Last reviewed · How we verify
Relationship Between mexB Efflux Pump Gene Expression, Efflux Pump Activity and Biofilm Formation in Multidrug Resistant Clinical Isolates of Pseudomonas Aeruginosa
1. Detection of expression level of mexB efflux pump gene in multidrug resistant clinical isolates of Pseudomonas aeruginosa by real time PCR(QRT-PCR). 2. Phenotypic detection of efflux pump activity in multidrug resistant clinical isolates of Pseudomonas aeruginosa. 3. Phenotypic detection of biofilm formation and its degree (strong-moderate-weak) in multidrug resistant clinical isolates of Pseudomonas aeruginosa. 4. Relationship between expression level of mexB efflux pump gene and degree of biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa.
Details
| Lead sponsor | Assiut University |
|---|---|
| Status | NOT_YET_RECRUITING |
| Enrolment | 80 |
| Start date | 2026-04 |
| Completion | 2028-01 |
Conditions
- Pseudomonas Aeroginosa
Interventions
- Real time PCR
Primary outcomes
- Detection of mexB efflux pump gene expression level in multidrug resistant clinical isolates of Pseudomonas aeruginosa — one year
molecular detection of mexB efflux pump gene expression level by real time PCR (QRT-PCR)