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NCT03961191

DNA Methylation for Screening Uterine Cervical Lesions: A Case-control Study

Status unknown Last updated 23 May 2019
What this trial tests

trial testing DNA methylation in DNA Methylation in 300 participants. Status unknown.

Timeline
22 May 2019
Primary endpoint
22 May 2020
22 May 2020

Quick facts

Lead sponsorLei Li
StatusStatus unknown
Study typeOBSERVATIONAL
Enrollment300
Start date22 May 2019
Primary completion22 May 2020
Estimated completion22 May 2020
Sites1 location across China

Drugs / interventions tested

Conditions studied

Sponsor

Lei Li — full company profile →

Who can join

18 and older, female only, with DNA Methylation or Uterine Cervical Cancer. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

The primary objective of this study is to compare the testing of DNA methylation, high-risk HPV subtypes, and cytology with the definite histological results in a case-control study, so as to determine the accuracy of DNA methylation in the screening of uterine cervical lesions. This study will include 300 patients with definite histological results, with 100 of cervical inflammation or low grade squamous intraepithelial lesions (LSIL), 100 of high grade squamous intraepithelial lesions (HSIL), and 100 of uterine cervical cancer. All the cervical specimens of cytology collected in the clinical settings will be utilized for the testing of DNA methylation, high-risk HPV subtypes and thin prep liquid-based cytology test (TCT). The sensitivity, specificity, positive predictive value and negative predictive value were calculated based on the known histological results. The differences of DNA methylation with high-risk human papillomavirus (HPV) and TCT will also be analyzed. The testing of DNA methylation will be performed with the methylation-specific polymerase chain reaction (PCR). The TCT and HPV testing will be performed with the Roche kits.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

  1. DNA methylation for cervical cancer screening: a training set in China.
    Kong L, Wang L, Wang Z, Xiao X, et al · · 2020 · cited 32× · PMID 32576279 · DOI 10.1186/s13148-020-00885-7

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