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NCT04010695

Validation of Diagnostics to Identify Glucose-6-phosphate Dehydrogenase Activity in the US

Completed NA Results posted Last updated 10 May 2022
What this trial tests

NA trial testing SD Biosensor G6PD Analyzer in G6PD Deficiency in 250 participants. Completed in 19 September 2019.

Timeline
13 May 2019
Primary endpoint
19 September 2019
19 September 2019

Quick facts

Lead sponsorPATH
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationna
Designsingle group
Maskingnone
Primary purposediagnostic
Enrollment250
Start date13 May 2019
Primary completion19 September 2019
Estimated completion19 September 2019
Sites1 location across United States

Drugs / interventions tested

Conditions studied

Sponsor

PATH — full company profile →

Who can join

Adults 18 to 65, any sex, with G6PD Deficiency. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Sensitivity of SD Biosensor POC G6PD Test for Identifying G6PD Deficient Individuals Primary · All samples were collected on study day 1

For the purposes of this study, an individual was considered G6PD deficient if they tested positive in the Pointe Scientific assay. A true positive was defined as a participant with ≤ 30% of normal G6PD activity in circulating venous blood determined by the Pointe Scientific test. Diagnostic sensitivity of the SD Biosensor G6PD test is defined as the percentage of participants who tested positive for G6PD deficiency on the reference test who were identified by the test assay as positive, calculated as: Number of participants who were both test and true positive / (Number of participants who

Venous Blood
GroupValue95% CI
G6PD Diagnostic Testing100.059.0 – 100.0
Capillary Blood
GroupValue95% CI
G6PD Diagnostic Testing100.059.0 – 100.0
Sensitivity of SD Biosensor POC G6PD Test for Identifying Women With Intermediate G6PD Activity Primary · All samples were collected on study day 1

To investigate the performance of the SD Biosensor G6PD test to distinguish females with intermediate G6PD activity from females with normal activity, assay sensitivity was determined at a G6PD activity threshold of 70%. A true positive for intermediate G6PD activity in females was defined as G6PD activity between 30 and 70% of normal in circulating venous blood as determined by the Pointe Scientific test. Sensitivity of the SD Biosensor POC G6PD test is the percentage of women who tested positive for intermediate G6PD activity on the reference test who were identified by the test assay as po

Venous Blood
GroupValue95% CI
G6PD Diagnostic Testing100.039.8 – 100.0
Capillary Blood
GroupValue95% CI
G6PD Diagnostic Testing75.019.4 – 99.4
Specificity of SD Biosensor POC G6PD Test for Identifying G6PD Deficient Individuals Primary · All samples were collected on study day 1

For the purposes of this study, an individual was considered G6PD deficient if they tested positive by the Pointe Scientific assay. A true negative was defined as \> 30% of normal G6PD activity in circulating venous blood as determined by the Pointe Scientific test. Specificity is the percentage of participants who tested negative for G6PD deficiency on the reference test who were identified as negative using the test assay, calculated as: Number of participants who were both test and true negative / (Number of participants who were test and true negative + Number of participants who were te

Venous Blood
GroupValue95% CI
G6PD Diagnostic Testing94.990.8 – 97.5
Capillary Blood
GroupValue95% CI
G6PD Diagnostic Testing98.595.8 – 99.7
Specificity of SD Biosensor G6PD Test for Identifying Women With Intermediate G6PD Activity Primary · All samples were collected on study day 1

To investigate the performance of the SD Biosensor G6PD test to distinguish females with intermediate G6PD activity from females with normal activity, the specificity was determined at a G6PD activity threshold of 70%. A true negative for intermediate G6PD activity in females was defined as G6PD activity \> 70% of normal in circulating venous blood as determined by the Pointe Scientific test. Specificity is the percentage of participants who tested negative for G6PD deficiency on the reference test who were identified as negative using the test assay, calculated as: Number of participants wh

Venous Blood
GroupValue95% CI
G6PD Diagnostic Testing83.776.2 – 89.6
Capillary Blood
GroupValue95% CI
G6PD Diagnostic Testing92.486.4 – 96.3
Accuracy Between the SD Biosensor POC G6PD Test Assay and the Pointe Scientific Test Kit Secondary · All samples were collected on study day 1

Accuracy between the SD Biosensor POC G6PD test assay and the Pointe Scientific G6PD reference assay was calculated as the percent agreement between both G6PD tests, ie, the percentage of participants with the same diagnosis according to both tests (either deficient, intermediate, or normal G6PD levels). Accuracy of the SD Biosensor G6PD test was calculated from both venous and capillary blood samples.

Venous blood
GroupValue95% CI
G6PD Diagnostic Testing85.780.1 – 90.2
Capillary blood
GroupValue95% CI
G6PD Diagnostic Testing93.989.7 – 96.7
Accuracy Between the SD Biosensor POC G6PD Test Measure of Hemoglobin and the Reference Hemoglobin Test Secondary · All samples were collected on study day 1

Accuracy between the SD Biosensor POC G6PD test measure of hemoglobin and the hemoglobin reference assay was calculated as the percentage agreement between both tests, ie, the percentage of participants with the same diagnosis according to both tests (either non/mild anemia, moderate anemia or severe anemia).

Venous blood
GroupValue95% CI
G6PD Diagnostic Testing97.594.3 – 99.1
Capillary blood
GroupValue95% CI
G6PD Diagnostic Testing89.684.7 – 93.4
Median G6PD Values Measured by the SD Biosensor G6PD Test for Venous and Capillary Blood Samples Secondary · All samples were collected on study day 1
Venous blood
GroupValue95% CI
G6PD Diagnostic Testing7.66.8 – 8.5
Capillary blood
GroupValue95% CI
G6PD Diagnostic Testing6.86.1 – 7.5

Sponsor's own description

The primary objective of this study is to assess the accuracy of the SD Biosensor STANDARD G6PD Analyzer in measuring G6PD activity when used by trained health care workers.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

  1. Reference and point-of-care testing for G6PD deficiency: Blood disorder interference, contrived specimens, and fingerstick equivalence and precision.
    Pal S, Myburgh J, Bansil P, Hann A, et al · · 2021 · cited 18× · PMID 34543346 · DOI 10.1371/journal.pone.0257560

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Other recruiting trials for G6PD Deficiency

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