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NCT04186780

Effects of Lentinula Edodes Bars on Dyslipidemia and Oxidative Stress in Cholesterol Individuals: Randomized Study

Completed NA Results posted Last updated 27 October 2020
What this trial tests

NA trial testing Shiitake cereal bar in Dyslipidemias in 68 participants. Completed in 20 September 2019.

Timeline
26 September 2018
Primary endpoint
1 December 2018
20 September 2019

Quick facts

Lead sponsorUniversity of Sorocaba
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingtriple
Primary purposetreatment
Enrollment68
Start date26 September 2018
Primary completion1 December 2018
Estimated completion20 September 2019
Sites1 location across Brazil

Drugs / interventions tested

Conditions studied

Sponsor

University of Sorocaba

Who can join

Adults 20 to 65, any sex, with Dyslipidemias. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Total Cholesterol Levels Primary · Assessed at 0, 33 and 66 days. Days 0 and 66 reported.

Total cholesterol It was analyzed by commercial kits, according to the manufacturer's specifications. The volunteers' Total Cholesterol and Triglyceride levels were analyzed at Time 0 (when starting the study), at Time 33 (after 33 days) and at Time 66 (after 66 days).

Total Cholesterol T0
GroupValue95% CI
Placebo Group222.8± 33.1
Intervention Group228.2± 45.4
Total Cholesterol T66
GroupValue95% CI
Placebo Group226.7± 41.3
Intervention Group229.6± 38.2
Oxidative Stress: Catalase Secondary · Assessed at 0, 33 and 66 days. Days 0 and 66 reported.

The activity of the catalase enzyme was based on the decomposition of H2O2, monitored at 240 nm. A constant of variation or coefficient of variation (κ) per minute helped in the expression of catalase enzyme (κ/min). The Catalase enzyme has the responsibility to break down hydrogen peroxide. Higher scores mean a better outcome. Were analyzed at Time 0 (when starting the study), at Time 33 (after 33 days) and at Time 66 (after 66 days).

Catalase T0
GroupValue95% CI
Placebo Group3.5± 1.1
Intervention Group3.1± 0.8
Catalase T66
GroupValue95% CI
Placebo Group3.7± 1.6
Intervention Group3.7± 2.0
Oxidative Stress: Reduced Glutathione (GSH) Secondary · Assessed at 0, 33 and 66 days. Days 0 and 66 reported.

Reduced Glutathione was analyzed in the volunteers' samples at Time 0 (at the beginning of the study), at Time 33 (after 33 days) and at Time 66 (after 66 days).

GSH T0
GroupValue95% CI
Placebo Group0.4± 0.1
Intervention Group0.4± 0.2
GSH T66
GroupValue95% CI
Placebo Group0.6± 0.2
Intervention Group0.5± 0.2
Oxidative Stress: Thiobarbituric Acid Reactive Substances (TBARS) Secondary · Assessed at 0, 33 and 66 days. Days 0 and 66 reported.

Reactive Substances to Thiobarbituric Acid were analyzed in the volunteers' samples at Time 0 (at the beginning of the study), at Time 33 (after 33 days) and at Time 66 (after 66 days).

TBARS T0
GroupValue95% CI
Placebo Group13.9± 2.3
Intervention Group14.6± 2.1
TBARS T66
GroupValue95% CI
Placebo Group14.7± 2.0
Intervention Group13.9± 2.9
Triglycerides Levels Primary · Assessed at 0, 33 and 66 days. Days 0 and 66 reported.

The triglycerides levels It was analyzed by commercial kits, according to the manufacturer's specifications. The volunteers' Total Cholesterol and Triglyceride levels were analyzed at Time 0 (when starting the study), at Time 33 (after 33 days) and at Time 66 (after 66 days).

Triglycerides T0
GroupValue95% CI
Placebo Group144.5± 70.2
Intervention Group178.9± 170.1
Triglycerides T66
GroupValue95% CI
Placebo Group207.7± 202.7
Intervention Group160.7± 94.7

Adverse events — posted to ClinicalTrials.gov

Time frame: 15 days. Reporting threshold: 5%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Intervention Group
Serious: 0/36 (0%)
Deaths: 0/36
Placebo Group
Serious: 0/32 (0%)
Deaths: 0/32
Other adverse events (1 terms — click to expand)

ReactionSystemIntervention GroupPlacebo Group
Allergic ReactionSkin and subcutaneous tissue disorders

Data from ClinicalTrials.gov NCT04186780 adverse events section.

Sponsor's own description

The daily change in population routine has stimulated the development of health beneficial value added products. Lentinula edodes (Shiitake) is a mushroom with high protein concentration, low lipid content, rich in fiber, minerals, vitamins, antioxidant compounds, β-glucans and chitosan. This is clinical study phase II, randomized, double-blind for analyze in the effect of eating Shiitake bars on cholesterolemia and oxidative stress levels in individuals with borderline cholesterol. Individuals with at least one of the following biochemical markers (total cholesterol, LDL or triglycerides) at the borderline level were recruited through online questionnaire. Individuals (n = 68) were randomly allocated to two groups (Group I - Shiitake free bar (n = 32); Group II - Shiitake bar (n = 36). Each individual underwent blood collection at 0, 33 and 66 days, and received an unidentified opaque bag containing the bars.Biochemical analyzes (triglycerides, total cholesterol, LDL, HDL and glucose) and oxidative stress markers (Catalase, GSH and TBARS) were performed on samples of individuals.

Publications & conference data

2 peer-reviewed publications reference this trial (live from Europe PMC):

  1. β-Glucan Metabolic and Immunomodulatory Properties and Potential for Clinical Application.
    Murphy EJ, Rezoagli E, Major I, Rowan NJ, et al · · 2020 · cited 121× · PMID 33322069 · DOI 10.3390/jof6040356
  2. Narrative Review: Bioactive Potential of Various Mushrooms as the Treasure of Versatile Therapeutic Natural Product.
    Chopra H, Mishra AK, Baig AA, Mohanta TK, et al · · 2021 · cited 33× · PMID 34575766 · DOI 10.3390/jof7090728

Verify or expand the search:

Other recruiting trials for Dyslipidemias

Currently open trials in the same condition.

Other University of Sorocaba trials

Trials by the same sponsor.

Verify against primary sources

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