Last reviewed · How we verify

NCT01716533

Evaluation of the Immune Response to Clostridium Difficile in Adults With Clostridium Difficile Infection (CDI)

Completed NA Results posted Last updated 7 June 2019
What this trial tests

NA trial testing Blood sampling in Infections, Clostridium Difficile in 57 participants. Completed in 1 June 2015.

Timeline
2 February 2013
Primary endpoint
1 June 2015
1 June 2015

Quick facts

Lead sponsorGlaxoSmithKline
PhaseNA
StatusCompleted
Study typeINTERVENTIONAL
Allocationnon randomized
Designparallel
Maskingnone
Primary purposebasic science
Enrollment57
Start date2 February 2013
Primary completion1 June 2015
Estimated completion1 June 2015
Sites5 locations across Canada, United States

Drugs / interventions tested

Conditions studied

Sponsor

GlaxoSmithKline — full company profile →

Who can join

18 and older, any sex, with Infections, Clostridium Difficile. Patients with the condition only — healthy volunteers not accepted.

Results — posted to ClinicalTrials.gov

Per-arm endpoint measurements with 95% confidence intervals where reported. Source: trial results section.

Serum Anti-toxin A and Anti-toxin B Antibody Concentrations at Day 14 Primary · At Day 14

Serum anti-toxin B antibody concentrations were expressed as Geometric Mean Concentrations (GMCs), as measured by the Enzyme Linked Immunosorbent Assay (ELISA) for the cut-off of equal to or above (≥) 100 EU/mL.

GroupValue95% CI
Recurrence Group2083.3995.8 – 4358.5
Sustained Response Group3189.41338.4 – 7600.2
Unclassified Group32096.426.1 – 39518495
Serum F2 C-terminal Anti-toxin B Antibody Concentrations Primary · At Day 14

Serum F2 C-terminal anti-toxin B antibody concentrations were expressed as Geometric Mean Concentrations (GMCs), as measured by ELISA for the cut-off of 13.16 EU/mL.

GroupValue95% CI
Recurrence Group149.320.2 – 1102.9
Sustained Response Group246.652.7 – 1154.8
Unclassified Group10736.71.7 – 69276208
Serum Anti-toxin A and Anti-toxin B Antibody Concentrations at Day 0 and Day 72 Secondary · At Day 0 and at Day 72

Serum anti-toxin B antibody concentrations were expressed as Geometric Mean Concentrations (GMCs), as measured by ELISA for the cut-off equal to or above (≥) 100 EU/mL.

Anti-toxin B at Day 0
GroupValue95% CI
Recurrence Group1939.6847.2 – 4440.6
Sustained Response Group1682.9858.7 – 3298.2
Unclassified Group19638.5217.5 – 1773406
Anti-toxin B at Day 72
GroupValue95% CI
Recurrence Group4471.11441.1 – 13871.8
Sustained Response Group3670.41519.8 – 8864.6
Unclassified Group64050.6NA – NA
Serum Neutralizing Anti-toxin A and Anti-toxin B Antibody Titers at Day 14 Secondary · At Day 14

Neutralizing antibody concentrations were expressed as Geometric Mean Titers (GMTs), as measured in an inhibition of cytotoxicity assay (toxin neutralization assays) for the cut-off of 2, expressed in 1/DIL unit, in which DIL is the sample dilution corresponding to 50% neutralization.

Neutralizing anti-toxin A
GroupValue95% CI
Recurrence Group11.30.7 – 194.8
Sustained Response Group2.31.2 – 4.5
Unclassified Group4.70.0 – 1584000000
Neutralizing anti-toxin B
GroupValue95% CI
Recurrence Group2.70.4 – 18.2
Sustained Response Group15.34.2 – 55.1
Unclassified Group1725.7125.5 – 23728.0
Serum Neutralizing Anti-toxin A and Anti-toxin B Antibody Titers at Day 0, Within 10 Days After Recurrence and at Day 72 Secondary · At Day 0, within 10 days after start of recurrent episode if any, and at end of follow-up (Day 72)

Neutralizing antibody concentrations were expressed as Geometric Mean Titers (GMTs), as measured in an inhibition of cytotoxicity assay (toxin neutralization assays) for the cut-off of 2, expressed in 1/DIL unit, in which DIL is the sample dilution corresponding to 50% neutralization. This measure concerned only diarrhea recurrence. No CDI recurrence was considered as part of the Group Sustained Response.

Neutralizing anti-toxin A, Day 0
GroupValue95% CI
Recurrence Group14.80.4 – 525.0
Sustained Response Group1.50.9 – 2.4
Unclassified Group3.20.0 – 7130211
Neutralizing anti-toxin A, recurrence 1
GroupValue95% CI
Recurrence Group12.20.6 – 241.2
Sustained Response Group1.0NA – NA
Neutralizing anti-toxin A, recurrence 2
GroupValue95% CI
Recurrence Group1.0NA – NA
Neutralizing anti-toxin A, Day 72
GroupValue95% CI
Recurrence Group11.10.3 – 422.0
Sustained Response Group2.51.2 – 5.4
Neutralizing anti-toxin B, Day 0
GroupValue95% CI
Recurrence Group3.30.4 – 28.0
Sustained Response Group9.62.7 – 34.1
Unclassified Group340.30.0 – 461890000
Neutralizing anti-toxin B, recurrence 1
GroupValue95% CI
Recurrence Group3.00.4 – 22.3
Sustained Response Group1.0NA – NA
Neutralizing anti-toxin B, recurrence 2
GroupValue95% CI
Recurrence Group1.0NA – NA
Neutralizing anti-toxin B, Day 72
GroupValue95% CI
Recurrence Group8.01.1 – 56.8
Sustained Response Group16.24.3 – 60.3
Unclassified Group1372.0NA – NA
Number of Subjects With Clostridium Difficile Infection (CDI) Recurrence Secondary · From Day 0 to Day 72

A CDI recurrence is defined as the development of a new episode of CDI following clinical response at the end of standard of care (SoC) for the initial CDI episode.

GroupValue95% CI
Recurrence Group7
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
CDI Initial Episodes Severity Characteristics, in All Subjects Secondary · At Day 0

Severity characteristics were expressed in duration of days for hospitalization, intensive care unit, Standard of Care (SoC) and CDI episodes.

Duration of hospitalization
GroupValue95% CI
Sustained Response Group5.04.0 – 11.0
Failure to Antibiotic Group7.07.0 – 7.0
Unclassified Group16.08.0 – 22.0
Duration in intensive care unit
GroupValue95% CI
Sustained Response Group5.04.0 – 6.0
Unclassified Group8.07.0 – 17.0
Duration of CDI episodes
GroupValue95% CI
Recurrence Group10.59.0 – 15.0
Sustained Response Group6.04.0 – 13.0
Failure to Antibiotic Group24.014.5 – 33.5
Unclassified Group10.05.0 – 15.0
Duration of SoC
GroupValue95% CI
Recurrence Group11.011.0 – 15.0
Sustained Response Group14.011.0 – 16.0
Failure to Antibiotic Group34.011.0 – 84.0
Unclassified Group11.010.0 – 26.0
Number of Subjects With Initial CDI Episode by Severity, in All Subjects Secondary · At Day 0

Initial CDI episodes were recorded by severity criteria: medical attention given, admission to intensive care unit, colectomy, death, high white blood cells (WBC) count, high creatinine count, hypotension/shock, clinical response to Standard of Care (SoC).

Medical attention - Medical contact without visit
GroupValue95% CI
Recurrence Group1
Sustained Response Group1
Failure to Antibiotic Group1
Unclassified Group0
Medical attention - Medical personnel visit
GroupValue95% CI
Recurrence Group2
Sustained Response Group3
Failure to Antibiotic Group1
Unclassified Group0
Medical attention - Emergency Room
GroupValue95% CI
Recurrence Group2
Sustained Response Group6
Failure to Antibiotic Group1
Unclassified Group1
Medical attention - Hospitalization
GroupValue95% CI
Recurrence Group2
Sustained Response Group15
Failure to Antibiotic Group2
Unclassified Group8
Intensive care unit - Yes
GroupValue95% CI
Recurrence Group0
Sustained Response Group3
Failure to Antibiotic Group0
Unclassified Group3
Intensive care unit - No
GroupValue95% CI
Recurrence Group7
Sustained Response Group22
Failure to Antibiotic Group5
Unclassified Group6
Colectomy - Yes
GroupValue95% CI
Recurrence Group0
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
Colectomy - No
GroupValue95% CI
Recurrence Group7
Sustained Response Group25
Failure to Antibiotic Group5
Unclassified Group9
Number of Subjects With CDI Recurrence by Severity, in Those Subjects Who Recur Secondary · At recurrence (within 10 days of start diarrhea) during a follow up period of up to 72 days per participant

A CDI recurrence is defined as the development of a new episode of CDI following clinical response at the end of standard of care (SoC) for the initial CDI episode. An episode of diarrhea was not considered as a recurrence of CDI if the stool was negative for C. difficile or if the diarrhea was attributed to another cause than C. difficile. The severity criteria for CDI recurrent episodes were categorized into non-severe and severe.

Non-severe
GroupValue95% CI
Recurrence Group7
Severe
GroupValue95% CI
Recurrence Group0
Number of Subjects With Failure of Antibiotic Treatment Secondary · Within 3 months before the initial CDI episodes

Failure of antibiotic treatment against C. difficile is defined as the persistence or the incomplete resolution of symptoms (more than one unformed stool per day) after a full course of antibiotic(s) therapy (minimum 7 days). Aminopen+BetaLactam Inhib,1st Gen.Cephalosporin = Aminopenicillin+Beta-Lactamase Inhibitor and 1st Generation Cephalosporin. Aminopen+BetaLactam Inhib, 3rd Gen.Cephalosporin = Aminopenicillin+Beta-Lactamase Inhibitor and 3rd Generation Cephalosporin excluding Ceftazidime and Fluoroquinolone. Aminopen+BetaLactam Inhib and Fluoroquinolone = Aminopenicillin+Beta-Lactamase

1st Generation Cephalosporin
GroupValue95% CI
Recurrence Group0
Sustained Response Group2
Failure to Antibiotic Group1
Unclassified Group0
1st Gen Cephalosporin+Tetracyclin, no Tigecyclin
GroupValue95% CI
Recurrence Group1
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
3rd Generation Cephalosporin, no Ceftazidime
GroupValue95% CI
Recurrence Group0
Sustained Response Group1
Failure to Antibiotic Group0
Unclassified Group0
4th Generation Cephalosporin and Fluoroquinolone
GroupValue95% CI
Recurrence Group0
Sustained Response Group1
Failure to Antibiotic Group0
Unclassified Group0
Aminopenicillin
GroupValue95% CI
Recurrence Group0
Sustained Response Group3
Failure to Antibiotic Group0
Unclassified Group0
Aminopen+BetaLactam Inhib,1st Gen.Cephalosporin
GroupValue95% CI
Recurrence Group1
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
Aminopen+BetaLactam Inhib, 3rd Gen.Cephalosporin
GroupValue95% CI
Recurrence Group1
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
Aminopen+BetaLactam Inhib and Fluoroquinolone
GroupValue95% CI
Recurrence Group0
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group1
Number of Subjects With Risk Factors Associated With the Initial CDI Episode Secondary · At Day 0

Risk factors for CDI included factors in three main domains involving host factors (advanced age, impaired immune status, co-morbidities); increased exposure to C. difficile spores (longer length of stays, healthcare environment, infected roommates or hand carriage by personnel); and factors that disrupt the normally protective colonic microflora layer (antimicrobials, other medications or procedures). CDI episodes within 6 months: Protocol exclusion criteria for enrolment allowed up to maximum 25% of the planned subjects having a previous CDI episode within the previous 6 months. Antibiotic

Setting of CDI development: Community
GroupValue95% CI
Recurrence Group5
Sustained Response Group18
Failure to Antibiotic Group3
Unclassified Group2
Setting of CDI development: Hospital
GroupValue95% CI
Recurrence Group2
Sustained Response Group6
Failure to Antibiotic Group1
Unclassified Group6
Setting of CDI development: Nursing home
GroupValue95% CI
Recurrence Group0
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
Setting of CDI development: Rehabilitation center
GroupValue95% CI
Recurrence Group0
Sustained Response Group0
Failure to Antibiotic Group0
Unclassified Group0
Setting of CDI development: Other
GroupValue95% CI
Recurrence Group0
Sustained Response Group1
Failure to Antibiotic Group1
Unclassified Group1
No CDI episodes within 6 months
GroupValue95% CI
Recurrence Group6
Sustained Response Group22
Failure to Antibiotic Group4
Unclassified Group8
CDI episodes within 6 months
GroupValue95% CI
Recurrence Group1
Sustained Response Group3
Failure to Antibiotic Group1
Unclassified Group1
No admission to hospital within 3 months
GroupValue95% CI
Recurrence Group6
Sustained Response Group15
Failure to Antibiotic Group4
Unclassified Group4
Number of Subjects With Risk Factors Associated With the CDI Recurrence Secondary · At recurrence (within 10 days of start diarrhea) during a follow up period of up to 72 days per participant

Risk factors for CDI included factors in three main domains involving host factors (advanced age, impaired immune status, co-morbidities); increased exposure to C. difficile spores (longer length of stays, healthcare environment, infected roommates or hand carriage by personnel); and factors that disrupt the normally protective colonic microflora layer (antimicrobials, other medications or procedures).

Setting of CDI development: Community
GroupValue95% CI
Recurrence Group6
Setting of CDI development: Hospital
GroupValue95% CI
Recurrence Group1
Setting of CDI development: Nursing home
GroupValue95% CI
Recurrence Group0
Setting of CDI development: Rehabilitation center
GroupValue95% CI
Recurrence Group0
Sustained Response Group0
Setting of CDI development: Other
GroupValue95% CI
Recurrence Group0
No admission to hospital during follow-up period
GroupValue95% CI
Recurrence Group7
Admission to hospital during follow-up period
GroupValue95% CI
Recurrence Group0
Not staying in nursing home during FU period
GroupValue95% CI
Recurrence Group7

Sponsor's own description

This study aims to 1) evaluate the C. difficile-specific immune response in CDI patients and 2) explore the difference in immune response between the patients with CDI recurrence and those with a sustained clinical response.

Publications & conference data

No peer-reviewed publications indexed yet for this trial. Completed trials usually publish results within 12-18 months.

Verify or expand the search:

Other trials of Blood sampling

Trials testing the same drug.

Other GlaxoSmithKline trials

Trials by the same sponsor.

Verify against primary sources

Data sources for this page

Drug Landscape aggregates and links these public records for informational use only. Always verify against the primary source before clinical or regulatory decisions. Canonical URL: https://druglandscape.com/trial/NCT01716533.

Primary sources · FDA · ClinicalTrials.gov · EMA · SEC EDGAR · ChEMBL · Wikidata · full sourcing