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NCT03246529: GENESIS

A Phase III, Safety, Tolerability and Efficacy of Combination Treatment of BL-8040 and Granulocyte Colony Stimulating Factor (G-CSF) as Compared to Placebo and G-CSF for the Mobilization of Hematopoietic Stem Cells for Autologous Transplantation in Subjects With Multiple Myeloma (MM)

Active, enrolled Phase 3 Results posted Last updated 15 January 2026
What this trial tests

Phase 3 trial testing BL-8040 1.25 mg/kg + G-CSF in Multiple Myeloma in 180 participants. Participants enrolled and being followed up; not accepting new ones.

Timeline
23 March 2018
Primary endpoint
22 December 2020
30 September 2029

Quick facts

Lead sponsorBioLineRx, Ltd.
PhasePhase 3
StatusActive, enrolled
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingtriple
Primary purposetreatment
Enrollment180
Start date23 March 2018
Primary completion22 December 2020
Estimated completion30 September 2029
Sites18 locations across Italy, Germany, Hungary, United States, Spain

Drugs / interventions tested

Conditions studied

Sponsor

BioLineRx, Ltd. — full company profile →

Who can join

Adults 18 to 78, any sex, with Multiple Myeloma. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Percentage of Subjects Mobilizing ≥6 × 10^6 CD34+ Cells/kg With up to 2 Apheresis Sessions Primary · From first day of study treatment (G-CSF) until day of second apheresis which was planned to occur on Day 6

Percentage of subjects mobilizing ≥6 × 10\^6 CD34+ cells/kg with up to 2 apheresis sessions in preparation for autologous hematopoetic cell transplantation (auto-HCT) after treatment with G-CSF + single administration of BL-8040/placebo. Based on central laboratory data.

Central Lab
GroupValue95% CI
BL-8040 + G-CSF Part 270.0
Placebo + G-CSF Part 214.3
Local Lab
GroupValue95% CI
BL-8040 + G-CSF Part 191.7
BL-8040 + G-CSF Part 292.5
Placebo + G-CSF Part 226.2
Percentage of Subjects Mobilizing ≥2 × 10^6 CD34+ Cells/kg in 1 Apheresis Session Secondary · From first day of study treatment (G-CSF) until day of first apheresis which was planned to occur on Day 5

Percentage of subjects mobilizing ≥2 × 10\^6 CD34+ cells/kg in 1 apheresis session after treatment with G-CSF + single administration of BL-8040/ placebo.

Central Lab
GroupValue95% CI
BL-8040 + G-CSF Part 287.5
Placebo + G-CSF Part 247.6
Local Lab
GroupValue95% CI
BL-8040 + G-CSF Part 1100
BL-8040 + G-CSF Part 296.3
Placebo + G-CSF Part 264.3
Percentage of Subjects Mobilizing ≥6 × 10^6 CD34+ Cells/kg in 1 Apheresis Session Secondary · From first day of study treatment (G-CSF) until day of first apheresis which was planned to occur on Day 5

Percentage of subjects mobilizing ≥6 × 10\^6 CD34+ cells/kg in 1 apheresis session after treatment with G-CSF + single administration of BL-8040/ placebo.

Central Lab
GroupValue95% CI
BL-8040 + G-CSF Part 267.5
Placebo + G-CSF Part 24.8
Local Lab
GroupValue95% CI
BL-8040 + G-CSF Part 175
BL-8040 + G-CSF Part 288.8
Placebo + G-CSF Part 29.5
Time to Neutrophil Engraftment, After Auto-HCT Secondary · End of engraftment period, which was defined as 29 days post transplantation

Time to neutrophil engraftment after auto-HCT, where engraftment was defined as absolute neutrophil count (ANC) ≥0.5 × 10\^9/L for 3 days or ≥1.0 × 10\^9/L for 1 day following the conditioning regimen associated nadir.

GroupValue95% CI
BL-8040 + G-CSF Part 11211 – 12
BL-8040 + G-CSF Part 21211 – 12
Placebo + G-CSF Part 21211 – 12
Time to Platelet Engraftment, After Auto-HCT Secondary · End of engraftment period, which was defined as 29 days post transplantation

Time to platelet engraftment, after auto-HCT, where engraftment was defined as the first of 3 consecutive measurements of platelet count ≥20 × 10\^9/L without platelet transfusion support for 7 days following the conditioning regimen associated nadir.

GroupValue95% CI
BL-8040 + G-CSF Part 11715.0 – 20.0
BL-8040 + G-CSF Part 21817 – 19
Placebo + G-CSF Part 21717 – 18
Subjects With Graft Durability at 100 Days Post Transplant/ Early Termination Secondary · Day 100 Post-Transplantation (± 7 days)

Subjects achieving graft durability were defined as meeting the following 2 criteria: * Platelet count ≥50 × 10\^9/L without transfusion for at least 2 weeks. * Hemoglobin level ≥10 g/dL with no erythropoietin support or transfusions for at least 1 month. This analysis was performed in part 2 of the study only.

GroupValue95% CI
BL-8040 + G-CSF Part 292.2
Placebo + G-CSF Part 291.9
Graft Durability at 6 Months Post Transplantation Secondary · 6 Months Post Transplantation

Comparability of the graft durability between the BL-8040 + G-CSF arm and the placebo + G-CSF arm at 6 months post transplantation

GroupValue95% CI
BL-8040 + G-CSF Part 280.5
Placebo + G-CSF Part 283.8
Graft Durability at 9 Months Post Transplantation Secondary · 9 Months Post Transplantation

Comparability of the graft durability between the BL-8040 + G-CSF arm and the placebo + G-CSF arm at 9 months post transplantation

GroupValue95% CI
BL-8040 + G-CSF Part 283.1
Placebo + G-CSF Part 281.1
Graft Durability at 12 Months Post Transplantation Secondary · 12 Month Post Transplantation

Comparability of the graft durability between the BL-8040 + G-CSF arm and the placebo + G-CSF arm at 12 months post transplantation

GroupValue95% CI
BL-8040 + G-CSF Part 281.8
Placebo + G-CSF Part 281.1

Adverse events — posted to ClinicalTrials.gov

Time frame: All Adverse Events (AEs) were collected after subjects signed the ICF and up to 30 days from the last dose of study therapy (G-CSF+BL-8040/Placebo).. Reporting threshold: 5%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

BL-8040 + G-CSF Part 1
Serious: 1/12 (8%)
Deaths: 0/12
BL-8040 + G-CSF (Part 2)
Serious: 7/80 (9%)
Deaths: 2/80
Placebo + G-CSF (Part 2)
Serious: 0/42 (0%)
Deaths: 1/42

Serious adverse events (9 terms)

ReactionSystemBL-8040 + G-CSF Part 1BL-8040 + G-CSF (Part 2)Placebo + G-CSF (Part 2)
Injection site reactionGeneral disorders
HypersensitivityImmune system disorders
BacteremiaInfections and infestations
Injection site cellulitisInfections and infestations
Platelet count decreasedInvestigations
Bladder neoplasmNeoplasms benign, malignant and unspecified (incl cysts and polyps)
HypoxiaRespiratory, thoracic and mediastinal disorders
HypokalaemiaGastrointestinal disorders
VomitingGastrointestinal disorders
Other adverse events (44 terms — click to expand)

ReactionSystemBL-8040 + G-CSF Part 1BL-8040 + G-CSF (Part 2)Placebo + G-CSF (Part 2)
Injection site painGeneral disorders
PruritusSkin and subcutaneous tissue disorders
Injection site erythemaGeneral disorders
Injection site pruritusGeneral disorders
Back painMusculoskeletal and connective tissue disorders
Bone painMusculoskeletal and connective tissue disorders
NauseaGastrointestinal disorders
HypokalaemiaMetabolism and nutrition disorders
UrticariaSkin and subcutaneous tissue disorders
Catheter site painGeneral disorders
ErythemaSkin and subcutaneous tissue disorders
ParaesthesiaNervous system disorders
RashSkin and subcutaneous tissue disorders
VomitingGastrointestinal disorders
HypersensitivityImmune system disorders
Dermatitis exfoliativeSkin and subcutaneous tissue disorders
ArthralgiaMusculoskeletal and connective tissue disorders
Injection site reactionGeneral disorders
HeadacheNervous system disorders
HypoaesthesiaNervous system disorders
Chest painGeneral disorders
HypomagnesaemiaMetabolism and nutrition disorders
FlushingVascular disorders
Musculoskeletal chest painMusculoskeletal and connective tissue disorders
Oedema peripheralGeneral disorders
HypocalcaemiaMetabolism and nutrition disorders
PyrexiaGeneral disorders
AnaemiaBlood and lymphatic system disorders
Spinal painMusculoskeletal and connective tissue disorders
Muscle spasmsMusculoskeletal and connective tissue disorders
Ear discomfortEar and labyrinth disorders
Auricular swellingEar and labyrinth disorders
Catheter site bruiseGeneral disorders
Injection site noduleGeneral disorders
Injection site urticariaGeneral disorders
ConstipationGastrointestinal disorders
DyspepsiaGastrointestinal disorders
Electrocardiogram QT prolongedInvestigations
White blood cell count increasedInvestigations
DysphoniaRespiratory, thoracic and mediastinal disorders

Most-reported serious reactions: Injection site reaction, Hypersensitivity, Bacteremia, Injection site cellulitis, Platelet count decreased, Bladder neoplasm, Hypoxia, Hypokalaemia.

Data from ClinicalTrials.gov NCT03246529 adverse events section.

Sponsor's own description

A total of 122 subjects were randomized into the study and investigated in the double-blind placebo-controlled setting to assess the efficacy and safety of G-CSF + BL-8040 as compared to G-CSF + placebo.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. The chemokines CXCL8 and CXCL12: molecular and functional properties, role in disease and efforts towards pharmacological intervention.
    Cambier S, Gouwy M, Proost P. · · 2023 · cited 387× · PMID 36725964 · DOI 10.1038/s41423-023-00974-6
  2. Targeting cytokine and chemokine signaling pathways for cancer therapy.
    Yi M, Li T, Niu M, Zhang H, et al · · 2024 · cited 264× · PMID 39034318 · DOI 10.1038/s41392-024-01868-3
  3. CXCR4 and CXCR7 Signaling Pathways: A Focus on the Cross-Talk Between Cancer Cells and Tumor Microenvironment.
    Santagata S, Ieranò C, Trotta AM, Capiluongo A, et al · · 2021 · cited 74× · PMID 33937018 · DOI 10.3389/fonc.2021.591386
  4. Motixafortide and G-CSF to mobilize hematopoietic stem cells for autologous transplantation in multiple myeloma: a randomized phase 3 trial.
    Crees ZD, Rettig MP, Jayasinghe RG, Stockerl-Goldstein K, et al · · 2023 · cited 53× · PMID 37069359 · DOI 10.1038/s41591-023-02273-z
  5. Development of pharmacological immunoregulatory anti-cancer therapeutics: current mechanistic studies and clinical opportunities.
    Yin N, Li X, Zhang X, Xue S, et al · · 2024 · cited 48× · PMID 38773064 · DOI 10.1038/s41392-024-01826-z
  6. Recent Advances in CXCL12/CXCR4 Antagonists and Nano-Based Drug Delivery Systems for Cancer Therapy.
    Zhao R, Liu J, Li Z, Zhang W, et al · · 2022 · cited 38× · PMID 35893797 · DOI 10.3390/pharmaceutics14081541
  7. Role of CXCR4 in the progression and therapy of acute leukaemia.
    Su L, Hu Z, Yang YG. · · 2021 · cited 38× · PMID 34050566 · DOI 10.1111/cpr.13076
  8. New genetic and epigenetic insights into the chemokine system: the latest discoveries aiding progression toward precision medicine.
    Xu H, Lin S, Zhou Z, Li D, et al · · 2023 · cited 37× · PMID 37198402 · DOI 10.1038/s41423-023-01032-x

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Other recruiting trials for Multiple Myeloma

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Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT03246529.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing