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NCT03216265

A Proof of Concept Clinical Study to Investigate the Effects of an Experimental Cosmetic Moisturiser on the Barrier Function of Human Skin on the Face and Forearm

Completed NA Results posted Last updated 18 April 2019
What this trial tests

NA trial testing Test product (Moisturising cream) in Skin Care in 69 participants. Completed in 24 April 2017.

Timeline
23 February 2017
Primary endpoint
19 April 2017
24 April 2017

Quick facts

Lead sponsorGlaxoSmithKline
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingsingle
Primary purposetreatment
Enrollment69
Start date23 February 2017
Primary completion19 April 2017
Estimated completion24 April 2017
Sites1 location across Germany

Drugs / interventions tested

Conditions studied

Sponsor

GlaxoSmithKline — full company profile →

Who can join

Adults 18 to 65, female only, with Skin Care. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Change From Baseline in Transepidermal Water Loss (TEWL) Measurements on Day 29, Test Product Treated Versus (vs.) Untreated Sites on the Forearm Primary · At Baseline and Day 29

TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 seconds (sec), to ensure that a stable value has been established. The first part of the measurement belongs to the equilibration phase. The values of the last 10 secs were averaged as the actual measurement values. An increase in TEWL values shows damage to the skin barrier function.

GroupValue95% CI
Test Product-0.59± 2.005
No Treatment0.79± 1.555
Change From Baseline in Transepidermal Water Loss (TEWL) Measurements on Day 29, Test Product Treated vs. Untreated Sites on the Face Secondary · At Baseline and Day 29

TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to ensure that a stable value has been established. The first part of the measurement belongs to the equilibration phase. The values of the last 10 secs were averaged as the actual measurement values. An increase in TEWL values shows damage to the skin barrier function.

GroupValue95% CI
Test Product-4.33± 3.729
No Treatment-3.18± 3.841
Change From Baseline in Corneometry on the Forearm and Face, Test Product Treated vs. Untreated Sites Secondary · At Baseline, Day 1 (30 minutes and 6 hours post study product application), Day 2, 15, and 29

Corneometry was used to measure moisture content of stratum corneum using corneometer. The measuring principle is based on changes in the capacitance of the measuring head, functioning as a condensator. Between the conductors of the probe an electrical field was built which allows the dielectricity of the stratum corneum to be measured. Because the dielectricity of the skin varies as a function of its water content. The Corneometer measurements was taken 5 times in total and then an average reading was calculated for each site and time point. An increase in corneometry values indicates an incr

Day 1 (30 mins), change from baseline forearm
GroupValue95% CI
Test Product14.29± 6.227
No Treatment-3.24± 5.556
Day 1 (6 hours), change from baseline, forearm
GroupValue95% CI
Test Product10.72± 5.174
No Treatment-0.34± 4.908
Day 2, change from baseline, forearm
GroupValue95% CI
Test Product8.65± 5.954
No Treatment1.48± 4.310
Day 15, change from baseline, forearm
GroupValue95% CI
Test Product12.02± 7.149
No Treatment3.06± 5.343
Day 29, change from baseline, forearm
GroupValue95% CI
Test Product11.85± 8.665
No Treatment4.50± 6.498
Day 1 (30 mins) change from baseline, face
GroupValue95% CI
Test Product25.12± 9.093
No Treatment-6.96± 7.647
Day 1 (6 hours ), change from baseline, face
GroupValue95% CI
Test Product13.94± 7.634
No Treatment-1.90± 7.152
Day 2, change from baseline, face
GroupValue95% CI
Test Product8.88± 8.501
No Treatment-0.45± 7.867
Change From Baseline in Transepidermal Water Loss (TEWL) on the Forearm and Face, Test Product Treated vs. Untreated Sites at Day 2 and 15 Secondary · At Baseline, Day 2, and 15

TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to ensure that a stable value has been established. The first part of the measurement belongs to the equilibration phase. The values of the last 10 secs were averaged as the actual measurement values. An increase in TEWL values shows damage to the skin barrier function.

Day 2, change from baseline, forearm
GroupValue95% CI
Test Product0.08± 1.766
No Treatment0.48± 1.458
Day 15, change from baseline, forearm
GroupValue95% CI
Test Product-0.23± 2.874
No Treatment1.07± 2.515
Day 2, change from baseline, face
GroupValue95% CI
Test Product-1.49± 2.624
No Treatment0.77± 3.969
Day 15, change from baseline, face
GroupValue95% CI
Test Product-4.79± 3.895
No Treatment-2.37± 4.354
Standardised Area Under Curve (AUC1-29) of Change From Baseline in Corneometry Over Treatment Period Secondary · Up to Day 29

Standardised AUC1-29 was calculated for each participant for change from baseline in corneometry on forearms and face over the treatment period; i.e. up to Day 29 using the trapezoidal rule and dividing by the number of days in the period. Corneometry was used to measure moisture content of stratum corneum using corneometer. The measuring principle is based on changes in the capacitance of the measuring head, functioning as a condensator. Between the conductors of the probe an electrical field was built which allows the dielectricity of the stratum corneum to be measured. Because the dielectri

AUC1-29, Forearm
GroupValue95% CI
Test Product10.87± 5.732
Positive Control10.20± 6.043
No Treatment2.88± 4.028
AUC1-29, Face
GroupValue95% CI
Test Product14.03± 7.061
Positive Control15.90± 10.719
No Treatment2.05± 9.149
Standardised Area Under Curve (AUC1-29) of Change From Baseline in Transepidermal Water Loss (TEWL) Over Treatment Period Secondary · Up to Day 29

Standardised AUC1-29 was calculated for each participant for change from baseline in TEWL on forearms and face over the treatment period; i.e. up to Day 29 using the trapezoidal rule and dividing by the number of days in the period. TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to ensure that a stable value has been established. The first part of the measurement belongs to the equilibration pha

AUC1-29, Forearm
GroupValue95% CI
Test Product-0.30± 1.849
Positive Control-0.42± 1.572
No Treatment0.82± 1.465
AUC1-29, Face
GroupValue95% CI
Test Product-3.95± 2.950
Positive Control-3.21± 4.465
No Treatment-1.67± 2.998
Change From Pre-challenge in Transepidermal Water Loss (TEWL) Measurements of D-Squame Discs Following 4, 8 and 12 Adhesive Discs Removal From Skin of Both Test Product Treated and Untreated Sites on the Forearm on Day 29 Secondary · On Day 29 (including Pre-challenge)

A series of D-Squame discs were gently smoothed over the designated D-Squame Areas by applying a uniform pressure for 5 secs with a stamp to ensure consistent adhesion to the skin. Each disc was pulled off the skin with one fluent and decisive movement. There were maximum of 12 D-Squame discs (in groups of 4) removed from each forearm repeatedly. TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to

Pre-challenge Day 29
GroupValue95% CI
Test Product6.96± 1.513
No Treatment8.33± 2.307
Change from pre-challenge after 4 discs
GroupValue95% CI
Test Product2.60± 1.504
No Treatment3.06± 1.995
Change from pre-challenge after 8 discs
GroupValue95% CI
Test Product3.35± 1.662
No Treatment4.94± 3.564
Change from pre-challenge after 12 discs
GroupValue95% CI
Test Product5.56± 2.780
No Treatment8.46± 6.392
Change From Pre-challenge in Transepidermal Water Loss (TEWL) Measurements of D-Squame Discs Following 3, 6 and 9 Adhesive Discs Removal From Skin of Both Test Product Treated and Untreated Sites on the Face on Day 29 Secondary · On Day 29 (including Pre-challenge)

A series of D-Squame discs were gently smoothed over the designated D-Squame Areas by applying a uniform pressure for 5 secs with a stamp to ensure consistent adhesion to the skin. Each disc was pulled off the skin with one fluent and decisive movement. There were maximum of 9 D-Squame discs (in groups of 3) removed from the face repeatedly. TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to ensu

Pre-challenge Day 29
GroupValue95% CI
Test Product13.10± 4.424
No Treatment14.63± 5.698
Change from pre-challenge after 3 Discs
GroupValue95% CI
Test Product4.58± 3.261
No Treatment6.45± 4.531
Change from pre-challenge after 6 Discs
GroupValue95% CI
Test Product11.34± 6.102
No Treatment16.66± 10.636
Change from pre-challenge after 9 Discs
GroupValue95% CI
Test Product20.68± 12.242
No Treatment27.26± 15.987
Protein Analysis (SquameScan) of D-Squame Discs (Total of 12 Adhesive Discs) From Skin of Both Test Product Treated and Untreated Sites on the Forearm on Day 29 Secondary · On Day 29

The protein content of each D-Squame disc was analysed using a SquameScan. SquameScan is the instrument used to indirectly measure the protein content extracted from the skin by D-squame tape strips. The determination was performed by measuring the optical absorption of the strip at about 850 nanometres (nm) (infrared light). The value displayed in % was proportionally related to the protein content. The protein content was analysed for each of the discs obtained the D-Squame stripping on the forearms and reported to 2 decimal places.

GroupValue95% CI
Test Product173.16± 44.816
No Treatment213.89± 29.648
Protein Analysis of D-Squame Discs (Total of 9 Adhesive Discs) From Skin of Both Test Product Treated and Untreated Sites on the Face on Day 29 Secondary · On Day 29

The protein content of each D-Squame disc was analysed using a SquameScan. SquameScan is the instrument used to indirectly measure the protein content extracted from the skin by D-squame tape strips. The determination was performed by measuring the optical absorption of the strip at about 850 nm (infrared light). The value displayed in % was proportionally related to the protein content. The protein content was analysed for each of the discs obtained the D-Squame stripping on the face and reported to 2 decimal places.

GroupValue95% CI
Test Product84.70± 23.413
No Treatment107.75± 20.999
Change From Baseline in Transepidermal Water Loss (TEWL) Measurements on Days 30, 31, 32, 33 and 34 in Test Product Treated vs. Untreated Sites on the Forearm and Face Secondary · At Baseline, Day 30, 31, 32, 33 and 34

TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to ensure that a stable value was established. The first part of the measurement belongs to the equilibration phase. The values of the last 10 secs were averaged as the actual measurement values. An increase in TEWL values shows damage to the skin barrier function.

Day 30, Change from baseline, forearm
GroupValue95% CI
Test Product-0.37± 2.351
No Treatment1.12± 2.348
Day 31, Change from baseline, forearm
GroupValue95% CI
Test Product-0.77± 1.986
No Treatment1.53± 2.804
Day 32,Change from baseline, forearm
GroupValue95% CI
Test Product-0.17± 2.322
No Treatment1.48± 2.110
Day 33,Change from baseline, forearm
GroupValue95% CI
Test Product-0.42± 1.902
No Treatment1.00± 1.961
Day 34, Change from baseline, forearm
GroupValue95% CI
Test Product-0.59± 1.889
No Treatment1.18± 1.927
Day 30, Change from baseline, face
GroupValue95% CI
Test Product-4.40± 4.145
No Treatment-3.18± 3.873
Day 31, Change from baseline, face
GroupValue95% CI
Test Product-4.26± 4.787
No Treatment-3.27± 4.112
Day 32, Change from baseline, face
GroupValue95% CI
Test Product-3.27± 5.264
No Treatment-3.05± 4.672
Change From Day 29 in Transepidermal Water Loss (TEWL) Measurements on Days 30, 31, 32, 33 and 34 in Test Product Treated vs. Untreated Sites on the Forearm and Face Secondary · At Day 30, 31, 32, 33, and 34

TEWL measuring principle is based on water vapour gradient determination between two pairs of sensors placed at different distances perpendicularly to the skin. The probe was held in place on the skin for one measurement, for approximately 40 secs, to ensure that a stable value was established. The first part of the measurement belongs to the equilibration phase. The values of the last 10 secs were averaged as the actual measurement values. An increase in TEWL values shows damage to the skin barrier function.

Day 30, change from Day 29, forearm
GroupValue95% CI
Test Product0.20± 1.970
No Treatment0.25± 1.696
Day 31, change from Day 29, forearm
GroupValue95% CI
Test Product-0.09± 1.494
No Treatment0.72± 1.885
Day 32, change from Day 29, forearm
GroupValue95% CI
Test Product0.45± 1.962
No Treatment0.67± 1.444
Day 33, change from Day 29, forearm
GroupValue95% CI
Test Product0.26± 1.673
No Treatment0.19± 1.737
Day 34, change from Day 29, forearm
GroupValue95% CI
Test Product0.03± 1.669
No Treatment0.37± 1.649
Day 30, change from Day 29, face
GroupValue95% CI
Test Product0.10± 3.341
No Treatment-0.05± 2.000
Day 31, change from Day 29, face
GroupValue95% CI
Test Product0.22± 3.807
No Treatment0.01± 2.833
Day 32, change from Day 29, face
GroupValue95% CI
Test Product1.17± 4.399
No Treatment0.22± 2.941

Adverse events — posted to ClinicalTrials.gov

Time frame: Approximately 42 days.. Reporting threshold: 1%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Test Product
Serious: 0/44 (0%)
Deaths: 0/44
Positive Control
Serious: 0/44 (0%)
Deaths: 0/44
No Treatment
Serious: 0/44 (0%)
Deaths: 0/44
Overall Participants
Serious: 0/66 (0%)
Deaths: 0/66
Other adverse events (25 terms — click to expand)

ReactionSystemTest ProductPositive ControlNo TreatmentOverall Participants
HEADACHENervous system disorders
NASOPHARYNGITISInfections and infestations
APPLICATION SITE PAPULESGeneral disorders
PYREXIAGeneral disorders
APPLICATION SITE ERYTHEMAGeneral disorders
APPLICATION SITE PRURITUSGeneral disorders
MIGRAINENervous system disorders
APPLICATION SITE SCABGeneral disorders
APPLICATION SITE BURNGeneral disorders
APPLICATION SITE DRYNESSGeneral disorders
APPLICATION SITE PAINGeneral disorders
APPLICATION SITE REACTIONGeneral disorders
INJURY ASSOCIATED WITH DEVICEGeneral disorders
CYSTITISInfections and infestations
EAR INFECTIONInfections and infestations
ABDOMINAL PAIN UPPERGastrointestinal disorders
NAUSEAGastrointestinal disorders
HAEMORRHOIDSGastrointestinal disorders
NEPHRITISRenal and urinary disorders
COUGHRespiratory, thoracic and mediastinal disorders
OROPHARYNGEAL PAINRespiratory, thoracic and mediastinal disorders
EYELID OEDEMAEye disorders
SEASONAL ALLERGYImmune system disorders
NEURODERMATITISSkin and subcutaneous tissue disorders
CONTUSIONInjury, poisoning and procedural complications

Data from ClinicalTrials.gov NCT03216265 adverse events section.

Sponsor's own description

The objective of this POC clinical study is to investigate the impact of the test product (Developmental Cosmetic Moisturising Cream) on skin barrier function and skin moisturisation on the forearm and face after 4 weeks of twice daily application compared to no treatment in participants with dry sensitive skin.

Publications & conference data

2 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Cosmetic benefit of a biomimetic lamellar cream formulation on barrier function or the appearance of fine lines and wrinkles in randomized proof-of-concept clinical studies.
    Nisbet S, Mahalingam H, Gfeller CF, Biggs E, et al · · 2019 · cited 5× · PMID 30414275 · DOI 10.1111/ics.12499
  2. Clinical Measurement of Transepidermal Water Loss.
    Kundu D, Jayaraman A, Sen CK. · · 2026 · cited 4× · PMID 40476522 · DOI 10.1089/wound.2024.0148

Verify or expand the search:

Other trials of Test product (Moisturising cream)

Trials testing the same drug.

Other GlaxoSmithKline trials

Trials by the same sponsor.

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Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT03216265.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing