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NCT05057923

The Development of a COVID19 Oral Vaccine Consisting of Bacillus Subtilis Spores

Completed NA Results posted Last updated 31 May 2023
What this trial tests

NA trial testing Bacillus subtilis in COVID-19 Pneumonia in 9 participants. Completed in 1 November 2021.

Timeline
10 July 2021
Primary endpoint
20 October 2021
1 November 2021

Quick facts

Lead sponsorDreamTec Research Limited
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationnon randomized
Designparallel
Maskingnone
Primary purposebasic science
Enrollment9
Start date10 July 2021
Primary completion20 October 2021
Estimated completion1 November 2021
Sites1 location across Hong Kong

Drugs / interventions tested

Conditions studied

Sponsor

DreamTec Research Limited — full company profile →

Who can join

25 and older, any sex, with COVID-19 Pneumonia. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Serum Neutralizing Receptor Binding Domain IgG Antibody Concentration Primary · Day 0, 27, 42 post oral administration

Concentration of neutralizing IgG antibody against receptor binding domain of spike protein in SARS-COV2

Day 0
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated Participants0.2± 0.1
Day 27
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated Participants9.3± 5.4
Day 42
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated Participants35.8± 8.8
Lentirival Pseudovirus Neutralization Assay (Wild Type of SARS-CoV2) Secondary · Day 0, 27, 42 post oral administration

The ability of neutralization against SARS-CoV-2 was tested by an in vitro pseudo-virus neutralization assay. The lentivirus carrying a GFP gene was pseudotyped with the spike protein from a wild type of SARS-CoV-2. The pseudoviruses were then pre-incubated with serially diluted serum samples from orally vaccinated volunteers before being added to A549 lung carcinoma cells expressing human ACE2 and human TMPRSS2. The percentage of infection rate was measured with a fluorescent plate reader by counting GFP-positive cells. The results were fitted with a non-linear regression model. The dilution

EC50 of Day 27 Serum diltuion in Pseudovirus inhibition assay
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated ParticipantsNA± NA
EC50 of Day 0 Serum diltuion in Pseudovirus inhibition assay
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated ParticipantsNA± NA
EC50 of Day 42 Serum diltuion in Pseudovirus inhibition assay
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated Participants76.33± 38.7
Lentirival Pseudovirus Neutralization Assay (D614G SARS-COV2 Variant) Secondary · Day 0, 27, 42 post oral administration

The ability of neutralization against SARS-CoV-2 was tested by an in vitro pseudo-virus neutralization assay. The lentivirus carrying a GFP gene was pseudotyped with the spike protein from a D614G variant of SARS-CoV-2. The pseudoviruses were then pre-incubated with serially diluted serum samples from orally vaccinated volunteers before being added to A549 lung carcinoma cells expressing human ACE2 and human TMPRSS2. The percentage of infection rate was measured with a fluorescent plate reader by counting GFP-positive cells. The results were fitted with a non-linear regression model. The dilut

EC50 of Day 27 Serum diltuion in Pseudovirus inhibition assay
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated ParticipantsNA± NA
EC50 of Day 0 Serum diltuion in Pseudovirus inhibition assay
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated ParticipantsNA± NA
EC50 of Day 42 Serum diltuion in Pseudovirus inhibition assay
GroupValue95% CI
Generation of Neutralizing Antibody for Unvaccinated Participants82.4± 29.2

Sponsor's own description

This is a study trial to assess the effectiveness of the immune response stimulated by the genetically engineered Bacillus subtilis which express and display Spike protein of the SARS-COV2 on the spore coat.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

  1. Expression of SARS-CoV-2 Spike Protein Receptor Binding Domain on Recombinant <i>B. subtilis</i> on Spore Surface: A Potential COVID-19 Oral Vaccine Candidate.
    Sung JC, Liu Y, Wu KC, Choi MC, et al · · 2021 · cited 7× · PMID 35062663 · DOI 10.3390/vaccines10010002

Verify or expand the search:

Other trials of Bacillus subtilis

Trials testing the same drug.

Other recruiting trials for COVID-19 Pneumonia

Currently open trials in the same condition.

Other DreamTec Research Limited trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT05057923.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing