NCT00471250

Collection of Lung Fluid and Tissue Samples for Research

Recruiting now Last updated 16 January 2026

What this trial tests

trial in Leukocyte Disorders in 550 participants. Currently enrolling.

Timeline

17 August 2007

Quick facts

Lead sponsor	National Heart, Lung, and Blood Institute (NHLBI)
Status	Recruiting now
Study type	OBSERVATIONAL
Enrollment	550
Start date	17 August 2007
Sites	1 location across United States

Conditions studied

• Leukocyte Disorders — all drugs for Leukocyte Disorders →
• Respiratory Tract Diseases — all drugs for Respiratory Tract Diseases →
• Bronchoscopy — all drugs for Bronchoscopy →

Sponsor

National Heart, Lung, and Blood Institute (NHLBI)

Who can join

Adults 5 to 100, any sex, with Leukocyte Disorders or Respiratory Tract Diseases. Patients with the condition only — healthy volunteers not accepted.

Sponsor's own description

This study will collect fluid and tissue specimens from the lungs and nose of healthy people and people with a history of lung infections. The specimens will be examined for differences between the two groups that may be associated with susceptibility to certain infections.

Healthy normal volunteers and people with a history of lung infections between 18 and 75 years of age who are followed at NIH may be eligible for this study.

Participants undergo the following procedures:

Medical history and physical examination.

Blood and urine tests.

Electrocardiogram (ECG) and chest x-ray.

Treadmill exercise stress test (for people over 45 years old with a history of chest pain or ECG abnormalities).

Bronchoscopy: The subject s nose and throat are numbed with lidocaine and a sedative is given for comfort. A thin flexible tube called a bronchoscope is advanced through the nose or mouth into the lung airways to examine the airways carefully.

Fluid collection during the bronchoscopy using one of the following methods:

* Bronchoalveolar lavage: Salt water is injected through the bronchoscope into the lung and immediately suctioned out, washing off cells lining the airways.
* Bronchial brushings: A brush-tipped wire enclosed in a sheath is passed through the bronchoscope and a small area of the airway tissue is gently brushed. The brush is withdrawn with some tissue adhering to it.
* Endobronchial biopsies: Small pinchers on a wire are passed through the bronchoscope and about 1 to 2 millimeters of tissue is removed.
* Nasal scrape: A small device is used to scrape along the inside of the nose to collect some cells.
* Sputum induction
* Exhaled breath condensate to obtain specimens for in vitro investigations and comparisons of both the cellular and acellular components.
* Nasal nitric oxide production
* Nasal potential difference
* Exhaled aerosol mask sample collection
* Cough aerosol collection
* Exhaled particle collection
* Lung Clearance Index (LCI)

Publications & conference data

1 peer-reviewed publication reference this trial (live from Europe PMC):

1. Respiratory manifestations in 38 patients with Alström syndrome.
   Boerwinkle C, Marshall JD, Bryant J, Gahl WA, et al · · 2017 · cited 18× · PMID 28029746 · DOI 10.1002/ppul.23607