NCT04047459

Investigation of Cancer Cell Extravasation Through mRNA Analysis of Organ-specific Endothelial Cells and Microfluidics

Completed Last updated 6 August 2019

What this trial tests

trial testing Tissue samples collection and cells isolation in Breast Cancer Metastatic in 31 participants. Completed in 31 May 2018.

Timeline

6 June 2016

Primary endpoint
31 May 2018

31 May 2018

Quick facts

Lead sponsor	I.R.C.C.S Ospedale Galeazzi-Sant'Ambrogio
Status	Completed
Study type	OBSERVATIONAL
Enrollment	31
Start date	6 June 2016
Primary completion	31 May 2018
Estimated completion	31 May 2018
Sites	1 location across Italy

Drugs / interventions tested

• Tissue samples collection and cells isolation

Conditions studied

• Breast Cancer Metastatic — all drugs for Breast Cancer Metastatic →

Sponsor

I.R.C.C.S Ospedale Galeazzi-Sant'Ambrogio — full company profile →

Who can join

Adults 18 to 65, any sex, with Breast Cancer Metastatic. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

The project aims at unraveling the role of organ-specific endothelia mediating the preferential metastasisation of breast cancer cells to bone by using a multi-faceted approach, integrating microfluidics and transcriptomic profiling. Based on a recently study published by the investigators \[Jeon et al., PNAS 2015\], it can be hypothesized that phenotypic differences at the level of organ-specific endothelial cells are able to drive the preferential extravasation of breast cancer cells to specific sites. Hence, the transcriptional profile of primary organ-specific endothelial cells derived from healthy patients (i.e. non-affected by breast cancer) will be analyzed to identify phenotypic differences between organ-specific populations of endothelial cells. These analyses will allow to identify potential target genes involved in the organ-specific extravasation of cancer cells (i.e. genes differentially expressed by endothelia of preferential and non-preferential metastasisation sites). The selected genes will be silenced and the effect of gene silencing will be evaluated through microfluidic in vitro organ-specific 3D models designed to study cancer cell extravasation.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

1. Differential angiogenesis of bone and muscle endothelium in aging and inflammatory processes.
   Arrigoni C, Ostano P, Bersini S, Crippa M, et al · · 2023 · cited 10× · PMID 36721025 · DOI 10.1038/s42003-023-04515-9