NCT05995158

Metagenomic Analysis of the Link Between Periodontitis and Smoking: Case-control Study

Completed Last updated 16 August 2023

What this trial tests

trial testing Metagenomic testing of the subgingival plaque samples in Condition, Chronic Periodontitis in 60 participants. Completed in 30 January 2023.

Timeline

1 January 2022

Primary endpoint
30 November 2022

30 January 2023

Quick facts

Lead sponsor	King Abdulaziz University
Status	Completed
Study type	OBSERVATIONAL
Enrollment	60
Start date	1 January 2022
Primary completion	30 November 2022
Estimated completion	30 January 2023
Sites	1 location across Saudi Arabia

Drugs / interventions tested

• Metagenomic testing of the subgingival plaque samples

Conditions studied

• Condition, Chronic Periodontitis — all drugs for Condition, Chronic Periodontitis →

Sponsor

King Abdulaziz University

Who can join

18 and older, any sex, with Condition, Chronic Periodontitis. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Periodontitis is a widely prevalent disease worldwide that has serious public health consequences. Its prognosis includes tooth loss and edentulism, a condition that negatively affects chewing causing functional disability; and esthetics causing social impairment. Consequently, periodontitis may end up causing marked impairment of the quality of life of the affected patients, impairment of general health and increasing the dental care costs significantly.

Dysbiotic changes in the oral microbiome arise after some microbial species are enriched by primary products resulting from tissue breakdown due to gingivitis. It then triggers the host cells to produce proteinases that mediate loss of marginal periodontal ligaments, apical migration of the junctional epithelium and apical spread of bacterial biofilm.

However, the dysbiotic changes may be more likely to occur in some patients rather than others due to certain risk factors including smoking and immuneinflammatory responses. Thus, the severity of periodontal disease in these patients would be higher. Tobacco smoking is no longer considered to be a habit, but a dependence to nicotine and a chronic relapsing medical disorder. Among its detrimental effects on general health, tobacco smoking increases the risk of periodontitis by 2 to 5 folds. This takes place by increasing the dysbiotic changes in the oral microbiome and so, increasing the severity and extent of the periodontal disease at a younger age. Therefore, smoking has been considered as a modifying factor of periodontitis that should be considered upon periodontitis case grading definition.

Therefore, this research aims to identify the difference in dysbiosis between the three categories of periodontitis, trying to understand the cause of the resistance of each category to treatment compared to the milder category.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

1. Effect of Smoking on Subgingival Microbiome in Chronic Periodontitis: A 16S rRNA Sequencing Study.
   Alblowi JA. · · 2025 · PMID 41590134 · DOI 10.3390/dj14010010