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NCT03612310

Developing Protocols for Modelling of Genetic Diseases Using Induced Pluripotent Stem Cells

Not yet recruiting Last updated 2 August 2018
What this trial tests

trial testing Skin biopsy/Urine Collection/Blood Sample Collection in Genetic Disease in 3,000 participants. Not yet recruiting.

Timeline
1 November 2018
Primary endpoint
1 July 2020
1 July 2028

Quick facts

Lead sponsorKevin Bruce
StatusNot yet recruiting
Study typeOBSERVATIONAL
Enrollment3,000
Start date1 November 2018
Primary completion1 July 2020
Estimated completion1 July 2028

Drugs / interventions tested

Conditions studied

Sponsor

Kevin Bruce

Who can join

Adults 1 to 120, any sex, with Genetic Disease. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

Recent advances have shown that cells from human blood, skin and urine samples can be reprogrammed to become stem cells. These are called induced Pluripotent Stem Cells (iPSCs) and share many characteristics with embryonic stem cells, including an unlimited capacity for proliferation and the potential to become any cell in the body. Beneficially, the use of iPSCs avoids the ethical difficulties which surround embryonic stem cells and allows generation of iPSC lines which are disease representative. For example, we could take skin samples from an individual diagnosed with Huntington's disease and their unaffected sibling and using this technology, generate iPSC lines from both individuals. Using these iPSCs, we could produce disease affected cell populations from the affected and unaffected individuals, use these cells to research why specific cell populations are affected by disease and test new treatments to combat disease progression, essentially producing a 'disease in a dish'. This is just one example of many for which this technology could be applied. We can also utilise gene-editing techniques to generate isogenic controls or insert disease related mutations to assess disease phenotype. Although generation of iPSC lines has been robustly proven across multiple disease backgrounds, many aspects of their downstream use still remain to be determined. Particularly, robust protocols for directing iPSCs towards cell fates such as neurons or blood cells must be developed to fully realise application of iPSCs in disease modelling and drug screening. This study involves the collection of human blood, skin or urine samples from subjects bearing a range of genetic diseases alongside those from individuals who have not been diagnosed with a disease, as controls. These samples will be used to generate iPSC lines for development of differentiation and disease phenotyping protocols.

Publications & conference data

No peer-reviewed publications indexed yet for this trial.

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Data sources for this page

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