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Neostigmine and Glycopyrrolate

James J. Peters Veterans Affairs Medical Center · FDA-approved active Small molecule

Neostigmine inhibits acetylcholinesterase to increase acetylcholine levels, while glycopyrrolate blocks muscarinic receptors to counteract cholinergic side effects.

Neostigmine inhibits acetylcholinesterase to increase acetylcholine levels, while glycopyrrolate blocks muscarinic receptors to counteract cholinergic side effects. Used for Myasthenia gravis, Reversal of neuromuscular blockade (anesthesia).

At a glance

Generic nameNeostigmine and Glycopyrrolate
Also known asGlycostigmin.
SponsorJames J. Peters Veterans Affairs Medical Center
Drug classCholinesterase inhibitor with anticholinergic agent
TargetAcetylcholinesterase (neostigmine); Muscarinic acetylcholine receptors (glycopyrrolate)
ModalitySmall molecule
Therapeutic areaNeurology
PhaseFDA-approved

Mechanism of action

Neostigmine is a cholinesterase inhibitor that prevents the breakdown of acetylcholine, thereby enhancing neuromuscular transmission and parasympathetic activity. Glycopyrrolate is an anticholinergic agent added to this combination to block unwanted muscarinic effects (such as excessive salivation, bronchospasm, and bradycardia) that would otherwise result from neostigmine monotherapy. Together, they provide the therapeutic benefits of cholinergic enhancement while mitigating parasympathomimetic adverse effects.

Approved indications

Common side effects

Key clinical trials

Primary sources

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SourceUsed for
ClinicalTrials.govTrial enrolment, design, endpoints, results

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