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Integrated Proteomic HPLC-ESI-MS Analysis of Salivary Peptides of Preterm Newborns: a Pilot Study
The analysis of saliva of preterm newborns could be a powerful tool to investigate human fetal development in an ethically acceptable fashion, indeed the collection of salivary samples is a fast and non-invasive procedure. The purpose of the study is to characterize peptide and proteins present in human preterm saliva and to investigate the relative amount of several proteoforms of the proteins and peptides detectable in preterm saliva in order to have information on the activity of various enzymes acting during late fetal development. Preterm infants with gestational age between 175-216 days (25-30 weeks), admitted to the Neonatal Intensive Care Unit (NICU) will be enrolled for this study. A saliva sample will be collected every seven days from the birthday and up to 40 weeks (286 days) of postmenstrual age (PMA) or up to discharge if it occurs earlier. A targeted ESI mass spectrometry investigation, based on a top-down analysis of the intact salivary proteome will be performed.
Details
| Lead sponsor | Fondazione Policlinico Universitario Agostino Gemelli IRCCS |
|---|---|
| Status | COMPLETED |
| Enrolment | 30 |
| Start date | Thu Feb 17 2022 00:00:00 GMT+0000 (Coordinated Universal Time) |
| Completion | Thu Mar 30 2023 00:00:00 GMT+0000 (Coordinated Universal Time) |
Conditions
- Bronchopulmonary Dysplasia; Retinopathy of Prematurity
Interventions
- Integrated proteomic HPLC-ESI-MS analysis of salivary peptides
Countries
Italy