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NCT03155269

To Investigate the Impact of a Nutritional Supplement on Bone Turnover Markers in Indian Healthy Premenopausal Women (25- 45 Years) After 6 Months of Intervention

Completed NA Results posted Last updated 12 April 2019
What this trial tests

NA trial testing Fortified Beverage Powder in Growth and Development in 114 participants. Completed in 16 January 2018.

Timeline
25 May 2017
Primary endpoint
16 January 2018
16 January 2018

Quick facts

Lead sponsorGlaxoSmithKline
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationrandomized
Designparallel
Maskingtriple
Primary purposeprevention
Enrollment114
Start date25 May 2017
Primary completion16 January 2018
Estimated completion16 January 2018
Sites1 location across India

Drugs / interventions tested

Conditions studied

Sponsor

GlaxoSmithKline — full company profile →

Who can join

Adults 25 to 45, female only, with Growth and Development. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Change From Baseline in Serum Cross Linking C-telopeptide of Type 1 Collagen (s-CTX-1) at 6 Months Primary · At baseline and at 6 months

s-CTX-1 is a bone resorption marker which is used to assess the bone health. After 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. CTX-1 serum was analysed using biochemical tests from the samples stored. Decreased s-CTX-1 is associated with improved bone health.

At Baseline
GroupValue95% CI
Test Product0.4440± 0.11932
Reference Product0.4410± 0.16213
At 6 months
GroupValue95% CI
Test Product0.2828± 0.14218
Reference Product0.3245± 0.16755
Change from baseline at 6 months
GroupValue95% CI
Test Product-0.1612± 0.15011
Reference Product-0.1165± 0.16574
Change From Baseline in the Ratio of Carboxylated (c-OC) to Under-carboxylated Osteocalcin (Uc-OC) at 6 Months Primary · At baseline and 6 months

c-OC/ uc-OC is considered as a surrogate marker of bone formation which is used to assess the bone health. After 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. c-OC/ uc-OC levels were analysed using biochemical tests from the samples stored. Increased c-OC/ uc-OC is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product1.208± 0.7573
Reference Product1.138± 0.6747
At 6 months
GroupValue95% CI
Test Product1.492± 0.6666
Reference Product1.251± 0.4858
Change from baseline at 6 months
GroupValue95% CI
Test Product0.284± 0.8961
Reference Product0.113± 0.7160
Change From Baseline in Serum Cross Linking C-telopeptide of Type 1 Collagen (s-CTX-1) at 3 Months Secondary · At baseline and at 3 months

s-CTX-1 is a bone resorption marker which is used to assess the bone health. After 3 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. CTX-1 serum was analysed using biochemical tests from the samples stored. Decreased s-CTX-1 is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product0.4440± 0.11932
Reference Product0.4410± 0.16213
At 3 months
GroupValue95% CI
Test Product0.2996± 0.15762
Reference Product0.3302± 0.16693
Change from baseline at 3 months
GroupValue95% CI
Test Product-0.1444± 0.14563
Reference Product-0.1108± 0.13265
Change From Baseline in the Ratio of Carboxylated (c-OC) to Under-carboxylated Osteocalcin (Uc-OC) at 3 Months Secondary · At baseline and at 3 months

c-OC/ uc-OC is considered as a surrogate marker of bone formation which is used to assess the bone health. After 3 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. c-OC/ uc-OC levels were analysed using biochemical tests from the samples stored. Increased c-OC/ uc-OC is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product1.208± 0.7573
Reference Product1.138± 0.6747
At 3 months
GroupValue95% CI
Test Product1.706± 1.2083
Reference Product1.192± 0.8305
Change from baseline at 3 months
GroupValue95% CI
Test Product0.498± 1.3043
Reference Product0.054± 0.8868
Change From Baseline in Urinary Cross Linking C-telopeptide of Type 1 Collagen at 3 Months and 6 Months Secondary · At baseline, at 3 and 6 months

Urinary-CTX-1 is a bone resorption surrogate marker which is used to assess the bone health. After 3 months and 6 months of taking the allocated product, spot urinary sample were collected. CTX-1 urine levels were analysed using biochemical tests from the samples stored. Decreased urinary CTX-1 is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product23.153± 17.3511
Reference Product25.137± 22.8524
At 3 months
GroupValue95% CI
Test Product21.105± 16.8386
Reference Product31.864± 27.5339
Change from baseline at 3 months
GroupValue95% CI
Test Product-1.309± 20.8967
Reference Product6.727± 22.7372
At 6 months
GroupValue95% CI
Test Product16.318± 17.1383
Reference Product18.708± 20.6598
Change from baseline at 6 months
GroupValue95% CI
Test Product-6.835± 22.1252
Reference Product-6.429± 27.9624
Change From Baseline in Serum N-terminal Telopeptide of Type 1 Collagen (s-NTX-1) at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

NTX-1 is a bone resorption surrogate marker which is used to assess the bone health. After 3 months and 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. NTX-1 serum was analysed using biochemical tests from the samples stored. Decreased s-NTX-1 is associated with improved bone health. The unit of measurement is nanomole bone collagen equivalent (NM BCE).

At baseline
GroupValue95% CI
Test Product11.64± 3.373
Reference Product11.87± 3.093
At 3 months
GroupValue95% CI
Test Product10.68± 6.315
Reference Product10.00± 5.720
Change from baseline at 3 months
GroupValue95% CI
Test Product-0.97± 6.466
Reference Product-1.99± 5.861
At 6 months
GroupValue95% CI
Test Product8.22± 3.146
Reference Product9.38± 4.157
Change from baseline at 6 months
GroupValue95% CI
Test Product-3.43± 4.072
Reference Product-2.49± 4.296
Change From Baseline in Serum Procollagen Type 1 N-terminal Propeptide (s-P1NP) at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

P1NP is a bone formation surrogate marker which is used to assess the bone health being the most abundant protein of bone matrix. After 3 months and 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. P1NP serum was analysed using biochemical tests from the samples stored. Increased s-P1NP is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product59.61± 22.044
Reference Product58.70± 23.133
At 3 months
GroupValue95% CI
Test Product46.64± 17.982
Reference Product54.22± 20.592
Change from baseline at 3 months
GroupValue95% CI
Test Product-12.96± 15.929
Reference Product-4.85± 16.498
At 6 months
GroupValue95% CI
Test Product43.86± 14.194
Reference Product51.12± 22.586
Change from baseline at 6 months
GroupValue95% CI
Test Product-15.75± 18.272
Reference Product-7.58± 18.037
Change From Baseline in Bone Specific Alkaline Phosphatase (BSAP) at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

BSAP is a bone formation surrogate marker which is used to assess the bone health. After 3 months and 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. BSAP serum was analysed using biochemical tests from the samples stored. Increased s-BSAP is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product11.071± 4.1076
Reference Product10.774± 3.0623
At 3 months
GroupValue95% CI
Test Product9.441± 4.3417
Reference Product10.188± 3.0810
Change from baseline at 3 months
GroupValue95% CI
Test Product-1.649± 1.8695
Reference Product-0.586± 1.7019
At 6 months
GroupValue95% CI
Test Product10.013± 3.3193
Reference Product11.754± 3.3887
Change from baseline at 6 months
GroupValue95% CI
Test Product-1.058± 4.1670
Reference Product0.980± 1.4648
Change From Baseline in Serum Parathyroid Hormone (s-PTH) at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

PTH is used to compare calcium concentration status which defines the healthy bones. Intact PTH is the biologically active form and is secreted when the calcium level is low. After 3 months and 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. PTH serum was analysed using biochemical tests from the samples stored. Decreased s-PTH is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product5.73± 2.875
Reference Product5.35± 2.499
At 3 months
GroupValue95% CI
Test Product4.99± 3.394
Reference Product5.75± 2.602
Change from baseline at 3 months
GroupValue95% CI
Test Product-0.66± 2.798
Reference Product0.34± 2.479
At 6 months
GroupValue95% CI
Test Product7.27± 3.429
Reference Product8.09± 4.303
Change from baseline at 6 months
GroupValue95% CI
Test Product1.53± 3.126
Reference Product2.69± 4.143
Change From Baseline in Urinary Calcium at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

Urinary calcium is used to compare calcium concentration status which defines the healthy bones. After 3 months and 6 months of taking the allocated product, spot urinary sample were collected. Urinary calcium was analysed using biochemical tests from the samples stored. Decreased urinary calcium is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product104.5± 78.26
Reference Product104.0± 82.26
At 3 months
GroupValue95% CI
Test Product161.5± 142.60
Reference Product116.9± 131.53
Change from baseline at 3 months
GroupValue95% CI
Test Product61.3± 126.75
Reference Product16.4± 98.00
At 6 months
GroupValue95% CI
Test Product338.3± 440.08
Reference Product549.7± 851.54
Change from baseline at 6 months
GroupValue95% CI
Test Product235.3± 451.33
Reference Product448.9± 846.48
Change From Baseline in Serum Calcium at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

Serum calcium is used to compare calcium concentration status which defines the healthy bones. After 3 months and 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. Serum calcium was analysed using biochemical tests from the samples stored. Increased serum calcium is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product2.299± 0.0629
Reference Product2.296± 0.0703
At 3 months
GroupValue95% CI
Test Product2.266± 0.0778
Reference Product2.258± 0.0862
Change from baseline at 3 months
GroupValue95% CI
Test Product-0.038± 0.0854
Reference Product-0.037± 0.0825
At 6 months
GroupValue95% CI
Test Product2.359± 0.0835
Reference Product2.326± 0.0792
Change from baseline at 6 months
GroupValue95% CI
Test Product0.060± 0.0873
Reference Product0.030± 0.0821
Change From Baseline in Serum Phosphorus at 3 Months and 6 Months Secondary · At baseline, at 3 months and 6 months

Serum phosphorus is a diagnostic marker for assessment of healthy bones. After 3 months and 6 months of taking the allocated product, blood sample was collected under 12-hour fasting condition. Blood serum of whole blood collected from each participant was then isolated by the method of centrifugation. Serum phosphorus was analysed using biochemical tests from the samples stored. Increased serum phosphorus is associated with improved bone health.

At baseline
GroupValue95% CI
Test Product1.138± 0.1286
Reference Product1.127± 0.1378
At 3 months
GroupValue95% CI
Test Product1.157± 0.1879
Reference Product1.184± 0.1621
Change from baseline at 3 months
GroupValue95% CI
Test Product0.023± 0.1832
Reference Product0.062± 0.1580
At 6 months
GroupValue95% CI
Test Product1.127± 0.1493
Reference Product1.123± 0.1810
Change from baseline at 6 months
GroupValue95% CI
Test Product-0.012± 0.1617
Reference Product-0.004± 0.1940

Adverse events — posted to ClinicalTrials.gov

Time frame: 180 days +/- 7. Reporting threshold: 1%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Test Product
Serious: 0/57 (0%)
Deaths: 0/57
Reference Product
Serious: 0/57 (0%)
Deaths: 0/57
Other adverse events (16 terms — click to expand)

ReactionSystemTest ProductReference Product
Blood PTH increasedInvestigations
Vitamin-D deficiencyMetabolism and nutrition disorders
Urine calcium/ creatinine ratio increasedInvestigations
NauseaGastrointestinal disorders
ConstipationGastrointestinal disorders
DyspepsiaGastrointestinal disorders
Abdominal PainGastrointestinal disorders
Abdominal DistensionGastrointestinal disorders
FlatulenceGastrointestinal disorders
Abdominal Pain UpperGastrointestinal disorders
DiarrhoeaGastrointestinal disorders
VomitingGastrointestinal disorders
Vitamin-B 12 deficiencyMetabolism and nutrition disorders
DysgeusiaNervous system disorders
HeadacheNervous system disorders
HungerGeneral disorders

Data from ClinicalTrials.gov NCT03155269 adverse events section.

Sponsor's own description

To investigate the effect of nutritional supplement on bone turnover markers (which are sensitive and respond quickly to nutrition or drug intervention) in Indian healthy premenopausal women after 6 months of intervention.

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

  1. Randomised Controlled Trial of Nutritional Supplement on Bone Turnover Markers in Indian Premenopausal Women.
    Umarji PB, Verma P, Garg V, Schini M, et al · · 2021 · cited 4× · PMID 33530298 · DOI 10.3390/nu13020364

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