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NCT02086110

Effect of Milk Oligosaccharides and Bifidobacteria on the Intestinal Microflora of Children With Autism

Completed NA Results posted Last updated 1 August 2019
What this trial tests

NA trial testing Synbiotic in Autism in 11 participants. Completed in 16 November 2015.

Timeline
6 June 2014
Primary endpoint
16 November 2015
16 November 2015

Quick facts

Lead sponsorUniversity of California, Davis
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationrandomized
Designcrossover
Maskingtriple
Primary purposebasic science
Enrollment11
Start date6 June 2014
Primary completion16 November 2015
Estimated completion16 November 2015
Sites1 location across United States

Drugs / interventions tested

Conditions studied

Sponsor

University of California, Davis

Who can join

Adults 2 to 11, any sex, with Autism. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Stool Microbiota Composition Change During Prebiotic Only Treatment Primary · Five weeks

The stool microbiome composition will be analyzed through next generation sequencing and quantitative polymerase chain reaction. Overall bacterial composition change is reported here, as change in enterotype from before prebiotic only treatment to after prebiotic treatment, for all subjects. Enterotypes were organized into four categories for analysis: a community high in Prevotella ("Prevotella"), a community high in Bifidobacterium ("Bifidobacterium"), a community high in Bacteroides ("Bacteroides"), and a mixed community ("Mixed") that did not fall into one of the other three enterotypes bu

GroupValue95% CI
Prebiotic Only First, Then Synbiotic2
Synbiotic First, Then Prebiotic Only3
Prebiotic Only First, Then Synbiotic1
Synbiotic First, Then Prebiotic Only0
Prebiotic Only First, Then Synbiotic1
Synbiotic First, Then Prebiotic Only0
Prebiotic Only First, Then Synbiotic0
Synbiotic First, Then Prebiotic Only1
Stool Microbiota Composition Change During Synbiotic Treatment Primary · Five weeks

The stool microbiome composition will be analyzed through next generation sequencing and quantitative polymerase chain reaction. Overall bacterial composition change is reported here, as change in enterotype from before synbiotic treatment to after synbiotic treatment, for all subjects. Enterotypes were organized into four categories for analysis: a community high in Prevotella ("Prevotella"), a community high in Bifidobacterium ("Bifidobacterium"), a community high in Bacteroides ("Bacteroides"), and a mixed community ("Mixed") that did not fall into one of the other three enterotypes but ins

GroupValue95% CI
Prebiotic Only First, Then Synbiotic4
Synbiotic First, Then Prebiotic Only2
Prebiotic Only First, Then Synbiotic0
Synbiotic First, Then Prebiotic Only1
Prebiotic Only First, Then Synbiotic0
Synbiotic First, Then Prebiotic Only1
Serum Immune Profile Change During Prebiotic Only Treatment Secondary · Five weeks

Luminex technology will be used to determine a serum immune profile of each participants in response to the study supplement. This profile included assessment of change in percentage of stimulated CD4+ T cells producing intracellular IL-13 before and after Synbiotic treatment ("Post-Synbiotic % IL-13"), and assessment of change in percentage of stimulated CD8+ T cells producing TNF-alpha before and after Prebiotic Only treatment ("Post-Prebiotic % TNF-alpha"). Results are reported here for all subjects regardless of treatment order assignment. Decrease in inflammatory cytokines IL-13 and TNF-a

Post-Synbiotic % IL-13
GroupValue95% CI
All Subjects-0.8-2.073 – -0.524
Post-Prebiotic % TNF-alpha
GroupValue95% CI
All Subjects-7.9-14.375 – -1.425

Adverse events — posted to ClinicalTrials.gov

Time frame: Adverse event data was collected from time of consent through end-of-treatment (12 weeks total).. Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Prebiotic Only Arm
Serious: 0/10 (0%)
Deaths: 0/10
Synbiotic Arm
Serious: 0/10 (0%)
Deaths: 0/10
Other adverse events (10 terms — click to expand)

ReactionSystemPrebiotic Only ArmSynbiotic Arm
Pain associated with bowel movementsGastrointestinal disorders
Increased gassinessGastrointestinal disorders
DiarrheaGastrointestinal disorders
ConstipationGastrointestinal disorders
BloatingGastrointestinal disorders
Weight gainMetabolism and nutrition disorders
StomachacheGastrointestinal disorders
Sleep disturbancesPsychiatric disorders
LethargyPsychiatric disorders
HivesSkin and subcutaneous tissue disorders

Data from ClinicalTrials.gov NCT02086110 adverse events section.

Sponsor's own description

The purpose of this study is to determine the tolerability of dietary supplements and if these supplements can promote a healthy bacterial environment in the intestines of children with autism spectrum disorders and gastrointestinal complaints.

Publications & conference data

7 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Pilot study of probiotic/colostrum supplementation on gut function in children with autism and gastrointestinal symptoms.
    Sanctuary MR, Kain JN, Chen SY, Kalanetra K, et al · · 2019 · cited 148× · PMID 30625189 · DOI 10.1371/journal.pone.0210064
  2. <i>Bifidobacterium longum</i> Subspecies <i>infantis</i> (<i>B. infantis</i>) in Pediatric Nutrition: Current State of Knowledge.
    Chichlowski M, Shah N, Wampler JL, Wu SS, et al · · 2020 · cited 84× · PMID 32481558 · DOI 10.3390/nu12061581
  3. Mechanisms and Clinical Implications of Human Gut Microbiota-Drug Interactions in the Precision Medicine Era.
    Wang S, Ju D, Zeng X. · · 2024 · cited 25× · PMID 38255298 · DOI 10.3390/biomedicines12010194
  4. CD8 T cells drive anorexia, dysbiosis, and blooms of a commensal with immunosuppressive potential after viral infection.
    Labarta-Bajo L, Gramalla-Schmitz A, Gerner RR, Kazane KR, et al · · 2020 · cited 23× · PMID 32958643 · DOI 10.1073/pnas.2003656117
  5. Does the Gut Microbial Metabolome Really Matter? The Connection between GUT Metabolome and Neurological Disorders.
    Marć MA, Jastrząb R, Mytych J. · · 2022 · cited 18× · PMID 36235622 · DOI 10.3390/nu14193967
  6. Analysis of research on the effectiveness of using probiotics for children with autism spectrum disorders, in order to reduce the core and accompanying autism symptoms. Review of randomized clinical trials.
    Brzóska-Konkol E, Remberk B, Papasz-Siemienuk A. · · 2022 · cited 6× · PMID 37082415 · DOI 10.5114/ppn.2022.115244
  7. The endocannabinoidome-gut microbiome-brain axis as a novel therapeutic target for autism spectrum disorder.
    Campanale A, Siniscalco D, Di Marzo V. · · 2025 · cited 2× · PMID 40605060 · DOI 10.1186/s12929-025-01145-7

Verify or expand the search:

Other trials of Synbiotic

Trials testing the same drug.

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Currently open trials in the same condition.

Other University of California, Davis trials

Trials by the same sponsor.

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