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NCT03941730

Estradiol in Treating Patients With ER Beta Positive, Triple Negative Locally Advanced or Metastatic Breast Cancer

Active, enrolled Phase 2 Results posted Last updated 5 March 2026
What this trial tests

Phase 2 trial testing Biopsy in Advanced Triple-Negative Breast Carcinoma in 8 participants. Participants enrolled and being followed up; not accepting new ones.

Timeline
28 August 2019
Primary endpoint
29 July 2024
31 December 2026

Quick facts

Lead sponsorMayo Clinic
PhasePhase 2
StatusActive, enrolled
Study typeINTERVENTIONAL
Allocationna
Designsingle group
Maskingnone
Primary purposetreatment
Enrollment8
Start date28 August 2019
Primary completion29 July 2024
Estimated completion31 December 2026
Sites9 locations across United States

Drugs / interventions tested

Conditions studied

Sponsor

Mayo Clinic

Who can join

18 and older, female only, with Advanced Triple-Negative Breast Carcinoma or Anatomic Stage III Breast Cancer AJCC v8. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Clinical Benefit Rate Primary · 173 days

A patient is said to have derived clinical benefit rate at the 6 month time point if the patient's disease meets the Response Evaluation Criteria in Solid Tumors (RECIST) for complete response (CR), partial response (PR), or stable disease (SD) for \> 6 months following initiation of treatment. The 6 month clinical benefit rate is the percentage of patients who are found to meet the criteria for clinical benefit at least 6 months among all the patients who have started estradiol treatment. As the number of patients with discordant ERbeta findings are expected to be small, a 90% exact binomial

GroupValue95% CI
Treatment (Estradiol)0
Tumor Response Rate Among Those Patients With Measurable Disease Secondary · 173 days

The tumor response rate is defined as the 100% time the number of patients with a CR or PR (as defined by the RECIST criteria) on 2 consecutive evaluations at least 8 weeks apart divided by the total number of eligible patients who began study treatment. A 90% binomial confidence interval is constructed for the true response rate.

GroupValue95% CI
Treatment (Estradiol)0
Progression Free Survival (PFS) Distribution Secondary · 173 days

PFS is defined as the time from registration to the first of the following events: local, regional, or distant recurrence, second primary disease of death due to any cause. The distribution of PFS times will be estimated using the method of Kaplan-Meier.

GroupValue95% CI
Treatment (Estradiol)5019 – 173
Overall Survival (OS) Distribution Secondary · 18 months

OS is defined as the time from registration to death due to any cause. The distribution of survival times are estimated using the method of Kaplan-Meier.

GroupValue95% CI
Treatment (Estradiol)3.81 – 17.7

Adverse events — posted to ClinicalTrials.gov

Time frame: Adverse Events followed for 6 months and mortality followed for 18 months. Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Treatment (Estradiol)
Serious: 3/8 (38%)
Deaths: 7/8

Serious adverse events (5 terms)

ReactionSystemTreatment (Estradiol)
Hepatic failureHepatobiliary disorders
Aspartate aminotransferase increasedInvestigations
Blood bilirubin increasedInvestigations
Vaginal hemorrhageReproductive system and breast disorders
Pleural effusionRespiratory, thoracic and mediastinal disorders
Other adverse events (42 terms — click to expand)

ReactionSystemTreatment (Estradiol)
NauseaGastrointestinal disorders
FatigueGeneral disorders
CoughRespiratory, thoracic and mediastinal disorders
HyponatremiaMetabolism and nutrition disorders
Vaginal hemorrhageReproductive system and breast disorders
DyspneaRespiratory, thoracic and mediastinal disorders
AnemiaBlood and lymphatic system disorders
Abdominal painGastrointestinal disorders
Edema limbsGeneral disorders
Neutrophil count decreasedInvestigations
Platelet count decreasedInvestigations
White blood cell decreasedInvestigations
AnorexiaMetabolism and nutrition disorders
Bone painMusculoskeletal and connective tissue disorders
Peripheral sensory neuropathyNervous system disorders
AnxietyPsychiatric disorders
Breast painReproductive system and breast disorders
Pleural effusionRespiratory, thoracic and mediastinal disorders
HypertensionVascular disorders
Lymph node painBlood and lymphatic system disorders
Paroxysmal atrial tachycardiaCardiac disorders
Blurred visionEye disorders
BloatingGastrointestinal disorders
ConstipationGastrointestinal disorders
DiarrheaGastrointestinal disorders
Gastroesophageal reflux diseaseGastrointestinal disorders
Non-cardiac chest painGeneral disorders
Alkaline phosphatase increasedInvestigations
HypercalcemiaMetabolism and nutrition disorders
HyperglycemiaMetabolism and nutrition disorders
Metabolism, nutrition disord - Oth specMetabolism and nutrition disorders
Back painMusculoskeletal and connective tissue disorders
OsteoporosisMusculoskeletal and connective tissue disorders
Pain in extremityMusculoskeletal and connective tissue disorders
HeadacheNervous system disorders
Renal calculiRenal and urinary disorders
Urinary frequencyRenal and urinary disorders
Urinary urgencyRenal and urinary disorders
DysmenorrheaReproductive system and breast disorders
Vaginal dischargeReproductive system and breast disorders

Most-reported serious reactions: Hepatic failure, Aspartate aminotransferase increased, Blood bilirubin increased, Vaginal hemorrhage, Pleural effusion.

Data from ClinicalTrials.gov NCT03941730 adverse events section.

Sponsor's own description

This phase II trial studies how well estradiol works in treating patients with estrogen receptor beta (ER beta) positive, triple negative breast cancer that has spread to nearby tissue or lymph nodes (locally advanced) or other places in the body (metastatic). Hormone receptors like ER beta allow the body to respond appropriately to hormones. Triple negative means that the breast cancer does not express other hormone receptors called ER alpha, progesterone, and HER2. In some people with triple negative breast cancer, ER beta is overexpressed. Tumor cells that overexpress ER beta grow slower in the laboratory and this growth is slowed in the presence of estrogen. Estradiol is a form of estrogen. This study may help doctors determine whether tumor cells that overexpress ER beta shrink in the presence of estradiol.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Recent advances in therapeutic strategies for triple-negative breast cancer.
    Li Y, Zhang H, Merkher Y, Chen L, et al · · 2022 · cited 643× · PMID 36038913 · DOI 10.1186/s13045-022-01341-0
  2. Estrogen Actions in Triple-Negative Breast Cancer.
    Treeck O, Schüler-Toprak S, Ortmann O. · · 2020 · cited 58× · PMID 33114740 · DOI 10.3390/cells9112358
  3. Estrogen Receptor Beta: The Promising Biomarker and Potential Target in Metastases.
    Božović A, Mandušić V, Todorović L, Krajnović M. · · 2021 · cited 47× · PMID 33562134 · DOI 10.3390/ijms22041656
  4. Insights into the Role of Estrogen Receptor β in Triple-Negative Breast Cancer.
    Sellitto A, D'Agostino Y, Alexandrova E, Lamberti J, et al · · 2020 · cited 34× · PMID 32516978 · DOI 10.3390/cancers12061477
  5. Estrogen receptor beta repurposes EZH2 to suppress oncogenic NFκB/p65 signaling in triple negative breast cancer.
    Aspros KGM, Carter JM, Hoskin TL, Suman VJ, et al · · 2022 · cited 15× · PMID 35177654 · DOI 10.1038/s41523-022-00387-0
  6. Estrogen Receptor Beta 1: A Potential Therapeutic Target for Female Triple Negative Breast Cancer.
    Dey P, Wang A, Ziegler Y, Kumar S, et al · · 2022 · cited 14× · PMID 36251879 · DOI 10.1210/endocr/bqac172
  7. The Potential of Hormonal Therapies for Treatment of Triple-Negative Breast Cancer.
    Kirkby M, Popatia AM, Lavoie JR, Wang L. · · 2023 · cited 12× · PMID 37835396 · DOI 10.3390/cancers15194702
  8. Carcinogenesis of Triple-Negative Breast Cancer and Sex Steroid Hormones.
    Honma N, Matsuda Y, Mikami T. · · 2021 · cited 10× · PMID 34070471 · DOI 10.3390/cancers13112588

Verify or expand the search:

Other trials of Biopsy

Trials testing the same drug.

Other recruiting trials for Advanced Triple-Negative Breast Carcinoma

Currently open trials in the same condition.

Other Mayo Clinic trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT03941730.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing