NCT06095349: FORST3

Effect of Sulfur Thermal Water on Circulating H2S Levels and Markers of Bone Metabolism in Osteopenic Subjects.

Completed NA Last updated 19 January 2024

What this trial tests

NA trial testing inhalatory treatment with sulfureous aerosol in Hydrogen Sulfide in 50 participants. Completed in 30 November 2023.

Timeline

1 October 2019

Primary endpoint
30 November 2023

30 November 2023

Quick facts

Lead sponsor	Istituto Ortopedico Rizzoli
Phase	NA
Status	Completed
Study type	INTERVENTIONAL
Allocation	na
Design	single group
Masking	none
Primary purpose	prevention
Enrollment	50
Start date	1 October 2019
Primary completion	30 November 2023
Estimated completion	30 November 2023
Sites	1 location across Italy

Drugs / interventions tested

• inhalatory treatment with sulfureous aerosol

Conditions studied

• Hydrogen Sulfide — all drugs for Hydrogen Sulfide →
• Osteoporosis — all drugs for Osteoporosis →

Sponsor

Istituto Ortopedico Rizzoli — full company profile →

Who can join

Adults 50 to 60, female only, with Hydrogen Sulfide or Osteoporosis. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

The aim of the study is to investigate whether inhalation treatment with high-sulfide waters from some spas (referred to as "thermal waters"), performed for 12 consecutive days, is able to change circulating H2S levels and alter bone metabolism. Identifying a clear correlation between circulating levels of H2S and sulfur water administration, as well as a potential anabolic effect on bone, would set the stage for new studies that could have important clinical implications for promoting health and preventing osteoporosis

Publications & conference data

2 peer-reviewed publications reference this trial (live from Europe PMC):

1. Reactive Sulfur Species and Protein Persulfidation: An Emerging Redox Axis in Human Health and Disease.
   Andrés CMC, Lobo F, Lastra JMP, Munguira EB, et al · · 2025 · cited 7× · PMID 41020887 · DOI 10.3390/cimb47090765
2. Changes in Oxidative Stress, Inflammatory and Bone Metabolism Biomarkers Following Sulfurous Water Inhalation in Osteopenic Women.
   Gambari L, Amore E, Roseti L, Carpentieri S, et al · · 2026 · PMID 41977347 · DOI 10.3390/ijms27073163