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NCT02572388

A Study to Assess the Safety and Immunogenicity of the Malaria Vaccine, R21, Administered With and Without Matrix-M1

Completed Phase 1 Results posted Last updated 12 November 2019
What this trial tests

Phase 1 trial testing R21 in Malaria in 31 participants. Completed in 29 August 2017.

Timeline
15 October 2015
Primary endpoint
29 August 2017
29 August 2017

Quick facts

Lead sponsorUniversity of Oxford
PhasePhase 1
StatusCompleted
Study typeINTERVENTIONAL
Allocationnon randomized
Designparallel
Maskingnone
Primary purposeprevention
Enrollment31
Start date15 October 2015
Primary completion29 August 2017
Estimated completion29 August 2017
Sites2 locations across United Kingdom

Drugs / interventions tested

Conditions studied

Sponsor

University of Oxford

Who can join

Adults 18 to 50, any sex, with Malaria. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Safety and Tolerability of Administration of R21 With and Without the Adjuvant Matrix-M1 Assessed by the Occurrence of Solicited Local and Systemic Adverse Events. Primary · Assessment of solicited AEs in the first 7 days post vaccination.

Occurrence of solicited local and systemic adverse events (i.e: pain, redness, swelling and pruritus at injection site and temperature, feverishness, myalgia, arthralgia, malaise, headache and nausea).

GroupValue95% CI
Group 1103
Group 221
Group 394
Group 421
Safety and Tolerability of R21 With and Without the Adjuvant Matrix-M1 Assessed by the Occurrence of Unsolicited Adverse Events. Primary · Unsolicited AEs to be assessed up to 28 days post vaccination.

Occurrence of unsolicited local and systemic adverse events.

GroupValue95% CI
Group 111
Group 24
Group 310
Group 46
Safety and Tolerability of R21 With and Without the Adjuvant Matrix-M1 Assessed by the Occurrence of Serious Adverse Events. Primary · 6 months

Occurrence of serious adverse events collected from enrolment until the end of the follow-up period.

GroupValue95% CI
Group 11
Group 20
Group 30
Group 41
Safety and Tolerability of R21 With and Without the Adjuvant Matrix-M1 Assessed by the Occurrence of Laboratory Adverse Events. Primary · At Day 0 (baseline), day 7 and day 28 post vaccination

Occurrence of laboratory adverse events defined as clinically significant changes from baseline. Haematology (Full Blood Count) and Biochemistry (Kidney and Liver Function Tests) will be assessed.

GroupValue95% CI
Group 111
Group 24
Group 310
Group 46

Adverse events — posted to ClinicalTrials.gov

Time frame: Solicited adverse events will be recorded daily for 7 days post-vaccination Unsolicited AEs of all severities will be recorded from receipt of vaccination through 28 days post-vaccination. After study Day 28, only SAEs or new chronic medical conditions that require ongoing medical management will be recorded through to the last study visit.. Reporting threshold: 5%. Adverse-event reports describe events observed during the trial — not all are caused by the drug.

Group 1
Serious: 1/11 (9%)
Deaths: 0/11
Group 2
Serious: 0/4 (0%)
Deaths: 0/4
Group 3
Serious: 0/10 (0%)
Deaths: 0/10
Group 4
Serious: 1/6 (17%)
Deaths: 0/6

Serious adverse events (2 terms)

ReactionSystemGroup 1Group 2Group 3Group 4
Bartholin's cystReproductive system and breast disorders
Rheumatoid arthritisMusculoskeletal and connective tissue disorders
Other adverse events (40 terms — click to expand)

ReactionSystemGroup 1Group 2Group 3Group 4
PainGeneral disorders
PainGeneral disorders
PainGeneral disorders
RednessSkin and subcutaneous tissue disorders
ArthralgiaMusculoskeletal and connective tissue disorders
FatigueGeneral disorders
HeadacheGeneral disorders
ArthralgiaMusculoskeletal and connective tissue disorders
FatigueGeneral disorders
HeadacheGeneral disorders
FatigueGeneral disorders
HeadacheGeneral disorders
WarmthSkin and subcutaneous tissue disorders
MalaiseGeneral disorders
RednessSkin and subcutaneous tissue disorders
WarmthSkin and subcutaneous tissue disorders
ArthralgiaMusculoskeletal and connective tissue disorders
FeverishnessGeneral disorders
NauseaGeneral disorders
MalaiseGeneral disorders
ItchSkin and subcutaneous tissue disorders
WarmthSkin and subcutaneous tissue disorders
RednessSkin and subcutaneous tissue disorders
ItchSkin and subcutaneous tissue disorders
MyalgiaMusculoskeletal and connective tissue disorders
NauseaGeneral disorders
ItchSkin and subcutaneous tissue disorders
FeverGeneral disorders
FeverishnessGeneral disorders
MyalgiaMusculoskeletal and connective tissue disorders
NauseaGeneral disorders
MalaiseGeneral disorders
AnaemiaBlood and lymphatic system disorders
SwellingSkin and subcutaneous tissue disorders
FeverishnessGeneral disorders
SwellingSkin and subcutaneous tissue disorders
MyalgiaMusculoskeletal and connective tissue disorders
SwellingSkin and subcutaneous tissue disorders
AnaemiaBlood and lymphatic system disorders
AnaemiaBlood and lymphatic system disorders

Most-reported serious reactions: Bartholin's cyst, Rheumatoid arthritis.

Data from ClinicalTrials.gov NCT02572388 adverse events section.

Sponsor's own description

This is a clinical trial in which healthy volunteers will be administered one or two experimental malaria vaccines. The vaccine R21 will either be administered alone or in combination with the adjuvant vaccine Matrix-M1. All vaccinations will be administered intramuscularly. Each volunteer will receive three vaccinations in total. There are three different vaccine schedules: Group 1 will receive 10µg of R21 mixed with 50µg of Matrix-M1 on days 0, 28, and 56. Group 2 will receive 50µg of R21 on days 0, 28, and 56. . Group 3 will receive 50µg of R21 mixed with 50µg of Matrix-M1 on days 0, 28, and 56. The study will assess the safety of the vaccines, and the immune responses to the vaccinations. Immune responses are measured by tests on blood samples. Healthy adult volunteers will be recruited in Oxford and London, England.

Publications & conference data

7 peer-reviewed publications reference this trial (live from Europe PMC):

  1. The Matrix-M™ adjuvant: A critical component of vaccines for the 21<sup>st</sup> century.
    Stertman L, Palm AE, Zarnegar B, Carow B, et al · · 2023 · cited 72× · PMID 37113023 · DOI 10.1080/21645515.2023.2189885
  2. Accelerating the clinical development of protein-based vaccines for malaria by efficient purification using a four amino acid C-terminal 'C-tag'.
    Jin J, Hjerrild KA, Silk SE, Brown RE, et al · · 2017 · cited 52× · PMID 28153778 · DOI 10.1016/j.ijpara.2016.12.001
  3. Novel Strategies for Malaria Vaccine Design.
    Frimpong A, Kusi KA, Ofori MF, Ndifon W. · · 2018 · cited 41× · PMID 30555463 · DOI 10.3389/fimmu.2018.02769
  4. Repertoire, function, and structure of serological antibodies induced by the R21/Matrix-M malaria vaccine.
    McDaniel JR, Voss WN, Bowyer G, Rush SA, et al · · 2025 · cited 5× · PMID 40719751 · DOI 10.1084/jem.20241908
  5. Evaluation of a novel malaria anti-sporozoite vaccine candidate, R21 in Matrix-M adjuvant, in the UK and Burkina Faso: two phase 1, first-in-human trials.
    Venkatraman N, Tiono AB, Bowyer G, Bellamy DG, et al · · 2025 · cited 5× · PMID 39805302 · DOI 10.1016/s2666-5247(24)00084-3
  6. Bioresponsive engineered nanoparticles for immunomodulation.
    Hegde M, Mishra A, Banerjee R, Bintee B, et al · · 2025 · cited 4× · PMID 41162977 · DOI 10.1186/s12916-025-04305-6
  7. Ethics & utility of controlled human infection studies (CHIS) in low- & middle-income countries.
    Eberts JD, Eyal N, Banerjee S. · · 2024 · cited 1× · PMID 39632640 · DOI 10.25259/ijmr_985_2024

Verify or expand the search:

Other trials of R21

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Trials by the same sponsor.

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Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing