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NCT05836012

Safety and Immunogenicity of HIL-214 With Routine Pediatric Vaccines

Completed Phase 2, PHASE3 Results posted Last updated 12 March 2025
What this trial tests

Phase 2, PHASE3 trial testing HIL-214 in Gastroenteritis in 329 participants. Completed in 8 July 2024.

Timeline
10 June 2023
Primary endpoint
19 January 2024
8 July 2024

Quick facts

Lead sponsorHilleVax
PhasePhase 2, PHASE3
StatusCompleted
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingquadruple
Primary purposeprevention
Enrollment329
Start date10 June 2023
Primary completion19 January 2024
Estimated completion8 July 2024
Sites12 locations across Panama, Puerto Rico, United States

Drugs / interventions tested

Conditions studied

Sponsor

HilleVax — full company profile →

Who can join

Adults 2 Months to 2 Months, any sex, with Gastroenteritis. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Immune Response to the Licensed Pediatric DTaP-Hib-IPV-HepB Vaccine Co-administered With a 2-dose Regimen of HIL-214 at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Primary · 28 days post-dose 2

Evaluate the immune response to the licensed pediatric DTaP-Hib-IPV-HepB vaccine co-administered with a 2-dose regimen of HIL-214 at 4 and 6 months of age, compared to that of the routine pediatric vaccine co-administered with placebo. Due to differing local availability of licensed DTaP-Hib-IPV-HepB vaccine, data are presented by country (Panama and USA). Outcome measures: * Binary (yes/no) variable indicating anti-DT immunoglobulin G (IgG) concentration ≥0.1 IU/mL. * Binary variable indicating anti-TT IgG concentration ≥0.1 IU/mL. * Anti-pertussis \[FHA\], \[PRN\] and \[PTX\]) IgG concentr

Anti-diphtheria toxoid IgG concentration ≥0.1 IU/mL.
GroupValue95% CI
Placebo (Panama)99.3
HIL-214 (Panama)100
Placebo (USA)100
HIL-214 (USA)100
Anti-tetanus toxoid IgG concentration ≥0.1 IU/mL
GroupValue95% CI
Placebo (Panama)100
HIL-214 (Panama)100
Placebo (USA)100
HIL-214 (USA)100
Anti-poliovirus type 1 neutralizing antibody titers ≥3 log2
GroupValue95% CI
Placebo (Panama)100
HIL-214 (Panama)100
Placebo (USA)100
HIL-214 (USA)83.3
Anti-poliovirus type 2 neutralizing antibody titers ≥3 log2
GroupValue95% CI
Placebo (Panama)100
HIL-214 (Panama)100
Placebo (USA)100
HIL-214 (USA)100
Anti-poliovirus type 3 neutralizing antibody titers ≥3 log2
GroupValue95% CI
Placebo (Panama)99.3
HIL-214 (Panama)99.2
Placebo (USA)100
HIL-214 (USA)100
Anti-PRP IgG concentration ≥ 0.15 ug/mL
GroupValue95% CI
Placebo (Panama)100
HIL-214 (Panama)100
Placebo (USA)100
HIL-214 (USA)100
Anti-HBsAg IgG concentration ≥10 mIU/mL
GroupValue95% CI
Placebo (Panama)100
HIL-214 (Panama)100
Placebo (USA)100
HIL-214 (USA)100
Immune Response to the Licensed Pediatric DTaP-Hib-IPV-HepB Vaccine Co-administered With a 2-dose Regimen of HIL-214 at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Primary · 28 days post-dose 2

Evaluate the immune response to the licensed pediatric DTaP-Hib-IPV-HepB vaccine co-administered with a 2-dose regimen of HIL-214 at 4 and 6 months of age, compared to that of the routine pediatric vaccine co-administered with placebo. Due to differing local availability of licensed DTaP-Hib-IPV-HepB vaccine, data are presented by country (Panama and USA). Outcome measures: Geometric mean anti-FHA, anti-PRN, and anti-PTX concentrations

Anti-pertussis FHA concentration
GroupValue95% CI
Placebo (Panama)83.958± 1.8338
HIL-214 (Panama)78.726± 1.8566
Placebo (USA)32.515± 2.9346
HIL-214 (USA)26.358± 2.7499
Anti-pertactin (PRN) concentration
GroupValue95% CI
Placebo (Panama)92.605± 2.1811
HIL-214 (Panama)84.617± 2.2496
Placebo (USA)61.222± 2.6526
HIL-214 (USA)43.417± 2.7743
Anti-pertussis toxin (PTX) concentration
GroupValue95% CI
Placebo (Panama)48.536± 1.8552
HIL-214 (Panama)48.906± 2.0150
Placebo (USA)80.713± 2.2249
HIL-214 (USA)43.066± 2.5144
Immune Response to the Licensed Pediatric Pneumococcal 13 Valent (PCV13) Vaccine Co-administered With a 2-dose Regimen of HIL-214 at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Primary · 28 days post-dose 2

The outcome was assessed using measurements of immune response to the concomitant anti-pneumococcal capsular polysaccharide IgG \[serotypes 1, 3, 4, 5, 6A, 6B, 7F, 9V, 14, 19A, 19F, 18C, and 23F\]) at 28 days post-dose. Geometric mean concentrations are presented.

Anti-pneumococcal IgG concentration for serotype 1
GroupValue95% CI
Placebo5.255± 1.9827
HIL-2145.802± 2.2008
Anti-pneumococcal IgG concentration for serotype 3
GroupValue95% CI
Placebo0.777± 2.3728
HIL-2140.747± 2.2117
Anti-pneumococcal IgG concentration for serotype 4
GroupValue95% CI
Placebo2.958± 1.8468
HIL-2142.773± 2.0253
Anti-pneumococcal IgG concentration for serotype 5
GroupValue95% CI
Placebo2.378± 1.9223
HIL-2142.429± 1.9277
Anti-pneumococcal IgG concentration for serotype 6A
GroupValue95% CI
Placebo7.660± 2.0384
HIL-2147.414± 2.2194
Anti-pneumococcal IgG concentration for serotype 6B
GroupValue95% CI
Placebo6.419± 2.3854
HIL-2145.459± 2.7604
Anti-pneumococcal IgG concentration for serotype 7F
GroupValue95% CI
Placebo5.091± 1.6726
HIL-2145.063± 1.7727
Anti-pneumococcal IgG concentration for serotype 9V
GroupValue95% CI
Placebo3.881± 1.9259
HIL-2143.568± 2.1651
Immune Response to the Licensed Pediatric Rotavirus Vaccine (RV1) Vaccine Co-administered With a 2-dose Regimen of HIL-214 at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Primary · 28 days post-dose 2

This outcome was assessed using measurements of immune response to the concomitant RV1 vaccine (anti-RV1 IgA). Geometric mean concentrations at 28 days post-dose 2 are reported.

GroupValue95% CI
Placebo67.772± 4.0633
HIL-21487.694± 4.2618
Immunogenicity of a 2-dose Regimen of HIL-214 Co-administered With Routine Pediatric Vaccines at 4 and 6 Months of Age. Secondary · Pre-dose 1 and 28 days post-dose 2

Anti-norovirus (GI.1 and GII.4c) HBGA-blocking antibodies were measured at prior to dose 1 (\~4 months of age) and 28 days post-dose 2 (\~6 months of age). Seroresponse was defined as a fold increase from baseline greater than or equal to 4. Seroresponse rates and 95% confidence intervals (CIs) are presented. Data are stratified by country (Panama and USA).

Anti-GI.1 seroresponse by HBGA-blocking assay at 28 days post-dose 2
GroupValue95% CI
Placebo (Panama)4.11.3 – 9.2
HIL-214 (Panama)97.592.7 – 99.5
Placebo (USA)00.0 – 45.9
HIL-214 (USA)10059.0 – 100
Anti-GII.4c seroresponse by HBGA-blocking assay at 28 days post-dose 2
GroupValue95% CI
Placebo (Panama)1.60.2 – 5.8
HIL-214 (Panama)51.341.9 – 60.5
Placebo (USA)00.0 – 52.5
HIL-214 (USA)16.70.4 – 64.1
Anti-GI.1 and GII.4c seroresponse by HBGA-blocking assay at 28 days post-dose 2
GroupValue95% CI
Placebo (Panama)0.80.0 – 4.5
HIL-214 (Panama)53.043.5 – 62.4
Placebo (USA)00.0 – 52.2
HIL-214 (USA)16.70.4 – 64.1
Evaluate the Safety Profile of a 2-dose Regimen of HIL-214 Co-administered With Routine Pediatric Vaccines at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Secondary · Day 1 to Day 7 post-dose 1 and Day 1 to Day 7 post-dose 2

Percentage of participants with solicited local (injection site) reactions within 7 days of any vaccine administration. Assessed reactions included pain, redness, induration, and swelling.

GroupValue95% CI
Placebo38.4
HIL-21441.2
Evaluate the Safety Profile of a 2-dose Regimen of HIL-214 Co-administered With Routine Pediatric Vaccines at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Secondary · Day 1 to Day 7 post-dose 1 and Day 1 to Day 7 post-dose 2

Percentage of participants with solicited systemic adverse events (AEs) within 7 days of any vaccine administration. Assessed AEs included drowsiness, irritability/fussiness, loss of appetite, fever, vomiting, and diarrhea.

GroupValue95% CI
Placebo54.9
HIL-21446.1
Evaluate the Safety Profile of a 2-dose Regimen of HIL-214 Co-administered With Routine Pediatric Vaccines at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo. Secondary · Day 1 to 28 days post-dose 1 (vaccine discontinuation); Day 1 to 12 months post-dose 1

Percentage of participants with adverse events (AEs) leading to vaccine discontinuation or trial withdrawal.

AE leading to vaccine discontinuation
GroupValue95% CI
Placebo0
HIL-2140
AE leading to trial withdrawal
GroupValue95% CI
Placebo0
HIL-2140
Evaluate the Safety Profile of a 2-dose Regimen of HIL-214 Co-administered With Routine Pediatric Vaccines at 4 and 6 Months of Age, Compared to That of the Routine Pediatric Vaccines Co-administered With Placebo Secondary · Day 1 to 12 months post-dose 1

Percentage of participants with medically attended adverse events (AEs) at any point during the trial.

GroupValue95% CI
Placebo63.4
HIL-21465.5

Adverse events — posted to ClinicalTrials.gov

Time frame: Serious adverse events were assessed from Day 1 to 12 months post-dose 1; unsolicited adverse events were assessed for up to 28 days post-dose 1 and 28 days post-dose 2.. Reporting threshold: 3%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Placebo
Serious: 7/164 (4%)
Deaths: 0/164
HIL-214
Serious: 3/165 (2%)
Deaths: 0/165

Serious adverse events (5 terms)

ReactionSystemPlaceboHIL-214
BronchiolitisInfections and infestations
Pneumonia respiratory syncytial virusInfections and infestations
PneumoniaInfections and infestations
Urinary tract infectionInfections and infestations
Acute respiratory failureRespiratory, thoracic and mediastinal disorders
Other adverse events (4 terms — click to expand)

ReactionSystemPlaceboHIL-214
NasopharyngitisInfections and infestations
GastroenteritisInfections and infestations
BronchiolitisInfections and infestations
PyrexiaGeneral disorders

Most-reported serious reactions: Bronchiolitis, Pneumonia respiratory syncytial virus, Pneumonia, Urinary tract infection, Acute respiratory failure.

Data from ClinicalTrials.gov NCT05836012 adverse events section.

Sponsor's own description

This is a phase 2, multi-country, randomized, double-blind, placebo-controlled trial to evaluate the immune response to routine pediatric vaccinations when co-administered with HIL-214 or placebo in healthy infants. This trial will also evaluate the safety profile of a 2-dose regimen of HIL-214 co-administered with routine pediatric vaccines.

Publications & conference data

2 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Engineering <i>Escherichia coli</i>-Derived Nanoparticles for Vaccine Development.
    Tang S, Zhao C, Zhu X. · · 2024 · cited 4× · PMID 39591189 · DOI 10.3390/vaccines12111287
  2. The Impact of Norovirus on Children and Adolescents: Implications for Ongoing Vaccine Development.
    Tan M. · · 2024 · PMID 40823381 · DOI 10.1007/s40124-025-00355-9

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Other trials of HIL-214

Trials testing the same drug.

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Trials by the same sponsor.

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Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing