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NCT03370172

A Study of BAX 888 in Male Adults With Severe Hemophilia A

Completed Phase 1, PHASE2 Results posted Last updated 11 September 2025
What this trial tests

Phase 1, PHASE2 trial testing BAX 888 in Hemophilia A in 4 participants. Completed in 9 July 2024.

Timeline
27 February 2018
Primary endpoint
9 July 2024
9 July 2024

Quick facts

Lead sponsorBaxalta now part of Shire
PhasePhase 1, PHASE2
StatusCompleted
Study typeINTERVENTIONAL
Allocationnon randomized
Designsequential
Maskingnone
Primary purposeprevention
Enrollment4
Start date27 February 2018
Primary completion9 July 2024
Estimated completion9 July 2024
Sites26 locations across France, Austria, Germany, Hungary, United States, Spain

Drugs / interventions tested

Conditions studied

Sponsor

Baxalta now part of Shire — full company profile →

Who can join

Adults 18 to 75, male only, with Hemophilia A. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Number of Participants With BAX 888-Related Adverse Events (AEs) Primary · From first dose up to end of the study (approximately 6 years)

An AE is defined as any untoward medical occurrence in a participant administered an investigational product (IP) that does not necessarily have a causal relationship with the treatment. A Serious adverse event (SAE) is an AE resulting in any of the following outcomes: death; life-threatening event; required or prolonged inpatient hospitalization; persistent or significant disability/incapacity; congenital anomaly. AEs include both serious and non-serious adverse events including development of FVIII inhibitory antibodies, clinically significant changes in standard laboratory parameters, physi

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg2
Cohort 2: BAX 888 6.0*10^12 cp/kg2
Change From Baseline in Circulating Plasma FVIII Activity Level Secondary · Baseline, up to Month 60

Change from baseline in circulating plasma FVIII activity level, based on one-stage clotting assay was assessed.

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg11.40± 1.131
Cohort 2: BAX 888 6.0*10^12 cp/kg248.30± NA
Number of Participants With Clinically Significant Change From Baseline in Circulating Plasma FVIII Antigen Level Secondary · Baseline, up to Month 60

Change from baseline in circulating plasma FVIII antigen (protein) levels were to be assessed and number of participants with clinically significant change as determined by the principal investigator (PI) were reported.

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg2
Cohort 2: BAX 888 6.0*10^12 cp/kg0
Annualized Bleed Rate (ABR) Secondary · Up to approximately 6 years 4 months

ABR in comparison to before gene transfer will be assessed. A bleed is defined as subjective or objective evidence of bleeding which may or may not require treatment with FVIII. ABR was calculated as (number of bleeding episodes/observed treatment period in days)\*365.25.

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1.0± 1.41
Cohort 2: BAX 888 6.0*10^12 cp/kg0.5± 0.71
Percentage of Participants With a Reduction in Consumption of Exogenous FVIII Secondary · Up to approximately 6 years 4 months

The reduction in consumption of exogenous FVIII was assessed by comparing the amount of exogenous FVIII taken at earliest time point available (prior to BAX 888 infusion) with the amount taken at the last post-infusion timepoint available, during the study. Percentage of participants with reduction in consumption of exogenous FVIII are reported.

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg0.0
Cohort 2: BAX 888 6.0*10^12 cp/kg0.0
Number of Participants Who Developed Inhibitory Antibodies to FVIII Secondary · Up to approximately 6 years 4 months

Participants were assessed to check if they developed inhibitory antibodies to FVIII.

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg0
Cohort 2: BAX 888 6.0*10^12 cp/kg0
Number of Participants Who Developed Total Binding Antibodies to FVIII Secondary · Up to approximately 6 years 4 months

Participants were assessed to check if they developed total binding antibodies to FVIII (Immunoglobulin G \[IgG\], Immunoglobulin M \[IgM\]).

GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg0
Cohort 2: BAX 888 6.0*10^12 cp/kg0
Number of Participants With Humoral and Cell-Mediated Immune Response to AAV8 and FVIII Proteins Secondary · Up to approximately 6 years 4 months

The humoral (antibody-mediated) and cell-mediated immune response to adeno-associated virus (AAV8) (the vector) and FVIII proteins, was assessed. Humoral Immune Response: It is indicated by presence of specific antibodies. The anti-AAV8 binding antibodies, IgG or IgM were measured by the enzyme-linked immunosorbent assay (ELISA) method. Neutralizing antibodies were measured by a cell-based luminescent assay. Cell-mediated Immune response: The AAV8 and FVIII specific cell mediated immunity was assessed using validated interferon-γ (IFN-γ) enzyme-linked immunosorbent spot (ELISpot) assays. This

Humoral: Binding Ab to AAV8 IgG Titer
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg2
Cohort 2: BAX 888 6.0*10^12 cp/kg1
Humoral: Neutralising Ab to AAV8 Titer
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg2
Cohort 2: BAX 888 6.0*10^12 cp/kg1
Cell-Mediated: AAV8 Peptide Pool 1 Mean
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg0
Cohort 2: BAX 888 6.0*10^12 cp/kg1
Cell-Mediated: AAV8 Peptide Pool 2 Mean
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1
Cohort 2: BAX 888 6.0*10^12 cp/kg1
Cell-Mediated: AAV8 Peptide Pool 3 Mean
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1
Cohort 2: BAX 888 6.0*10^12 cp/kg1
Cell-Mediated: FVIII Peptide Pool 1 Mean
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg0
Cohort 2: BAX 888 6.0*10^12 cp/kg0
Cell-Mediated: FVIII Peptide Pool 2 Mean
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1
Cohort 2: BAX 888 6.0*10^12 cp/kg1
Surveillance of AAV8 Genome Shedding Secondary · Blood: Day 1, weekly at Clinic Visits between Weeks 1-15, and at Months 4 and 5; Saliva, Semen, and Stool: Day 1 and Week 1; Urine: Day 1 and Weeks 1,2,3

Surveillance of AAV8 genome shedding in blood, saliva, semen, stool and urine until two consecutive negative results were assessed.

Blood: Day 1
GroupValue95% CI
Cohort 2: BAX 888 6.0*10^12 cp/kg3684434.0± NA
Blood: Week 1
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg3634.0± NA
Cohort 2: BAX 888 6.0*10^12 cp/kg18608.0± 664.68
Blood: Week 2
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg2076.0± 295.57
Cohort 2: BAX 888 6.0*10^12 cp/kg10670.0± 3900.40
Blood: Week 3
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg2069.0± 741.05
Cohort 2: BAX 888 6.0*10^12 cp/kg9845.0± 6488.41
Blood: Week 4
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1098.0± NA
Cohort 2: BAX 888 6.0*10^12 cp/kg9033.5± 4978.74
Blood: Week 5
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1483.0± 1097.43
Cohort 2: BAX 888 6.0*10^12 cp/kg7884.0± 288.50
Blood: Week 6
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg904.0± 335.17
Cohort 2: BAX 888 6.0*10^12 cp/kg5372.5± 441.94
Blood: Week 7
GroupValue95% CI
Cohort 1: BAX 888 2.0*10^12 cp/kg1004.0± 656.20
Cohort 2: BAX 888 6.0*10^12 cp/kg6808.0± 1121.47

Adverse events — posted to ClinicalTrials.gov

Time frame: All-cause mortality: Up to approximately 6 years 4 months; SAEs and Other (Non-Serious) AEs: From first dose up to end of the study (approximately 6 years). Reporting threshold: 5%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Cohort 1: BAX 888 2.0*10^12 cp/kg
Serious: 0/2 (0%)
Deaths: 0/2
Cohort 2: BAX 888 6.0*10^12 cp/kg
Serious: 1/2 (50%)
Deaths: 0/2

Serious adverse events (1 terms)

ReactionSystemCohort 1: BAX 888 2.0*10^1…Cohort 2: BAX 888 6.0*10^1…
HypophosphataemiaMetabolism and nutrition disorders
Other adverse events (46 terms — click to expand)

ReactionSystemCohort 1: BAX 888 2.0*10^1…Cohort 2: BAX 888 6.0*10^1…
HypertransaminasaemiaHepatobiliary disorders
NasopharyngitisInfections and infestations
SARS-CoV-2 test positiveInvestigations
Abdominal pain upperGastrointestinal disorders
ArthralgiaMusculoskeletal and connective tissue disorders
ArthritisMusculoskeletal and connective tissue disorders
AstheniaGeneral disorders
Back painMusculoskeletal and connective tissue disorders
COVID-19Infections and infestations
DermatitisSkin and subcutaneous tissue disorders
Dermatitis acneiformSkin and subcutaneous tissue disorders
DermatosisSkin and subcutaneous tissue disorders
Dry eyeEye disorders
DyspnoeaRespiratory, thoracic and mediastinal disorders
ErythemaSkin and subcutaneous tissue disorders
FallInjury, poisoning and procedural complications
GastroenteritisInfections and infestations
HeadacheNervous system disorders
HyperphagiaMetabolism and nutrition disorders
HypertensionVascular disorders
HypophosphataemiaMetabolism and nutrition disorders
Increased appetiteMetabolism and nutrition disorders
Influenza like illnessGeneral disorders
Initial insomniaPsychiatric disorders
IrritabilityPsychiatric disorders
Ligament sprainInjury, poisoning and procedural complications
MigraineNervous system disorders
Musculoskeletal chest painMusculoskeletal and connective tissue disorders
Musculoskeletal discomfortMusculoskeletal and connective tissue disorders
NauseaGastrointestinal disorders
Night sweatsSkin and subcutaneous tissue disorders
Pain in extremityMusculoskeletal and connective tissue disorders
ParaesthesiaNervous system disorders
PharyngitisInfections and infestations
RashSkin and subcutaneous tissue disorders
RhinitisInfections and infestations
RhinorrhoeaRespiratory, thoracic and mediastinal disorders
SomnolenceNervous system disorders
SynovitisMusculoskeletal and connective tissue disorders
TachycardiaCardiac disorders

Most-reported serious reactions: Hypophosphataemia.

Data from ClinicalTrials.gov NCT03370172 adverse events section.

Sponsor's own description

The main aim of this study is to check if there are side effects from BAX 888 and to determine the dose of BAX 888 for treating severe hemophilia A in male adults. Participants will receive one infusion with BAX 888 at the hemophilia treatment center. During the study, participants will visit their study clinic multiple times.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Viral vector platforms within the gene therapy landscape.
    Bulcha JT, Wang Y, Ma H, Tai PWL, et al · · 2021 · cited 899× · PMID 33558455 · DOI 10.1038/s41392-021-00487-6
  2. Update on clinical gene therapy for hemophilia.
    Perrin GQ, Herzog RW, Markusic DM. · · 2019 · cited 128× · PMID 30559260 · DOI 10.1182/blood-2018-07-820720
  3. Adeno-Associated Virus Gene Therapy for Hemophilia.
    Samelson-Jones BJ, George LA. · · 2023 · cited 97× · PMID 36103998 · DOI 10.1146/annurev-med-043021-033013
  4. Testing preexisting antibodies prior to AAV gene transfer therapy: rationale, lessons and future considerations.
    Mendell JR, Connolly AM, Lehman KJ, Griffin DA, et al · · 2022 · cited 87× · PMID 35356756 · DOI 10.1016/j.omtm.2022.02.011
  5. A Molecular Revolution in the Treatment of Hemophilia.
    Butterfield JSS, Hege KM, Herzog RW, Kaczmarek R. · · 2020 · cited 76× · PMID 31843450 · DOI 10.1016/j.ymthe.2019.11.006
  6. Gene therapy for hemophilia.
    Nathwani AC. · · 2022 · cited 74× · PMID 36485127 · DOI 10.1182/hematology.2022000388
  7. Emerging Immunogenicity and Genotoxicity Considerations of Adeno-Associated Virus Vector Gene Therapy for Hemophilia.
    Monahan PE, Négrier C, Tarantino M, Valentino LA, et al · · 2021 · cited 62× · PMID 34199563 · DOI 10.3390/jcm10112471
  8. How to discuss gene therapy for haemophilia? A patient and physician perspective.
    Miesbach W, O'Mahony B, Key NS, Makris M. · · 2019 · cited 60× · PMID 31115117 · DOI 10.1111/hae.13769

Verify or expand the search:

Other recruiting trials for Hemophilia A

Currently open trials in the same condition.

Other Baxalta now part of Shire trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT03370172.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing