NCT05826496: INHALE

Exercise-induced Effects on Immune Parameters in Healthy Participants

Completed NA Last updated 12 February 2025

What this trial tests

NA trial testing Exercise in Healthy in 59 participants. Completed in 10 February 2025.

Timeline

15 March 2023

Primary endpoint
31 December 2024

10 February 2025

Quick facts

Lead sponsor	Per thor Straten
Phase	NA
Status	Completed
Study type	INTERVENTIONAL
Allocation	na
Design	single group
Masking	none
Primary purpose	basic science
Enrollment	59
Start date	15 March 2023
Primary completion	31 December 2024
Estimated completion	10 February 2025
Sites	1 location across Denmark

Drugs / interventions tested

• Exercise

Conditions studied

• Healthy — all drugs for Healthy →

Sponsor

Per thor Straten

Who can join

Adults 18 to 75, any sex, with Healthy. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Exercise has been shown to influence the immune system and, for example, improve anti-viral immune response. However, knowledge of how exercise impacts the immune system is still lacking. Therefore, the goal of this clinical study is to perform a comprehensive multi-parameter analysis of immunological parameters in healthy participants before and after one bout of high-intensity aerobic exercise. The primary endpoint of this study is to determine the exercise-induced changes of anti-viral T cell immunity in peripheral blood against common and recurrent viruses.

Up to 70 healthy participants in the age between 18 and 75 will be recruited. The first visit will be for prescreening the health status, answering questionnaires and providing a capillary blood sample for HLA screening. HLA-A2 positive participants will continue on the trial with a VO2 max test for Visit 2 and the supervised aerobic medium- to high-intensity (90% VO2 max) exercise session for Visit 3. Peripheral blood samples will be taken pre-exercise, within 2 minutes post-exercise and 60 minutes post-exercise.

These findings may pave the way to define serum markers or cellular immunological traits that provide new insight into how exercise promotes powerful and sustained cellular immune responses.

Publications & conference data

3 peer-reviewed publications reference this trial (live from Europe PMC):

1. Exercise Duration Modulates Cortisol Release and Chronic Cortisol Exposure Jeopardises T Cell Effector Functions.
   Luu TV, Fleischer Hach L, Seremet T, Leuchte K, et al · · 2026 · cited 2× · PMID 40796353 · DOI 10.1111/imm.70028
2. Chemokine Receptor Profile of Circulating Leukocyte Subsets in Response to Acute High-Intensity Interval Training.
   Leuchte K, Fresnillo Saló S, Rahbech A, Byrdal M, et al · · 2026 · PMID 41750333 · DOI 10.3390/biom16020263
3. Moving for optimal immunity: the effect of acute high-intensity interval training on phenotype, virus specificity and chemokine receptor expression in human CD8+ T cells.
   Leuchte K, Luu TV, Fresnillo Saló S, Madsen K, et al · · 2025 · PMID 41675500 · DOI 10.3389/fimmu.2025.1739657