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NCT05027958

Characterization of Tissue-Specific Immune Responses to Bronchoscopic Instillation of Mycobacterial Antigens Into the Human Lung

Completed EARLY_PHASE1 Results posted Last updated 6 November 2025
What this trial tests

EARLY_PHASE1 trial testing Tuberculin Purified Protein Derivative in Healthy in 17 participants. Completed in 8 December 2024.

Timeline
3 May 2022
Primary endpoint
7 November 2024
8 December 2024

Quick facts

Lead sponsorNational Heart, Lung, and Blood Institute (NHLBI)
PhaseEARLY_PHASE1
StatusCompleted
Study typeINTERVENTIONAL
Allocationnon randomized
Designparallel
Maskingsingle
Primary purposebasic science
Enrollment17
Start date3 May 2022
Primary completion7 November 2024
Estimated completion8 December 2024
Sites1 location across United States

Drugs / interventions tested

Conditions studied

Sponsor

National Heart, Lung, and Blood Institute (NHLBI)

Who can join

Adults 18 to 64, any sex, with Healthy or Latent Tuberculosis Infection (LTBI). Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Median Difference in CD4 Cell Response to Intrabronchial Instillation of Tuberculin Purified Protein Derivative (PPD) Primary · Day 5 and Day 21

Median difference in CD4 cell response to intrabronchial instillation of Tuberculin Purified Protein Derivative (PPD) using flow cytometry. The frequency of (Mycobacterium tuberculosis) Mtb-specific T cells was measured by intracellular cytokine staining for Interferon Gamma (IFNγ) and Tumor Necrosis Factor (TNF) after in vitro restimulation of Peripheral Blood Mononuclear Cells (PBMC) and Bronchoalveolar Lavage (BAL) cells with PPD or Mtb-derived peptide megapools.

Day 5
GroupValue95% CI
Arm 1: Healthy Volunteer Participants With Confirmed Latent Tuberculosis Infection (LTBI)5.263.99 – 11.5
Arm 2: Healthy Volunteer Participants With Confirmed Non-Latent Tuberculosis Infection (LTBI)0.7250.462 – 1.74
Day 21
GroupValue95% CI
Arm 1: Healthy Volunteer Participants With Confirmed Latent Tuberculosis Infection (LTBI)29.919 – 37.3
Arm 2: Healthy Volunteer Participants With Confirmed Non-Latent Tuberculosis Infection (LTBI)2.921.98 – 4.16

Adverse events — posted to ClinicalTrials.gov

Time frame: 30 Days. Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Arm 1: Healthy Volunteer Participants With Confirmed Latent Tuberculosis Infection (LTBI)
Serious: 0/7 (0%)
Deaths: 0/7
Arm 2: Healthy Volunteer Participants With Confirmed Non-Latent Tuberculosis Infection (LTBI)
Serious: 0/10 (0%)
Deaths: 0/10
Other adverse events (46 terms — click to expand)

ReactionSystemArm 1: Healthy Volunteer P…Arm 2: Healthy Volunteer P…
CoughRespiratory, thoracic and mediastinal disorders
Sore throatRespiratory, thoracic and mediastinal disorders
BruisingInjury, poisoning and procedural complications
HeadacheNervous system disorders
HoarsenessRespiratory, thoracic and mediastinal disorders
HypertensionVascular disorders
Sinus bradycardiaCardiac disorders
NauseaGastrointestinal disorders
Nasal congestionRespiratory, thoracic and mediastinal disorders
WheezingRespiratory, thoracic and mediastinal disorders
HypotensionVascular disorders
AnemiaBlood and lymphatic system disorders
White blood cell decreasedBlood and lymphatic system disorders
Sinus tachycardiaCardiac disorders
Red eyesEye disorders
BruisingGeneral disorders
ChillsGeneral disorders
Edema faceGeneral disorders
FatigueGeneral disorders
FeverGeneral disorders
Injection site reactionGeneral disorders
Non-cardiac chest painGeneral disorders
PainGeneral disorders
Injection site reactionInjury, poisoning and procedural complications
Skin lacerationInjury, poisoning and procedural complications
Blood bicarbonate decreasedInvestigations
Basophil count increasedInvestigations
C-Reactive Protein increasedInvestigations
Lymphocyte count decreasedInvestigations
HyperglycemiaMetabolism and nutrition disorders
Back painMusculoskeletal and connective tissue disorders
DizzinessNervous system disorders
HypersomniaNervous system disorders
Gag reflexNervous system disorders
Urinary retentionRenal and urinary disorders
Adnexal cystic massReproductive system and breast disorders
DyspneaRespiratory, thoracic and mediastinal disorders
Productive coughRespiratory, thoracic and mediastinal disorders
BradypneaRespiratory, thoracic and mediastinal disorders
Lung consolidationsRespiratory, thoracic and mediastinal disorders

Data from ClinicalTrials.gov NCT05027958 adverse events section.

Sponsor's own description

Background: Tuberculosis (TB) is a lung disease. It is caused by inhaling a type of airborne bacterium. Tuberculin Purified Protein Derivative (PPD) is used to test for TB exposure. It is usually injected under a person s skin. In this study, it will be applied in the lung. Objective: To learn how the cells within the lung react (immune response) when exposed to PPD. Eligibility: Adults ages 18-64 who (1) have been exposed to TB but do not have active disease or symptoms or (2) have never been exposed to TB. Design: Participants will be screened with a medical history, physical exam, and blood tests. They will have a TB skin test. They will also have an electrocardiogram to examine heart rhythm. For this, sticky patches will be placed on their chest. Some screening tests will be repeated at study visits. Participants will have 3 FDG PET-CT scans. They will lie in a machine that creates pictures of the inside of their body. They will get a radioactive substance injected into their arm called 18FDG. It helps make the pictures. Participants will have 3 bronchoscopies. Their mouth and nasal airways will be numbed. They will get drugs to relax. A tube will be inserted through their nose or mouth into a lung. Fluid will be delivered into the lung and suctioned back out to collect cells. They will get PPD during the first bronchoscopy. Participation will last for about 30 days. Participants will visit the clinic up to 8 times. They will go home after each procedure. No hospital stays are needed....

Publications & conference data

No peer-reviewed publications indexed yet for this trial. Completed trials usually publish results within 12-18 months.

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Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT05027958.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing