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Association Between Age-related Macular Degeneration and Cognitive Impairment Based on Exosome Proteomics
Aim: Preliminary serum exosomal proteomics of patients with dry age-related macular degeneration (AMD) and mild cognitive impairment (MCI) were analyzed using proteomics technology. Methods: Peripheral serum samples were collected from patients with AMD, MCI, comorbid conditions, and the control group. Exosomes were isolated from these samples and analyzed using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Differentially expressed proteins (DEPs) associated with the two diseases were identified based on fold change and p-value. DEPs were analyzed by Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) network analysis. Western blot and enzyme-linked immunosorbent assay (ELISA) were used to validate the DEPs of interest.
Details
| Lead sponsor | Shanghai 10th People's Hospital |
|---|---|
| Status | COMPLETED |
| Enrolment | 120 |
| Start date | Mon Apr 01 2019 00:00:00 GMT+0000 (Coordinated Universal Time) |
| Completion | Mon Jun 03 2024 00:00:00 GMT+0000 (Coordinated Universal Time) |
Conditions
- Age Related Macular Degeneration
- Mild Cognitive Impairment (MCI)
- Alzheimer's Disease (AD)
Countries
China