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NCT04060277

Triplex Vaccine in Preventing CMV Infection in Patients Undergoing Hematopoietic Stem Cell Transplantation

Active, enrolled Phase 2 Results posted Last updated 14 November 2025
What this trial tests

Phase 2 trial testing Letermovir in Accelerated Phase Chronic Myelogenous Leukemia, BCR-ABL1 Positive in 7 participants. Participants enrolled and being followed up; not accepting new ones.

Timeline
27 November 2019
Primary endpoint
6 June 2023
7 April 2030

Quick facts

Lead sponsorCity of Hope Medical Center
PhasePhase 2
StatusActive, enrolled
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingdouble
Primary purposesupportive care
Enrollment7
Start date27 November 2019
Primary completion6 June 2023
Estimated completion7 April 2030
Sites1 location across United States

Drugs / interventions tested

Conditions studied

Sponsor

City of Hope Medical Center

Who can join

18 and older, any sex, with Accelerated Phase Chronic Myelogenous Leukemia, BCR-ABL1 Positive or Acute Lymphoblastic Leukemia. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Number of Participants With Cytomegalovirus (CMV) Events Primary · From Day 100 to Day 180 post-hematopoietic stem cell transplant (HCT)

Cytomegalovirus (CMV) event was defined as CMV reactivation (DNAemia \>625 IU/ml by qPCR), viremia treated by antivirals, or detection of CMV by tissue histology (end-organ disease), from Day 100 post-HCT to Day 180 post-HCT. Trial participants were quantitatively monitored for viremia, by plasma qPCR test once every two weeks (+/- 5d) from Day 100 to Day 180. CMV monitoring used standard qPCR clinical laboratory methods to evaluate CMV viral load and possible vaccine failure.

GroupValue95% CI
Arm I (Letermovir, Triplex)0
Arm II (Letermovir, Placebo)0
Non-Relapse Mortality at Day 180 Post-Transplant Secondary · From time of transplant (Day 0) to Day 365

Non-relapse mortality (NRM) was defined as death without recurrent or progressive disease after transplant. NRM was estimated with the use of cumulative incidence curves, with relapse viewed as a competing risk.

GroupValue95% CI
Arm I (Letermovir, Triplex)0NA – NA
Arm II (Letermovir, Placebo)0NA – NA
Number of Participants With Severe (Grade 3-4) Acute Graft Versus Host Disease (GVHD) at Day 100 Secondary · From time of transplant (Day 0) to Day 100

Acute GVHD was assessed and graded according to the Keystone Consensus grading system (Przepiorka, D., et al., 1994 Consensus Conference on Acute GVHD Grading).

GroupValue95% CI
Arm I (Letermovir, Triplex)0
Arm II (Letermovir, Placebo)0
Number of Grade 3-4 Adverse Events Secondary · From time of transplant (Day 0) to Day 365

Adverse events probably or definitely related to the vaccination and modified vaccinia Ankara vector persistence were evaluated by NCI Common Terminology Criteria for Adverse Events v5.0.

GroupValue95% CI
Arm I (Letermovir, Triplex)0
Arm II (Letermovir, Placebo)0
Duration of Viremia Secondary · From time of transplant (Day 0) to Day 200

Duration of viremia was defined as the number of days from CMV qPCR \>625 IU/mL to serum viral clearance (no detectable CMV DNA in serial blood samples).

GroupValue95% CI
Arm I (Letermovir, Triplex)NANA – NA
Arm II (Letermovir, Placebo)NANA – NA
Number of Patients With Recurrence of CMV Viremia Secondary · From time of transplant (Day 0) to Day 200

CMV viremia was defined CMV qPCR \>625 IU/mL from samples collected within the past 7 days.

GroupValue95% CI
Arm I (Letermovir, Triplex)0
Arm II (Letermovir, Placebo)0
Days From Transplant to ANC Engraftment Secondary · From time of transplant to date of ANC engraftment, up to Day 365.

Date of ANC engraftment was defined as the first date of ANC of ≥ 0.5 x 109/L achieved for 3 consecutive lab tests on 3 different days.

GroupValue95% CI
Arm I (Letermovir, Triplex)1714 – 18
Arm II (Letermovir, Placebo)1614 – 17
Cumulative Incidence of Acute GVHD Secondary · From time of transplant (Day 0) to Day 100

Acute graft versus host disease is graded according to the 1994 Keystone Consensus Grading. The first day of acute GVHD onset was used to calculate the cumulative incidence. Relapse/death prior to onset was considered competing events.

GroupValue95% CI
Arm I (Letermovir, Triplex)255 – 100
Arm II (Letermovir, Placebo)0NA – NA
Cumulative Incidence of Chronic GVHD at Day 365 Secondary · From time of transplant (Day 0) to Day 365

Cumulative incidence of Chronic GVHD (cGVHD) was estimated with the use of cumulative incidence curves. The first day of cGVHD onset was used to calculate the cumulative incidence. Relapse/death prior to onset was considered competing events.

GroupValue95% CI
Arm I (Letermovir, Triplex)337 – 100
Arm II (Letermovir, Placebo)0NA – NA
Cumulative Incidence of Relapse at Day 365 Secondary · From time of transplant (Day 0) to Day 365

Cumulative incidence of relapse was estimated with the use of cumulative incidence curves, with death viewed as a competing risk.

GroupValue95% CI
Arm I (Letermovir, Triplex)0NA – NA
Arm II (Letermovir, Placebo)0NA – NA
Number Of Participants Died at Day 365 Secondary · From time of transplant (Day 0) to Day 365

Number of participants, who died due to all causes, at Day 365 post-transplant.

GroupValue95% CI
Arm I (Letermovir, Triplex)0
Arm II (Letermovir, Placebo)0
Overall Survival at Day 365 Secondary · From time of transplant (Day 0) to Day 365

Estimates was calculated using the Kaplan-Meier method, Greenwood formula was used to calculate SE, and loglog transformation method was used to construct 95% confidence intervals. Each patient's vital status was monitored per clinical standard operating procedure. For this endpoint failure is defined as death (from any cause). Patients not experiencing a death event was censored at his/her date of last contact.

GroupValue95% CI
Arm I (Letermovir, Triplex)100100 – 100
Arm II (Letermovir, Placebo)100100 – 100

Adverse events — posted to ClinicalTrials.gov

Time frame: Adverse events and all-cause mortality were monitored/assessed at Day 100, 114, 128, 140, 180, 210-240, 270 and 365.. Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Arm I (Letermovir, Triplex)
Serious: 1/4 (25%)
Deaths: 0/4
Arm II (Letermovir, Placebo)
Serious: 0/3 (0%)
Deaths: 0/3

Serious adverse events (1 terms)

ReactionSystemArm I (Letermovir, Triplex)Arm II (Letermovir, Placebo)
FeverGeneral disorders
Other adverse events (51 terms — click to expand)

ReactionSystemArm I (Letermovir, Triplex)Arm II (Letermovir, Placebo)
Rash maculo-papularSkin and subcutaneous tissue disorders
NauseaGastrointestinal disorders
White blood cell decreasedInvestigations
AnemiaBlood and lymphatic system disorders
Dry eyeEye disorders
VomitingGastrointestinal disorders
ChillsGeneral disorders
Neutrophil count decreasedInvestigations
Platelet count decreasedInvestigations
HypoalbuminemiaMetabolism and nutrition disorders
HypomagnesemiaMetabolism and nutrition disorders
HeadacheNervous system disorders
HypertensionVascular disorders
EosinophiliaBlood and lymphatic system disorders
Sinus tachycardiaCardiac disorders
TinnitusEar and labyrinth disorders
Eye painEye disorders
Abdominal painGastrointestinal disorders
BloatingGastrointestinal disorders
GastritisGastrointestinal disorders
Gastroesophageal reflux diseaseGastrointestinal disorders
Oral painGastrointestinal disorders
ToothacheGastrointestinal disorders
Edema faceGeneral disorders
Edema limbsGeneral disorders
FatigueGeneral disorders
FeverGeneral disorders
Injection site reactionGeneral disorders
motion sicknessGeneral disorders
PainGeneral disorders
BacteremiaInfections and infestations
Upper respiratory infectionInfections and infestations
Alanine aminotransferase increasedInvestigations
Alkaline phosphatase increasedInvestigations
Aspartate aminotransferase increasedInvestigations
Lymphocyte count decreasedInvestigations
HyperglycemiaMetabolism and nutrition disorders
HypertriglyceridemiaMetabolism and nutrition disorders
HypocalcemiaMetabolism and nutrition disorders
HyponatremiaMetabolism and nutrition disorders

Most-reported serious reactions: Fever.

Data from ClinicalTrials.gov NCT04060277 adverse events section.

Sponsor's own description

This phase II trial studies how well Triplex vaccine works in preventing cytomegalovirus (CMV) infection in patients undergoing a hematopoietic stem cell transplantation. CMV is a virus that may be carried for life and does not cause illness in most healthy individuals. However, in people whose immune systems are lowered (such as those undergoing stem cell transplantation), CMV can reproduce and cause disease and even death. The Triplex vaccine is made up of 3 small pieces of CMV deoxyribonucleic acid (DNA) (the chemical form of genes) placed into a weakened virus called modified vaccinia Ankara (MVA) that may help produce immunity (the ability to recognize and respond to an infection) and reduce the risk of developing complications related to CMV infection.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Cancer vaccines as promising immuno-therapeutics: platforms and current progress.
    Liu J, Fu M, Wang M, Wan D, et al · · 2022 · cited 497× · PMID 35303904 · DOI 10.1186/s13045-022-01247-x
  2. Progress and Challenges in the Prevention, Diagnosis, and Management of Cytomegalovirus Infection in Transplantation.
    Limaye AP, Babu TM, Boeckh M. · · 2020 · cited 87× · PMID 33115722 · DOI 10.1128/cmr.00043-19
  3. Therapeutic cancer vaccines: From biological mechanisms and engineering to ongoing clinical trials.
    Sobhani N, Scaggiante B, Morris R, Chai D, et al · · 2022 · cited 57× · PMID 35759856 · DOI 10.1016/j.ctrv.2022.102429
  4. Therapeutic vaccines for herpesviruses.
    Cohen JI. · · 2024 · cited 24× · PMID 38690731 · DOI 10.1172/jci179483
  5. Lessons from Acquired Natural Immunity and Clinical Trials to Inform Next-Generation Human Cytomegalovirus Vaccine Development.
    Hu X, Wang HY, Otero CE, Jenks JA, et al · · 2022 · cited 23× · PMID 35704747 · DOI 10.1146/annurev-virology-100220-010653
  6. Human cytomegalovirus: pathogenesis, prevention, and treatment.
    Shang Z, Li X. · · 2024 · cited 19× · PMID 39585514 · DOI 10.1186/s43556-024-00226-7
  7. Haploidentical Stem Cell Transplantation for Acute Myeloid Leukemia: Current Therapies, Challenges and Future Prospective.
    Chang YJ, Zhao XY, Huang XJ. · · 2021 · cited 15× · PMID 34778077 · DOI 10.3389/fonc.2021.758512
  8. Recent Findings on Therapeutic Cancer Vaccines: An Updated Review.
    Sheikhlary S, Lopez DH, Moghimi S, Sun B. · · 2024 · cited 8× · PMID 38672519 · DOI 10.3390/biom14040503

Verify or expand the search:

Other trials of Letermovir

Trials testing the same drug.

Other recruiting trials for Accelerated Phase Chronic Myelogenous Leukemia, BCR-ABL1 Positive

Currently open trials in the same condition.

Other City of Hope Medical Center trials

Trials by the same sponsor.

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Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT04060277.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing