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NCT03962686

Molecular Mechanisms and Carotid Atherosclerosis

Completed Last updated 3 August 2022
What this trial tests

trial testing statin Oral Tablet plus PCSK9i in Diabetes Mellitus, Type 2 in 76 participants. Completed in 1 January 2018.

Timeline
1 January 2015
Primary endpoint
1 January 2017
1 January 2018

Quick facts

Lead sponsorUniversity of Campania Luigi Vanvitelli
StatusCompleted
Study typeOBSERVATIONAL
Enrollment76
Start date1 January 2015
Primary completion1 January 2017
Estimated completion1 January 2018
Sites1 location across Italy

Drugs / interventions tested

Conditions studied

Sponsor

University of Campania Luigi Vanvitelli

Who can join

Adults 18 to 75, any sex, with Diabetes Mellitus, Type 2 or Atherosclerosis of Artery. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

The role of methylase system and Proprotein convertase subtilisin/kexin type 9 (PCSK9) in the accelerated atherosclerotic progression of diabetic patients is unclear. Authors will evaluate methylase activity and PCSK9 in carotid plaques of asymptomatic diabetic and non diabetic patients, as well as the effect of statin added to PCSK9 inhibitors (PCSK9i) therapy vs. statin alone in diabetic plaques. Plaques will be obtained from 43 type 2 diabetic and 30 non diabetic patients undergoing carotid endarterectomy. Diabetic patients will receive statin therapy (n 23) or statin plus PCSK9i (140 mg of evolocumab; n 20) or placebo (n 23) for 4 months before scheduled endarterectomy. Plaques will be analyzed for macrophages (CD68), T-cells (CD3), inflammatory cells (HLADR), methylase activity, nuclear factor (NF)-KB, tumor necrosis factor (TNF)-alpha, nitrotyrosine, matrix metalloproteinase (MMP) and collagen content (immunohistochemistry and enzyme- linked immunosorbent assay. Authors' study hypothesis is that methylase and PCSK9 over-activity will be associated with enhanced inflammatory reaction and NF-KB expression in diabetic plaques. Secondly, the inhibition of methylase activity in atherosclerotic lesions of diabetic patients by metformin plus SLGT2i might be associated with morphological and compositional characteristics of a potential stable plaque phenotype, possibly by down regulating NF-KB-mediated inflammatory pathways.

Publications & conference data

3 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Sodium-glucose co-transporter2 expression and inflammatory activity in diabetic atherosclerotic plaques: Effects of sodium-glucose co-transporter2 inhibitor treatment.
    D'Onofrio N, Sardu C, Trotta MC, Scisciola L, et al · · 2021 · cited 87× · PMID 34500107 · DOI 10.1016/j.molmet.2021.101337
  2. Endothelial mechanobiology in atherosclerosis.
    Wang X, Shen Y, Shang M, Liu X, et al · · 2023 · cited 79× · PMID 37163659 · DOI 10.1093/cvr/cvad076
  3. Advances and challenges of targeting epicardial adipose tissue (EAT) and perivascular adipose tissue (PVAT).
    Liu D, Chen W, Guo Z, Gao Q, et al · · 2025 · cited 1× · PMID 40760703 · DOI 10.1186/s12933-025-02763-z

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Other recruiting trials for Diabetes Mellitus, Type 2

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Data sources for this page

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