NCT04985006

Effect of Exercise Intensity on Epigenetic Response in Healthy Young Adult

Status unknown NA Last updated 22 September 2021

What this trial tests

NA trial testing running at moderate intensity in Healthy in 15 participants. Status unknown.

Timeline

1 October 2020

Primary endpoint
1 December 2021

1 February 2022

Quick facts

Lead sponsor	Rahmaningsih Mara Sabirin
Phase	NA
Status	Status unknown
Study type	INTERVENTIONAL
Allocation	randomized
Design	crossover
Masking	single
Primary purpose	basic science
Enrollment	15
Start date	1 October 2020
Primary completion	1 December 2021
Estimated completion	1 February 2022
Sites	1 location across Indonesia

Drugs / interventions tested

• running at moderate intensity
• running at high intensity

Conditions studied

• Healthy — all drugs for Healthy →

Sponsor

Rahmaningsih Mara Sabirin

Who can join

Adults 18 to 25, male only, with Healthy. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Aerobic exercise has been shown to trigger a variety of body responses which then trigger physiological adaptations. One of the physiological adaptations that occur is an increase in mitochondrial biogenesis. Mitochondria are organelles in cells that play a role in providing energy. An increase in the number of mitochondria will increase the supply of energy for muscle cell contraction, so that muscles do not get tired easily.

Mitochondrial biogenesis is known to be regulated by Peroxisome proliferator-activated receptor gamma co-activator-1 alpha (PGC-1α), by inducing transcription of genes encoded by the nucleus and mitochondria. Peroxisome proliferator-activated receptor gamma co-activator-1 alpha is a protein which encoded by the PPARGC1A gene. PPARGC1A gene expression itself can be influenced by microRNA, a short non-coding RNA that can regulate gene expression by suppressing or degrading the target gene. In vitro studies show that PPARGC1A gene is a direct gene target of microRNA (miR)-23a.

In human studies, aerobic exercise has been shown to reduce miR-23a levels in human muscles immediately after exercise. Another study found an increase in muscle PGC-1 alpha levels after exercise. In addition to being detectable in muscle, both miR-23a and PGC-1 alpha can be measured in blood plasma. However, whether exercise can reduce miR-23a levels while increasing PGC-1 alpha levels in circulation, still needs further research. Therefore, this study aims to examine whether aerobic exercise will reduce miR-23a levels and PGC-1 alpha levels in plasma. In addition, this study will also compare whether exercise intensity affects the expression of miR-23a and PGC-1 alpha.

In this study, measurements will be made on blood plasma to reduce the level of invasiveness. Subjects will be asked to come to the laboratory three times. The first meeting consisted of an examination, while in the second and third meetings the subjects would be asked to run at a moderate or high intensity. Blood sampling will be done before and after exercise.

Publications & conference data

No peer-reviewed publications indexed yet for this trial.