NCT04427371

The Effect and Mechanism of lncRNA NBR2 Regulating Endothelial Pyroptosis by Targeting GSDMD in Sepsis

Status unknown Last updated 11 June 2020

What this trial tests

trial testing the levels of pyroptosis in Sepsis in 100 participants. Status unknown.

Timeline

22 May 2020

Primary endpoint
31 December 2022

31 December 2023

Quick facts

Lead sponsor	Jianfeng Xie
Status	Status unknown
Study type	OBSERVATIONAL
Enrollment	100
Start date	22 May 2020
Primary completion	31 December 2022
Estimated completion	31 December 2023
Sites	1 location across China

Drugs / interventions tested

• the levels of pyroptosis

Conditions studied

• Sepsis — all drugs for Sepsis →

Sponsor

Jianfeng Xie

Who can join

Adults 18 to 80, any sex, with Sepsis. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Sepsis is a life-threatening disease characterized by multi-organ failure due to dysregulated host response to infection, with high global mortality of 30-50%. One of the most important pathophysiologic hallmarks in sepsis is vascular endothelial injury that contributes to the severity and outcome of the syndrome. Effective treatments for endothelial cell injury in sepsis have been lacking to improve prognosis. Endothelial pyroptosis is a vital mechanism of vascular endothelial injury in sepsis; mitigation or abolishment of endothelial cell pyroptosis alleviate vascular endothelial damage and improve the prognosis of sepsis mice. Gasdermin D (GSDMD) mediated endothelial pyroptosis plays a critical role in modulating vascular endothelial injury in sepsis.

Long non-coding RNA (lncRNA), as a class of non-coding protein RNA longer than 200 kD, contributes to a variety of cell biological processes. The dysregulation of lncRNA results in the occurrence and development of tumors, diabetes, sepsis and other diseases. Therefore, we detected lncRNA and mRNA expression profile in 26 blood samples of septic patients using Arraystar LncRNA Microarray. We found lncRNA NBR2 regulates septic endothelial pyroptosis.

To assess any correlation of pyroptosis levels with relevant endothelial cell injury parameters and determine the prognostic value in septic patients. we measured the levels of pyroptosis in patients admitted to the Department of Critical Care Medicine for sepsis and investigated the correlation with related markers of endothelium injury. Furthermore, we determined the prognostic value of pyroptosis levels for the mortality of patients with sepsis.

Publications & conference data

5 peer-reviewed publications reference this trial (live from Europe PMC):

1. Mitochondria-associated programmed cell death as a therapeutic target for age-related disease.
   Nguyen TT, Wei S, Nguyen TH, Jo Y, et al · · 2023 · cited 221× · PMID 37612409 · DOI 10.1038/s12276-023-01046-5
2. Noncoding RNAs as Promising Diagnostic Biomarkers and Therapeutic Targets in Intestinal Fibrosis of Crohn's Disease: The Path From Bench to Bedside.
   Zhou LY, Lin SN, Rieder F, Chen MH, et al · · 2021 · cited 11× · PMID 33324986 · DOI 10.1093/ibd/izaa321
3. Long non-coding RNAs in ferroptosis, pyroptosis and necroptosis: from functions to clinical implications in cancer therapy.
   Huang K, Yu L, Lu D, Zhu Z, et al · · 2024 · cited 10× · PMID 39267831 · DOI 10.3389/fonc.2024.1437698
4. Exploring the translational landscape of the long noncoding RNA transcriptome in acute respiratory distress syndrome: it is a long way to the top.
   Belmonte T, Rodríguez-Muñoz C, Ferruelo A, Exojo-Ramírez SM, et al · · 2024 · cited 4× · PMID 38925793 · DOI 10.1183/16000617.0013-2024
5. Potential Intersections between lncRNA, Vascular Cognitive Impairment, and Immunization Strategies: Insights and Future Directions.
   Fan Y, Xiao B, Zhang M. · · 2024 · cited 3× · PMID 38543885 · DOI 10.3390/vaccines12030251