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NCT06684860

RUT vs. Molecular Testing for H. Pylori

Recruiting now Last updated 29 April 2025
What this trial tests

trial in H.Pylori Infection in 100 participants. Currently enrolling.

Timeline
1 November 2024
Primary endpoint
31 December 2025
31 December 2025

Quick facts

Lead sponsorSoonchunhyang University Hospital
StatusRecruiting now
Study typeOBSERVATIONAL
Enrollment100
Start date1 November 2024
Primary completion31 December 2025
Estimated completion31 December 2025
Sites1 location across South Korea

Conditions studied

Sponsor

Soonchunhyang University Hospital

Who can join

Adults 20 to 80, any sex, with H.Pylori Infection. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

The rapid urease test (RUT) is frequently performed for diagnosis of Helicobacter pylori infection in clinical practice. The RUT is a simple and inexpensive method that utilizes a commercial kit containing urea and a pH indicator such as phenol red. In the presence of H. pylori urease, urea is hydrolyzed to ammonia, which raises the pH of the medium, and changes the color of the kit from yellow to magenta or pink. The sensitivity and specificity of RUT were reported to range between 80-100% and 97-99%, respectively. When endoscopy is performed, RUT is known as the first-line method used for H. pylori diagnosis. However, RUT is an indirect test method, and has the disadvantage of causing false positivity due to other urease-producing bacteria. To date, gold standards for H. pylori diagnosis are culture and histological examination Because H. pylori is a rather fastidious and slow-growing bacterium, antimicrobial susceptibility testing using culture methods is time-consuming (10-14 days) and requires specific expertise. Recently, dual priming oligonucleotide (DPO)-based multiplex polymerase chain reaction (PCR) was developed to diagnose H. pylori infection. Moreover, molecular testing can identify point mutations related to clarithromycin resistance. It can be performed in a standard thermocycler and takes only 4 h to complete. The concordance rate between conventional clarithromycin susceptibility testing and DPO-PCR was excellent up to 95%. However, there was no comparison study between RUT and molecular testing for H. pylori diagnosis.

Publications & conference data

No peer-reviewed publications indexed yet for this trial.

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Other recruiting trials for H.Pylori Infection

Currently open trials in the same condition.

Other Soonchunhyang University Hospital trials

Trials by the same sponsor.

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Data sources for this page

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