. coli bacteremic urinary tract infections). Objectives: 1. To assess the correlation between GZM A serum levels and systemic inflammatory response in a human model of infection/sepsis (E. coli bacteremic UTI) 2. To characterize gzmA polymorphisms among patients with E. coli bacteremic UTI 3. To determine GZM A serum kinetics among patients with E. coli bacteremic UTI 4. To characterize E. coli strains causing bacteremic UTI: antimicrobial phenotype and virulence factors ("virulome"). Methods: * Design and setting: Prospective nested case-control study * Study population: consecutive adult patients with bacteremic urinary tract infections (UTIs) caused by E. coli * Exclusion criteria: Patients with conditions that significantly compromise immune status or patients exposed to urologic procedures * Estimated sample size: 50 patients with a sepsis/ non sepsis 1:1 ratio. Septic and non septic patients will be matched on gender, age (+/- 10 years), comorbidity (Charlson score +/-1), time symptom onset to blood culture (+/- 24h) * Measurements: GZM A serum levels will be determined on day 0, day 2-3, day 30. GZM A kinetics, gzmA polymorphisms (whole exome sequencing).Whole genome sequencing of E. coli isolates retrieved from blood cultures will be performed. * Analysis: Association between GZM A levels and gzmA polymorphisms and sepsis will be analyzed adjusting for patient, infection and microorganism-related factors (multivariate analysis).

Publications & conference data

No peer-reviewed publications indexed yet for this trial.

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Other recruiting trials for Bloodstream Infection

Currently open trials in the same condition.

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Data sources for this page

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