NCT05711407

Peri-implantitis and MMP-8

Completed Last updated 16 April 2025

What this trial tests

trial testing Gene Polymorphisms of MMP-8 in Peri-Implantitis in 100 participants. Completed in 1 January 2025.

Timeline

3 March 2023

Primary endpoint
1 January 2024

1 January 2025

Quick facts

Lead sponsor	Aristotle University Of Thessaloniki
Status	Completed
Study type	OBSERVATIONAL
Enrollment	100
Start date	3 March 2023
Primary completion	1 January 2024
Estimated completion	1 January 2025
Sites	1 location across Greece

Drugs / interventions tested

• Gene Polymorphisms of MMP-8

Conditions studied

• Peri-Implantitis — all drugs for Peri-Implantitis →

Sponsor

Aristotle University Of Thessaloniki

Who can join

Eligibility, male only, with Peri-Implantitis. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Peri-implantitis is defined as the pathological condition around dental implants characterized by inflammation in the peri-implant mucosa and progressive bone loss, eventually leading to implant loss. Peri-implantitis is thought to be a disease analogous to periodontitis with a prevalence reaching 22%. Though peri-implantitis is readily recognized as a part of modern dentistry, the exact etiology or an effective treatment regimen hasn't been established yet. Thus, contemporary research is orientating toward acknowledging the aetiologic and risk factors of the disease and of course establishing prognostic markers for disease prevention. Microbiota residing in the subgingival plaque are considered the main etiologic factor of the disease, however, current literature has not concluded on the exact microbial composition of peri-implant lesions. In addition, genetic predisposition has been recognized as a risk factor for disease initiation and progression and several observational studies have addressed the potential association between various gene polymorphisms and the occurrence of peri-implantitis. Lastly, to establish effective preventive measures, several biomarkers have been evaluated as potential diagnostic and prognostic markers of disease progression.

Objectives:

1. To identify the relationship of peri-implantitis with Cycloxygenase-2 (COX-2) and MMP-8 gene polymorphisms. Cyclooxygenase catalyzes the production of prostaglandins (PGs) which are an important inflammatory mediator participating in the pathogenesis of peri-implantitis. In addition, PGE2 expression in the peri-implant crevicular fluid will be assessed.
2. To characterize the microbiota associated with peri-implantitis lesions, using novel identification techniques enabling the identification of specific opportunistic bacteria associated with the disease.
3. To test the diagnostic accuracy of a modern chairside test, using metalloproteinase-8 (MMP-8), an enzyme implicated in the pathogenesis of the disease, as a biomarker of disease progression.

Publications & conference data

4 peer-reviewed publications reference this trial (live from Europe PMC):

1. Diagnostic Accuracy of Active MMP-8 Point-of-Care Test in Peri-Implantitis.
   Fragkioudakis I, Batas L, Vouros I, Sakellari D. · · 2025 · cited 2× · PMID 39657936 · DOI 10.1055/s-0044-1793843
2. Association of MMP-8 -799C/T Polymorphism with Peri-Implantitis: A Cross-Sectional Study.
   Fragkioudakis I, Kottaridi C, Doufexi AE, Papadimitriou K, et al · · 2025 · cited 1× · PMID 40423054 · DOI 10.3390/jpm15050182
3. MMP-8 in Peri-Implantitis: A Cross-Sectional Study on Genetic Polymorphisms and Enzymatic Activation.
   Fragkioudakis I, Kottaridi C, Paraskeva M, Sakellari D. · · 2026 · PMID 41782448 · DOI 10.1111/jre.70026
4. Quantitative real-time PCR detection of <i>Porphyromonas gingivalis</i> and <i>Filifactor alocis</i> in peri-implantitis.
   Fragkioudakis I, Konstantopoulos G, Kottaridi C, Batas L, et al · · 2025 · PMID 41222987 · DOI 10.1099/jmm.0.002091