NCT04936737

The Influence of Exercise on Tissue Beta-alanine Uptake and Carnosine Synthesis Rates

Status unknown NA Last updated 23 June 2021

What this trial tests

NA trial testing Beta-alanine supplementation in Exercise Training in 14 participants. Status unknown.

Timeline

6 May 2021

Primary endpoint
23 December 2022

31 December 2023

Quick facts

Lead sponsor	University of Sao Paulo
Phase	NA
Status	Status unknown
Study type	INTERVENTIONAL
Allocation	randomized
Design	crossover
Masking	none
Primary purpose	basic science
Enrollment	14
Start date	6 May 2021
Primary completion	23 December 2022
Estimated completion	31 December 2023
Sites	1 location across Brazil

Drugs / interventions tested

• Beta-alanine supplementation
• High intensity interval exercise

Conditions studied

• Exercise Training — all drugs for Exercise Training →
• Carnosine — all drugs for Carnosine →
• Dietary Supplements — all drugs for Dietary Supplements →

Sponsor

University of Sao Paulo

Who can join

Adults 18 to 40, male only, with Exercise Training or Carnosine. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

B-alanine supplementation is highly efficient in increasing intramuscular carnosine content, leading to improved physical performance, especially in high-intensity exercises (HIIE). It seems that exercise per se can modulate carnosine content; however, it remains uncertain whether physical training or training status can influence B-alanine supplementation responses. Thus, this work aims to assess whether HIIE can increase B-alanine uptake by peripheral tissues and, more specifically, skeletal muscle and increase intramuscular carnosine synthesis. The volunteers will be evaluated in two conditions: intake of B-alanine + exercise (B-EX) and B-alanine intake only (B-Ala). This process will be divided into two blocks of six days (Thursday to Thursday - without the weekend) with a 4-6 weeks washout. In the B-EX block, a 20-minute HIIE session will be held. In the B-Ala block, the same procedures will be adopted for the B-EX block, with the replacement of the HIIE for 20 minutes of rest sitting on the cycle ergometer. We will evaluate the determination of muscle B-alanine, plasma, and urine, the gene expression of carnosine-related enzymes and transporters, the enzymes Carnosine Synthase 1 (CARNS1), carnosine dipeptidase 2 (CN2), taurine transporter (TauT), PAT1, and phosphorylated Na + / K + / ATPase. The hypotheses are: 1) acute physical exercise increases the uptake of B-alanine by the skeletal muscle; 2) this effect is mediated by the increased activity of the Na + / K + / ATPase pump; 3) this effect, when repeated over five training sessions, results in observable increases in β-alanine → carnosine conversion in skeletal muscle.

Publications & conference data

No peer-reviewed publications indexed yet for this trial.