EP3849597A1 — Slow-cycling cell-rna based nanoparticle vaccine to treat cancer

USPTO Abstract

The present disclosure provides compositions comprising a liposome comprising a cationic lipid and nucleic acid molecules comprising a sequence of a nucleic acid molecule expressed by slow-cycling cells (SCCs). The present disclosure also provides methods of preparing an anti-tumor liposome composition. In exemplary embodiments, the method comprises (a) isolating SCCs from a mixed tumor cell population in accordance with any one of the presently disclosed in vitro method of isolating SCCs from a mixed tumor cell population, (b) extracting nucleic acid molecules from the isolated SCCs, and (c) mixing the nucleic acid molecules with a cationic lipid to make an anti-tumor liposome composition. The method of preparing an anti-tumor liposome composition in alternative embodiments comprises mixing at least one SCC transcriptome nucleic acid molecule as described herein with a cationic lipid to make an anti-tumor liposome composition. Tumor treatment methods are furthermore provided by the present disclosure.