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NCT05242965

A Multiple Antigen Vaccine (STEMVAC) for the Treatment of Patients With Stage IV Non-Small Cell Lung Cancer

Active, enrolled Phase 2 Results posted Last updated 3 April 2026
What this trial tests

Phase 2 trial testing CD105/Yb-1/SOX2/CDH3/MDM2-polyepitope Plasmid DNA Vaccine in Lung Non-Small Cell Carcinoma in 5 participants. Participants enrolled and being followed up; not accepting new ones.

Timeline
24 March 2023
Primary endpoint
13 August 2024
31 December 2026

Quick facts

Lead sponsorUniversity of Washington
PhasePhase 2
StatusActive, enrolled
Study typeINTERVENTIONAL
Allocationrandomized
Designsequential
Maskingdouble
Primary purposetreatment
Enrollment5
Start date24 March 2023
Primary completion13 August 2024
Estimated completion31 December 2026
Sites2 locations across United States

Drugs / interventions tested

Conditions studied

Sponsor

University of Washington

Who can join

18 and older, any sex, with Lung Non-Small Cell Carcinoma or Stage IV Lung Cancer AJCC v8. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

CD8 is Reported as a Fraction of CD3+ Tumor Infiltrating Lymphocytes (TIL) in 4 High-power Field (HPF) Primary · Baseline and after the third vaccine (at approximately 12 weeks)

Immunohistochemical (IHC) staining for CD8+ CD3+ will be performed on the biopsies collected pre-treatment and post 3rd vaccine administration.

Baseline % of CD8+ of CD3+ TIL
GroupValue95% CI
Arm I (STEMVAC, Sargramostim)3232 – 32
Arm II (Sargramostim)3214 – 51
Post Vaccine % of CD8+ of CD3+ TIL
GroupValue95% CI
Arm I (STEMVAC, Sargramostim)4949 – 49
Arm II (Sargramostim)4126 – 56
Number of Participants With Recorded Adverse Event(s) Primary · Up to 20 weeks

Will be evaluated using the modified National Cancer Institute (NCI) toxicity criteria. Toxicity evaluation will be based on Common Terminology Criteria for Adverse Events (CTCAE) v5.0

GroupValue95% CI
Arm I (STEMVAC, Sargramostim)2
Arm II (Sargramostim)3

Adverse events — posted to ClinicalTrials.gov

Time frame: Systemic adverse events (AE) will be recorded from the time of confirmed eligibility through End of Treatment (up to 20 weeks). Patients are monitored for the development of vaccine toxicities with clinical lab results and physical exams. Clinically significant AEs are graded on a scale of 1-5. We will record AEs reported to us by the patient, caregivers, and clinical support staff. All-cause mortality will be assessed up to 5 years. Serious and other AEs were assessed up to 20 weeks.. Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Arm I (STEMVAC, Sargramostim)
Serious: 0/2 (0%)
Deaths: 0/2
Arm II (Sargramostim)
Serious: 0/3 (0%)
Deaths: 2/3
Other adverse events (39 terms — click to expand)

ReactionSystemArm I (STEMVAC, Sargramost…Arm II (Sargramostim)
DyspneaRespiratory, thoracic and mediastinal disorders
Injection Site ReactionGeneral disorders
AnemiaBlood and lymphatic system disorders
Neutrophil count decreasedInvestigations
Creatinine increasedInvestigations
Nasal CongestionRespiratory, thoracic and mediastinal disorders
Blurred VisionEye disorders
Dry EyeEye disorders
Watering EyesEye disorders
Upper Respiratory InfectionInfections and infestations
HeadacheNervous system disorders
Accidental Opioid OverdoseNervous system disorders
DiarrheaGastrointestinal disorders
NauseaGastrointestinal disorders
Abdominal PainGastrointestinal disorders
Flu Like SymptomsGeneral disorders
PainGeneral disorders
Minor Contusion, Left ForeheadInjury, poisoning and procedural complications
HypokalemiaMetabolism and nutrition disorders
InsomniaPsychiatric disorders
TinnitusEar and labyrinth disorders
Hemoptysis After Lung BiopsyRespiratory, thoracic and mediastinal disorders
T-10 Spine Tingling with NumbnessMusculoskeletal and connective tissue disorders
Muscle Cramping, Bilateral LegsMusculoskeletal and connective tissue disorders
Tinea PedisRespiratory, thoracic and mediastinal disorders
Skin InfectionInfections and infestations
PalpitationsCardiac disorders
DizzinessNervous system disorders
Peripheral Sensory NeuropathyNervous system disorders
SomnolenceNervous system disorders
BruisingInjury, poisoning and procedural complications
HypotensionVascular disorders
AnorexiaMetabolism and nutrition disorders
HypoalbuminemiaMetabolism and nutrition disorders
DehydrationMetabolism and nutrition disorders
HyperglycemiaMetabolism and nutrition disorders
Urinary IncontinenceRenal and urinary disorders
Decrease Sensation with UrinationRenal and urinary disorders
Urinary UrgencyRenal and urinary disorders

Data from ClinicalTrials.gov NCT05242965 adverse events section.

Sponsor's own description

This phase II trial tests whether CD105/Yb-1/SOX2/CDH3/MDM2-polyepitope plasmid DNA vaccine (STEMVAC) works to shrink tumors in patients with stage IV non-small cell lung cancer. STEMVAC targets specific immunogenic proteins that help lung cancer cells to grow. STEMVAC is made up of deoxyribonucleic acid (DNA), which is a natural substance in every living organism. DNA acts like a blueprint that tells all the cells in your body how to function. The DNA used in this study contains instructions for your body to produce parts of the 5 proteins the investigators identified (CDH3, CD105, YB-1, MDM2 and SOX2). STEMVAC is given with granulocyte-macrophage colony stimulating factor (GM-CSF) which is being used as an adjuvant to help create a stronger immune response. Giving STEMVAC with GM-CSF to patients while on maintenance therapy for non-small cell lung cancer (NSCLC) may help activate certain immune cells to recognize and kill lung cancer cells.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Mechanisms of resistance to targeted therapy and immunotherapy in non-small cell lung cancer: promising strategies to overcoming challenges.
    Xiang Y, Liu X, Wang Y, Zheng D, et al · · 2024 · cited 85× · PMID 38655260 · DOI 10.3389/fimmu.2024.1366260
  2. The next-generation DNA vaccine platforms and delivery systems: advances, challenges and prospects.
    Lu B, Lim JM, Yu B, Song S, et al · · 2024 · cited 84× · PMID 38361919 · DOI 10.3389/fimmu.2024.1332939
  3. Immunotherapy resistance in non-small-cell lung cancer: From mechanism to clinical strategies.
    Zhou S, Yang H. · · 2023 · cited 64× · PMID 37090727 · DOI 10.3389/fimmu.2023.1129465
  4. The future of cancer immunotherapy: DNA vaccines leading the way.
    Pandya A, Shah Y, Kothari N, Postwala H, et al · · 2023 · cited 53× · PMID 37294501 · DOI 10.1007/s12032-023-02060-3
  5. Neoantigen Vaccines; Clinical Trials, Classes, Indications, Adjuvants and Combinatorial Treatments.
    Niemi JVL, Sokolov AV, Schiöth HB. · · 2022 · cited 42× · PMID 36291947 · DOI 10.3390/cancers14205163
  6. Y-Box Binding Protein 1: Unraveling the Multifaceted Role in Cancer Development and Therapeutic Potential.
    Dinh NTM, Nguyen TM, Park MK, Lee CH. · · 2024 · cited 37× · PMID 38255791 · DOI 10.3390/ijms25020717
  7. Differences between lung adenocarcinoma and lung squamous cell carcinoma: Driver genes, therapeutic targets, and clinical efficacy.
    Shen Y, Chen JQ, Li XP. · · 2025 · cited 26× · PMID 40083325 · DOI 10.1016/j.gendis.2024.101374
  8. Advances in nucleic acid-based cancer vaccines.
    Liao HC, Liu SJ. · · 2025 · cited 25× · PMID 39833784 · DOI 10.1186/s12929-024-01102-w

Verify or expand the search:

Other trials of CD105/Yb-1/SOX2/CDH3/MDM2-polyepitope Plasmid DNA Vaccine

Trials testing the same drug.

Other recruiting trials for Lung Non-Small Cell Carcinoma

Currently open trials in the same condition.

Other University of Washington trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT05242965.

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