Last reviewed · How we verify

NCT03883113

Efficacy of MVA-NP+M1 in the Influenza H3N2 Human Challenge Model

Completed Phase 2 Results posted Last updated 18 March 2021
What this trial tests

Phase 2 trial testing MVA-NP+M1 in Influenza in 145 participants. Completed in 17 April 2020.

Timeline
3 June 2019
Primary endpoint
16 December 2019
17 April 2020

Quick facts

Lead sponsorBarinthus Biotherapeutics
PhasePhase 2
StatusCompleted
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingquadruple
Primary purposeprevention
Enrollment145
Start date3 June 2019
Primary completion16 December 2019
Estimated completion17 April 2020
Sites1 location across Belgium

Drugs / interventions tested

Conditions studied

Sponsor

Barinthus Biotherapeutics — full company profile →

Who can join

Adults 18 to 55, any sex, with Influenza. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Degree of Nasopharyngeal Viral Shedding as Determined by Quantitative Polymerase Chain Reaction qPCR Primary · Throughout 9 days (Day2, Day3, Day4, Day5, Day6, Day7, Day8, Day9, Day10) after viral Inoculation (Day1) of the challenge phase. Nasal swabs taken twice a day (b.i.d) at least 8 hours apart.

Measure of nasopharyngeal viral shedding during challenge; recorded as viral area under curve (vAUC) as determined by quantitative real time polymerase chain reaction (qRT-PCR). vAUC is calculated by plotting the log viral particles number/ml for each time point against time and is using the trapezoidal rule.

GroupValue95% CI
MVA-NP+M1 (ITT)649.7552.7 – 746.7
Placebo (ITT)726.1604.0 – 848.2
MVA-NP+M1 (PP)646.5548.3 – 744.7
Placebo (PP)726.1604.0 – 848.2
MVA-NP+M1 (Challenge)649.7552.7 – 746.7
Placebo (Challenge)726.1604.0 – 848.2
Number and Percentage of Virologically Confirmed Influenza-Like Illness Secondary · 9 days from day 2 to day 10

Incidence (frequency tabulation) of laboratory-confirmed influenza-like illness compared between vaccine and placebo arms Virologically confirmed influenza-like illness (ILI) is defined as having respiratory or flu-like symptom occurring on two consecutive days, along with a positive qPCR or qCulture result.

Virologically confirmed Influenza-like Illness
GroupValue95% CI
MVA-NP+M1 (ITT)43
Placebo (ITT)29
No Virologically Confirmed Influenza-like Illness
GroupValue95% CI
MVA-NP+M1 (ITT)28
Placebo (ITT)18
Percentage of Participants With Attack Rate of Challenge Agent (qRT-PCR) Secondary · 9 days from day 2 to day 10

The attack rate is defined as the percentage of inoculated participants with at least two consecutive positive swabs as determined by qRT-PCR within the timespan of two consecutive days

qPCR-confirmed influenza
GroupValue95% CI
MVA-NP+M1 (ITT)90.180.7 – 95.9
Placebo (ITT)97.988.7 – 99.9
No qPCR influenza
GroupValue95% CI
MVA-NP+M1 (ITT)9.94.1 – 19.3
Placebo (ITT)2.10.1 – 11.3
Percentage of Participants With Quantitative Culture Attack Rate of Challenge Agent (qCulture) Secondary · 9 days from day 2 to day 10

The attack rate is defined as the percentage of inoculated participants with at least two consecutive positive swabs as determined by qCulture within the timespan of two consecutive days

qCulture confirmed influenza
GroupValue95% CI
MVA-NP+M1 (ITT)77.566.0 – 86.5
Placebo (ITT)85.171.7 – 93.8
No qCulture-confirmed influenza
GroupValue95% CI
MVA-NP+M1 (ITT)22.513.5 – 34.0
Placebo (ITT)14.96.2 – 28.3
Time to Start of Viral Shedding (qPCR) From Virus Inoculation Secondary · 9 days from day 2 to day 10

The Time to Start of Viral Shedding (qPCR) is calculated as (datetime of first of two positive swabs (qPCR) within 2 consecutive days - challenge datetime)/(60\*60)

GroupValue95% CI
MVA-NP+M1 (ITT)24.4024.30 – 24.50
Placebo (ITT)24.3024.20 – 24.50
Time to Start of Viral Shedding (qCulture) From Virus Inoculation Secondary · 9 days from day 2 to day 10

Time to Start of Viral Shedding (qCulture) is calculated as (datetime of first of two positive swabs (qCulture) within 2 consecutive days - challenge datetime)/(60\*60)

GroupValue95% CI
MVA-NP+M1 (ITT)35.935.60 – 48.00
Placebo (ITT)47.624.70 – 48.50
Peak Viral Shedding (qPCR) After the Virus Inoculation Secondary · 9 days from Day 2 to Day 10

This is measured by the highest viral load concentration by qPCR

GroupValue95% CI
MVA-NP+M1 (ITT) Successful Attack5.8765.430 – 6.322
Placebo (ITT) Successful Attack6.0545.521 – 6.587
Peak Viral Shedding (qCulture) After Virus Inoculation Secondary · 9 days from day 2 to day 10

This is measured by the highest viral load concentration by qCulture.

GroupValue95% CI
MVA-NP+M1 (ITT) Successful Attack4.0733.683 – 4.463
Placebo (ITT) Successful Attack4.0693.442 – 4.695
Time to Peak of Viral Shedding (qPCR) From the Viral Inoculation Secondary · 9 days from day 2 to day 10

This is calculated as (datetime of highest viral load concentration (qPCR) - challenge datetime)/(60\*60)

GroupValue95% CI
MVA-NP+M1 (ITT)72.2060.00 – 96.00
Placebo (ITT)107.9072.20 – 119.80
Time to Peak of Viral Shedding (qCulture) From the Viral Inoculation Secondary · 9 days from day 2 to day 10

This is calculated as (datetime of highest viral load concentration (qCulture) - challenge datetime)/(60\*60)

GroupValue95% CI
MVA-NP+M1 (ITT)83.4060.10 – 96.30
Placebo (ITT)83.8060.10 – 96.40
Duration of Viral Shedding (qPCR) After the Virus Inoculation Secondary · 9 days from day2 to day10

It is calculated as (datetime of first negative swab (qPCR) following the last positive swab (qPCR) - datetime of first positive of two positive swabs (qPCR) within 2 consecutive days)/(60\*60)

GroupValue95% CI
MVA-NP+M1 (ITT) Successful Attack170.80157.08 – 184.52
Placebo (ITT) Successful Attack172.47156.99 – 187.94
Duration of Viral Shedding (qCulture) After the Virus Inoculation Secondary · 9 days from day 2 to day 10

It is calculated as (datetime of first negative swab (qCulture) following the last positive swab (qCulture) - datetime of first positive of two positive swabs (qCulture) within 2 consecutive days)/(60\*60)

GroupValue95% CI
MVA-NP+M1 (ITT) Successful Attack118.19105.07 – 131.31
Placebo (ITT) Successful Attack121.78106.33 – 137.23

Adverse events — posted to ClinicalTrials.gov

Time frame: Any time during the study from the first administration of any study drug until the last study-related activity (the outpatient visit at 27 days ±3 days following the challenge or 6 months following vaccination ±14 days, whichever is longer). Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

MVA-NP+M1 (Vaccination Phase)
Serious: 1/87 (1%)
Deaths: 0/87
Saline Placebo (Vaccination Phase)
Serious: 0/58 (0%)
Deaths: 0/58
MVA-NP+M1 (Challenge Phase)
Serious: 0/71 (0%)
Deaths: 0/71
Saline Placebo (Challenge Phase)
Serious: 1/47 (2%)
Deaths: 0/47

Serious adverse events (2 terms)

ReactionSystemMVA-NP+M1 (Vaccination Pha…Saline Placebo (Vaccinatio…MVA-NP+M1 (Challenge Phase)Saline Placebo (Challenge …
Foetal deathPregnancy, puerperium and perinatal conditions
Acute PsychosisPsychiatric disorders
Other adverse events (54 terms — click to expand)

ReactionSystemMVA-NP+M1 (Vaccination Pha…Saline Placebo (Vaccinatio…MVA-NP+M1 (Challenge Phase)Saline Placebo (Challenge …
NasopharyngitisInfections and infestations
Back PainMusculoskeletal and connective tissue disorders
DiarrhoeaGastrointestinal disorders
HeadacheNervous system disorders
Throat IrritationRespiratory, thoracic and mediastinal disorders
EpistaxisRespiratory, thoracic and mediastinal disorders
UrticariaSkin and subcutaneous tissue disorders
Skin IrritationSkin and subcutaneous tissue disorders
GastroenteritisInfections and infestations
Oral HerpesInfections and infestations
RhinorrhoeaRespiratory, thoracic and mediastinal disorders
Abdominal PainGastrointestinal disorders
Neck PainMusculoskeletal and connective tissue disorders
Eye IrritationEye disorders
Ear DiscomfortEar and labyrinth disorders
DysmenorrhoeaReproductive system and breast disorders
ConjunctivitisInfections and infestations
RhinitisInfections and infestations
Upper Respiratory Tract InfectionInfections and infestations
SneezingRespiratory, thoracic and mediastinal disorders
CoughRespiratory, thoracic and mediastinal disorders
Dry ThroatRespiratory, thoracic and mediastinal disorders
Nasal CongestionRespiratory, thoracic and mediastinal disorders
Abdominal DiscomfortGastrointestinal disorders
Abdominal Pain UpperGastrointestinal disorders
ConstipationGastrointestinal disorders
Dry MouthGastrointestinal disorders
NauseaGastrointestinal disorders
RegurgitationGastrointestinal disorders
ToothacheGastrointestinal disorders
VomitingGastrointestinal disorders
DysphoniaRespiratory, thoracic and mediastinal disorders
Dizziness PosturalNervous system disorders
PresyncopeNervous system disorders
EczemaSkin and subcutaneous tissue disorders
Dry SkinSkin and subcutaneous tissue disorders
BursitisMusculoskeletal and connective tissue disorders
Flank PainMusculoskeletal and connective tissue disorders
Musculoskeletal PainMusculoskeletal and connective tissue disorders
Musculoskeletal StiffnessMusculoskeletal and connective tissue disorders

Most-reported serious reactions: Foetal death, Acute Psychosis.

Data from ClinicalTrials.gov NCT03883113 adverse events section.

Sponsor's own description

A Phase 2, single center, randomized, double blind study evaluating the safety, efficacy, and immunogenicity of MVA NP+M1 in the H3N2 human influenza challenge model; on healthy adult volunteers.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Challenges of Making Effective Influenza Vaccines.
    Gouma S, Anderson EM, Hensley SE. · · 2020 · cited 71× · PMID 32392457 · DOI 10.1146/annurev-virology-010320-044746
  2. Progress towards the Development of a Universal Influenza Vaccine.
    Wang WC, Sayedahmed EE, Sambhara S, Mittal SK. · · 2022 · cited 49× · PMID 36016306 · DOI 10.3390/v14081684
  3. T Cell Immunity against Influenza: The Long Way from Animal Models Towards a Real-Life Universal Flu Vaccine.
    Schmidt A, Lapuente D. · · 2021 · cited 30× · PMID 33525620 · DOI 10.3390/v13020199
  4. A Decade in Review: A Systematic Review of Universal Influenza Vaccines in Clinical Trials during the 2010 Decade.
    Corder BN, Bullard BL, Poland GA, Weaver EA. · · 2020 · cited 26× · PMID 33092070 · DOI 10.3390/v12101186
  5. Development of Modified Vaccinia Virus Ankara-Based Vaccines: Advantages and Applications.
    Orlova OV, Glazkova DV, Bogoslovskaya EV, Shipulin GA, et al · · 2022 · cited 22× · PMID 36146594 · DOI 10.3390/vaccines10091516
  6. Randomized, Double-Blind, Reference-Controlled, Phase 2a Study Evaluating the Immunogenicity and Safety of OVX836, A Nucleoprotein-Based Influenza Vaccine.
    Leroux-Roels I, Waerlop G, Tourneur J, De Boever F, et al · · 2022 · cited 22× · PMID 35464450 · DOI 10.3389/fimmu.2022.852904
  7. A methylation clock model of mild SARS-CoV-2 infection provides insight into immune dysregulation.
    Mao W, Miller CM, Nair VD, Ge Y, et al · · 2023 · cited 17× · PMID 36919946 · DOI 10.15252/msb.202211361
  8. Phase 1 Randomized, Placebo-Controlled, Dose-Escalating Study to Evaluate OVX836, a Nucleoprotein-Based Influenza Vaccine: Intramuscular Results.
    Withanage K, De Coster I, Cools N, Viviani S, et al · · 2022 · cited 16× · PMID 34653245 · DOI 10.1093/infdis/jiab532

Verify or expand the search:

Other trials of MVA-NP+M1

Trials testing the same drug.

Other recruiting trials for Influenza

Currently open trials in the same condition.

Other Barinthus Biotherapeutics trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT03883113.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing