NCT06596395: CAF+BJ

Effect of Acute Caffeine and Beetroot Juice Intake on Strength, Power, and Muscular Endurance

Completed NA Last updated 19 September 2024

What this trial tests

NA trial testing Acute caffeine and beetroot juice in Dietary Supplements in 14 participants. Completed in 1 May 2024.

Timeline

1 September 2023

Primary endpoint
30 January 2024

1 May 2024

Quick facts

Lead sponsor	University of Alcala
Phase	NA
Status	Completed
Study type	INTERVENTIONAL
Allocation	randomized
Design	crossover
Masking	triple
Primary purpose	other
Enrollment	14
Start date	1 September 2023
Primary completion	30 January 2024
Estimated completion	1 May 2024
Sites	1 location across Spain

Drugs / interventions tested

• Acute caffeine and beetroot juice
• Acute caffeine
• Acute beetroot juice
• Placebo

Conditions studied

• Dietary Supplements — all drugs for Dietary Supplements →
• Caffeine — all drugs for Caffeine →
• Nitrates — all drugs for Nitrates →
• Sports Performance — all drugs for Sports Performance →

Sponsor

University of Alcala

Who can join

Adults 18 to 35, male only, with Dietary Supplements or Caffeine. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Introduction: Several studies have evaluated and confirmed the ergogenic effect of acute caffeine and beetroot juice intake on sports performance. However, there are few studies focused on the production of strength, power, and muscle endurance, particularly when both supplements are ingested together.

Objectives: Evaluate the acute effect of combined and isolated intake of caffeine and beetroot juice on the production of strength, power, and muscle endurance in young adults, analyzing the effect of circadian rhythms (morning vs. afternoon) on the exercise performed (bench press vs. squat).

Methods: A total of 12 healthy and physically active participants will be recruited for the study. Through a double-blind, crossover, randomized, and controlled design, participants will ingest: a) Caffeine (3 mg/kg body mass); b) Beetroot juice (70 ml, 6.5 mmol of nitrates); c) Caffeine + Beetroot juice; d) Placebo (maltodextrin, 3 mg/kg). The supplements will be ingested 120 minutes (beetroot juice or placebo) and 60 minutes before (caffeine or placebo), the latter dissolved in 200 ml of water. After the ingestion period, strength, power, and muscle endurance will be analyzed with 1RM tests, strength and power tests at 25%, 50%, 75%, and 90% 1RM, and muscle endurance tests at 65% 1RM, in all cases for squat and bench press exercises. Additionally, dynamometry (manual and the isometric mid-thigh pull test) and vertical jump (squat jump and countermovement jump) will be analyzed. The four experimental conditions will be conducted in the morning (8:00 am to 10:00 am) and in the afternoon (4:30 pm to 6:30 pm).

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

1. Single and combined effect of beetroot juice and caffeine intake on muscular strength, power and endurance performance in resistance-trained males.
   Montalvo-Alonso JJ, Del Val-Manzano M, Ferragut C, Valadés D, et al · · 2025 · cited 2× · PMID 40369047 · DOI 10.1038/s41598-025-02021-y