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NCT03035331

Dendritic Cell Therapy, Cryosurgery, and Pembrolizumab in Treating Patients With Non-Hodgkin Lymphoma

Completed Phase 1, PHASE2 Results posted Last updated 16 May 2025
What this trial tests

Phase 1, PHASE2 trial testing Cryosurgery in Aggressive Non-Hodgkin Lymphoma in 11 participants. Completed in 10 September 2022.

Timeline
15 August 2017
Primary endpoint
25 January 2022
10 September 2022

Quick facts

Lead sponsorMayo Clinic
PhasePhase 1, PHASE2
StatusCompleted
Study typeINTERVENTIONAL
Allocationna
Designsingle group
Maskingnone
Primary purposetreatment
Enrollment11
Start date15 August 2017
Primary completion25 January 2022
Estimated completion10 September 2022
Sites1 location across United States

Drugs / interventions tested

Conditions studied

Sponsor

Mayo Clinic

Who can join

18 and older, any sex, with Aggressive Non-Hodgkin Lymphoma or Indolent Non-Hodgkin Lymphoma. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Number of Patients That Experienced a Dose Limiting Toxicity (DLT) Primary · 56 days

Maximum tolerated dose (MTD) will be defined as the dose level that does not induce dose limiting toxicity in at least one-third of patients. DLTs are defined as Grade 4 or 5 ANC or PLT for at least 7 days, grade 3 plus Infections and infestations, febrile neutropenia defined as fever ≥38.5oC (38 \>1 hour) with grade 4 plus neutropenia, at least Grade 3 erythema multiforme, ulceration, or urticaria that does not resolve to Grade 2 or less within \> three weeks, at least grade 3 bronchial obstruction, pneumonitis, or wheezing at least grade 3 allergic reaction or autoimmunity at least grade 3 t

GroupValue95% CI
Phase 1 Dose Level 10
Percentage of Complete Responses of Combination Therapy With Pembrolizumab, Cryoablation, and Intra-tumor Injection of Autologous Dendritic Cells (DC) at Maximum Tolerated Dose (MTD) Dose Primary · 24 months

The percentage of successes will be estimated by the number of successes divided by the total number of evaluable patients. Confidence intervals for the true success proportion will be calculated.

GroupValue95% CI
Phase 1 Dose Level 10
Phase 2 Dose Level 10
Progression Free Survival Secondary · 24 months

The distribution of survival time will be estimated using the method of Kaplan-Meier.

GroupValue95% CI
Phase 1 Dose Level 153.8 – NA
Phase 2 Dose Level 13.62.9 – NA
Treatment Free Survival Secondary · 34 months

The distribution of treatment-free survival will be estimated using the method of Kaplan-Meier.

GroupValue95% CI
Phase 1 Dose Level 115.8NA – NA
Phase 2 Dose Level 118.15.2 – NA
Duration of Response Secondary · 11 months

The distribution of duration of complete response will be estimated using the method of Kaplan-Meier.

GroupValue95% CI
All Patients61.4 – NA
Disease Free Survival Rate Secondary · 2 years

The percentage of patients without disease at two years post start of treatment.

GroupValue95% CI
Phase 1 Dose Level 10
Phase 2 Dose Level 10
Overall Survival Secondary · 5 years

The distribution of survival time will be estimated using the method of Kaplan-Meier.

GroupValue95% CI
Phase 1 Dose Level 1NA3.8 – NA
Phase 2 Dose Level 1NA3.6 – NA
Percentage of Patients With Grade 4 or 5 Adverse Events Secondary · Up to 4 years

Will be assessed by Common Terminology Criteria for Adverse Events version 4.0.

GroupValue95% CI
Phase 1 Dose Level 10
Phase 2 Dose Level 10
Percent of Patients With Improved Quality of Life Secondary · 4 years

Will be measured using the Functional Assessment of Cancer Therapy-lymphoma. The assessment will be scored according to the scoring algorithm. Changes from baseline will be calculated at each assessment time points. Mean change scores at each time point will be calculated to determine if quality of life is reduced over the course of treatment. Longitudinal techniques will be employed to describe changes over time.

GroupValue95% CI
Phase 1 Dose Level 133.3
Phase 2 Dose Level 157.14

Adverse events — posted to ClinicalTrials.gov

Time frame: Adverse events time frame was 4 years, mortality time frame was 5 years.. Reporting threshold: 0%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Phase 1 Dose Level 1
Serious: 0/3 (0%)
Deaths: 1/3
Phase 2 Dose Level 1
Serious: 2/8 (25%)
Deaths: 4/8

Serious adverse events (2 terms)

ReactionSystemPhase 1 Dose Level 1Phase 2 Dose Level 1
Lung infectionInfections and infestations
Rash maculo-papularSkin and subcutaneous tissue disorders
Other adverse events (27 terms — click to expand)

ReactionSystemPhase 1 Dose Level 1Phase 2 Dose Level 1
AnemiaBlood and lymphatic system disorders
DiarrheaGastrointestinal disorders
Lymphocyte count decreasedInvestigations
Rash maculo-papularSkin and subcutaneous tissue disorders
NauseaGastrointestinal disorders
Platelet count decreasedInvestigations
HypothyroidismEndocrine disorders
VomitingGastrointestinal disorders
FatigueGeneral disorders
PainGeneral disorders
Neutrophil count decreasedInvestigations
CoughRespiratory, thoracic and mediastinal disorders
DyspneaRespiratory, thoracic and mediastinal disorders
ConstipationGastrointestinal disorders
Dry mouthGastrointestinal disorders
ChillsGeneral disorders
Infections and infestations - Oth specInfections and infestations
Lung infectionInfections and infestations
Urinary tract infectionInfections and infestations
Alanine aminotransferase increasedInvestigations
White blood cell decreasedInvestigations
HyponatremiaMetabolism and nutrition disorders
Back painMusculoskeletal and connective tissue disorders
HeadacheNervous system disorders
ParesthesiaNervous system disorders
DepressionPsychiatric disorders
PruritusSkin and subcutaneous tissue disorders

Most-reported serious reactions: Lung infection, Rash maculo-papular.

Data from ClinicalTrials.gov NCT03035331 adverse events section.

Sponsor's own description

This phase I/II trial studies the best dose and side effects of dendritic cell therapy, cryosurgery and pembrolizumab in treating patients with non-Hodgkin lymphoma. Vaccines, such as dendritic cell therapy made from a person's tumor cells and white blood cells may help the body build an effective immune response to kill tumor cells. Cryosurgery kills cancer cells by freezing them. Immunotherapy with monoclonal antibodies, such as pembrolizumab, may help the body's immune system attack the cancer, and may interfere with the ability of tumor cells to grow and spread. Giving dendritic cell therapy, cryosurgery and pembrolizumab may work better at treating non-Hodgkin lymphoma.

Publications & conference data

8 peer-reviewed publications reference this trial (live from Europe PMC):

  1. Cancer vaccines as promising immuno-therapeutics: platforms and current progress.
    Liu J, Fu M, Wang M, Wan D, et al · · 2022 · cited 497× · PMID 35303904 · DOI 10.1186/s13045-022-01247-x
  2. Re-Emergence of Dendritic Cell Vaccines for Cancer Treatment.
    Saxena M, Bhardwaj N. · · 2018 · cited 231× · PMID 29458962 · DOI 10.1016/j.trecan.2017.12.007
  3. Research progress on dendritic cell vaccines in cancer immunotherapy.
    Yu J, Sun H, Cao W, Song Y, et al · · 2022 · cited 130× · PMID 35074008 · DOI 10.1186/s40164-022-00257-2
  4. Cryoablation and immunotherapy: an overview of evidence on its synergy.
    Aarts BM, Klompenhouwer EG, Rice SL, Imani F, et al · · 2019 · cited 108× · PMID 31111237 · DOI 10.1186/s13244-019-0727-5
  5. Towards superior dendritic-cell vaccines for cancer therapy.
    Saxena M, Balan S, Roudko V, Bhardwaj N. · · 2018 · cited 99× · PMID 30116654 · DOI 10.1038/s41551-018-0250-x
  6. Checkpoint blockade in Hodgkin and non-Hodgkin lymphoma.
    Merryman RW, Armand P, Wright KT, Rodig SJ. · · 2017 · cited 87× · PMID 29296917 · DOI 10.1182/bloodadvances.2017012534
  7. Dendritic Cell Vaccines: A Shift from Conventional Approach to New Generations.
    Lee KW, Yam JWP, Mao X. · · 2023 · cited 75× · PMID 37681880 · DOI 10.3390/cells12172147
  8. Dendritic Cells and Programmed Death-1 Blockade: A Joint Venture to Combat Cancer.
    Versteven M, Van den Bergh JMJ, Marcq E, Smits ELJ, et al · · 2018 · cited 75× · PMID 29599770 · DOI 10.3389/fimmu.2018.00394

Verify or expand the search:

Other trials of Cryosurgery

Trials testing the same drug.

Other recruiting trials for Aggressive Non-Hodgkin Lymphoma

Currently open trials in the same condition.

Other Mayo Clinic trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT03035331.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing