NCT05396963

Egg White Supplementation in High-Intensity Functional Training

Completed NA Last updated 5 October 2023

What this trial tests

NA trial testing Egg white in Exercise Training in 40 participants. Completed in 30 September 2023.

Timeline

1 November 2021

Primary endpoint
31 March 2023

30 September 2023

Quick facts

Lead sponsor	Aristotle University Of Thessaloniki
Phase	NA
Status	Completed
Study type	INTERVENTIONAL
Allocation	randomized
Design	crossover
Masking	triple
Primary purpose	other
Enrollment	40
Start date	1 November 2021
Primary completion	31 March 2023
Estimated completion	30 September 2023
Sites	1 location across Greece

Drugs / interventions tested

• Egg white
• Whey protein
• Maltodextrin (MALTODEXTRIN) — full drug profile →

Conditions studied

• Exercise Training — all drugs for Exercise Training →
• Dietary Supplements — all drugs for Dietary Supplements →

Sponsor

Aristotle University Of Thessaloniki

Who can join

Adults 18 to 65, any sex, with Exercise Training or Dietary Supplements. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

High-intensity functional training (HIFT), a new version of high-intensity interval training, has gained interest in recent years. HIFT is based on the CrossFit training template and includes multijoint movement patterns via both endurance and strengthening exercises. Research has shown positive effects of HIFT on body composition, cardiorespiratory fitness, and muscle performance of young individuals. The effectiveness of HIFT in higher ages and its protein requirements are less well documented. Protein requirements have been widely investigated in resistance training, where it has been found that protein supplementation may have additive beneficial effects on muscle strength and lean body mass. However, there is a research gap regarding the adaptations to HIFT when combined with protein supplementation.

Thus, the aim of this research is to compare the effects of egg white supplementation, whey protein supplementation (as a positive comparator), and maltodextrin (a carbohydrate as placebo) on body composition, physical performance, and plasma amino acid profile in young and middle-aged trained individuals of both sexes who perform HIFT. Participants will take all three supplements for 6 weeks each, with 2 weeks of washout (no supplementation) in between, in random and counterbalanced order. Researchers will not know the supplementation status of the participants. Participants will receive 0.6 g of protein or placebo per kg body weight daily on top of isoenergetic dietary plans to avoid differences in energy intake that might compromise the validity of the study. The dietary plans will be individualized and will provided 1.0 g protein/kg body weight/day. The HIFT protocol will include multimodal patterns of movement, combining endurance and strengthening exercises with the use of equipment such as Total Resistance eXercise (TRX), Bosu, kettlebells, and barbells.

Participants will undergo measurements of muscle strength, muscle endurance, aerobic capacity, and body composition at the beginning and end of the study, as well as during the two washout periods. Also, plasma amino acids, hematology, biochemistry, and hormones will be measured. Comparison of all these outcome measures between supplements will reveal whether protein supplementation is useful in HIFT.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

1. Effects of Protein Supplementation During High-Intensity Functional Training on Physical Performance in Recreationally Trained Males and Females: A Randomized Controlled Trial.
   Karpouzi C, Kosmidis I, Petridou A, Voulgaridou G, et al · · 2025 · cited 1× · PMID 40362749 · DOI 10.3390/nu17091441