NCT07241689

Effects of Protein Supplementation on 4 Weeks of Alternate-day Fasting on Fasting Blood Glucose, Resting Blood Pressure, and Body Composition in Young Men

Completed NA Last updated 21 November 2025

What this trial tests

NA trial testing protein supplement in Obesity & Overweight in 26 participants. Completed in 23 March 2018.

Timeline

17 January 2018

Primary endpoint
23 March 2018

23 March 2018

Quick facts

Lead sponsor	Nanyang Technological University
Phase	NA
Status	Completed
Study type	INTERVENTIONAL
Allocation	randomized
Design	parallel
Masking	none
Primary purpose	treatment
Enrollment	26
Start date	17 January 2018
Primary completion	23 March 2018
Estimated completion	23 March 2018
Sites	1 location across Singapore

Drugs / interventions tested

• protein supplement — full drug profile →
• No supplementation — full drug profile →

Conditions studied

• Obesity & Overweight — all drugs for Obesity & Overweight →

Sponsor

Nanyang Technological University

Who can join

Adults 21 to 35, male only, with Obesity & Overweight. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

The objectives of this study are to examine the effects of protein supplementation on 4 weeks of alternate-day fasting (ADF) on fasting blood glucose, resting blood pressure, and body composition in young Asian men with unhealthy body mass index ≥ 23 kg/m2.

To achieve the objective, 40 young men aged 21 to 35 years old will be recruited to participate in this study and complete a 4-week ADF programme. The fasting programme starts at 12 midnight and alternates between fasting and feeding days. Each fasting or feeding day is 24-hour in duration. On fasting days, no food and beverages are allowed except for ad libitum intake of plain water and/or other zero-calorie beverages, and a small meal between 400-600 kcal. In this study, participants would be randomly assigned to either the control or protein supplementation group (20 in each group). The control group will consume a small meal of their choice as long as it is between 400-600 kcal while the protein supplementation group will consume a small meal between 400-600 kcal that will include a protein supplement that would be provided (125 kcal, 25 g protein) and with the remainder of the meal of their choice. For both groups in this study, the small meal is to be consumed during 12 pm to 2 pm for standardisation to ensure consistency in total duration of fasting and feeding. The programme will end after 4 weeks (28 days) of continuous alternate-day fasting.

Fasting blood glucose, resting blood pressure and heart rate, body mass, height, and body composition will be measured before and after 4-week of ADF. In addition, these measurements, except body composition, will be repeated at the end of each week of ADF.

The research hypothesis are: 1) 4 weeks of ADF will reduce body mass via both fat and muscle loss, and improve health indices as measured by fasting blood glucose and resting blood pressure in young individuals with BMI greater than the desired healthy range, and 2) protein supplementation during fasting days will reduce muscle loss during loss of body mass.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

1. Effects of Four Weeks of Alternate-Day Fasting with or Without Protein Supplementation-A Randomized Controlled Trial.
   Pang BWJ, Yang Y, Rashiqah N, Huang CB, et al · · 2025 · PMID 41373982 · DOI 10.3390/nu17233691