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NCT06084377

Isolation of Cell Free Fetal DNA From Spent Culture Medium and Its Potential Role for Preimplantation Genetic Diagnosis (PGD).

Completed Last updated 18 October 2023
What this trial tests

trial testing Preimplantation Genetic Screening in Females Undergoing PGD Technology for Medical Conditions in 27 participants. Completed in 23 September 2023.

Timeline
18 May 2020
Primary endpoint
5 April 2023
23 September 2023

Quick facts

Lead sponsorDamascus University
StatusCompleted
Study typeOBSERVATIONAL
Enrollment27
Start date18 May 2020
Primary completion5 April 2023
Estimated completion23 September 2023
Sites1 location across Syria

Drugs / interventions tested

Conditions studied

Sponsor

Damascus University

Who can join

Adults 22 to 44, female only, with Females Undergoing PGD Technology for Medical Conditions. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Preimplantation genetic testing for chromosomal screening is, at present, the most reliable method to assess the genetic status of preimplantation embryos. DNA isolated and amplified from trophectoderm (TE) biopsiesis the method currently used for preimplantation genetic testing, but it has significant limitations. There is increasing evidence for a ''true'' noninvasive approach consisting of the analysis of cell-free DNA (cfDNA) released by the embryo into the spent blastocyst medium (SBM) during the late stages of preimplantation development. This study is to assess whether Noninvasive prenatal genetic diagnosis using cell-free fetal DNA in spent culture medium will substitute for trophectoderm biopsy for heritable disorders screening and if it represents the total fetal genomic DNA. A total of 27 spent blastocyst media (SBM) from women undergoing IVF will be enrolled with agesless than44 years, whose indications for preimplantation genetic diagnosis for Euploid/Aneuploid embryo determination, were advanced maternal age, recurrent miscarriage, or recurrent implantation failure.We examined the presence of the cell free DNA in spent culture media and whether this DNA is reliable and can be representative of the chromosomal constitution of a blastocyst. So we can affirm that Noninvasive prenatal determination of fetal sex using cell-free fetal DNA (used here as a marker) provides an alternative to invasive techniques for some heritable disorders.

Publications & conference data

No peer-reviewed publications indexed yet for this trial. Completed trials usually publish results within 12-18 months.

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Other trials of Preimplantation Genetic Screening

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