GB2602020A — A method for evaluating the pro- or anti convulsive properties of test compounds

USPTO Abstract

A method of assessing the pro- or anti-convulsive properties of compounds comprising: a. dosing a number of animals with either vehicle, test, or positive control compound, b. assessing the treated animal for production of tonic hind limb extensor convulsion at defined period of time post-dose from a single electroshock at a defined current, c. decreasing or increasing the defined current if the preceding animal did or did not show tonic hind limb extensor convulsion, respectively, c. collecting CC50 values for the animals, wherein that number of animals is at least 6. The number of animals may be no more than 11. The current may be changed in a logarithmic scale. The defined period of time post-dose may be at least 15, 30, or 120 minutes. The positive control may be diazepam, or valproate. The animal may be a mouse, rat, pig. Results are given showing the use of cannabidiol (CBD) analogues as the test compounds in mini-MEST (maximal electroshock seizure threshold) tests.