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Leucovorin calcium/Levoleucovorin

Janssen Research & Development, LLC · Phase 3 active Small molecule

Leucovorin calcium and levoleucovorin are reduced folate cofactors that enhance the efficacy of antimetabolite chemotherapy agents by providing essential one-carbon units for nucleotide synthesis.

Leucovorin calcium and levoleucovorin are reduced folate cofactors that enhance the efficacy of antimetabolite chemotherapy agents by providing essential one-carbon units for nucleotide synthesis. Used for Metastatic colorectal cancer (in combination with fluorouracil), High-dose methotrexate toxicity rescue, Fluorouracil-based chemotherapy potentiation.

At a glance

Generic nameLeucovorin calcium/Levoleucovorin
Also known asFolinic acid
SponsorJanssen Research & Development, LLC
Drug classReduced folate cofactor; chemotherapy adjuvant
TargetThymidylate synthase (indirect); folate metabolism pathway
ModalitySmall molecule
Therapeutic areaOncology
PhasePhase 3

Mechanism of action

Leucovorin (folinic acid) and its active L-isomer levoleucovorin bypass the dihydrofolate reductase step and directly provide 5,10-methylenetetrahydrofolate, a critical cofactor for thymidylate synthase and other folate-dependent enzymes. This potentiates the cytotoxic effects of fluorouracil and methotrexate by stabilizing their target enzyme complexes and enhancing DNA synthesis inhibition. Levoleucovorin is the pharmacologically active form, requiring lower doses than the racemic leucovorin calcium.

Approved indications

Common side effects

Key clinical trials

Primary sources

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SourceUsed for
ClinicalTrials.govTrial enrolment, design, endpoints, results

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