CN107703111B — 一种梯度致密性荧光水凝胶及其制备方法和应用
Assigned to Lanzhou University · Expires 2020-06-16 · 6y expired
What this patent protects
本发明公开一种梯度致密性荧光水凝胶及其制备方法和应用。所述水凝胶包括A液及B液，A液：取0‑4重量份多聚甲醛、0.8重量份NaCl、0.3重量份Na 2 HPO 4 ·12H 2 O、0.02重量份KCl和0.02重量份KH 2 PO 4 ，去离子水定容至NaCl浓度为0.008g/ml后55℃溶解；冷却至4℃后调节pH至6.8‑7.6；加入0.025‑0.15重量份荧光微球、5‑15重量份丙烯酰胺、0.025‑0.075重量份N,N‑亚甲基二丙烯酰胺，4℃溶解后加入0.078重量份四甲基乙二胺和0.05‑0.2重量份过硫酸铵并震荡混匀。该水凝胶具有超强…
USPTO Abstract
本发明公开一种梯度致密性荧光水凝胶及其制备方法和应用。所述水凝胶包括A液及B液，A液：取0‑4重量份多聚甲醛、0.8重量份NaCl、0.3重量份Na 2 HPO 4 ·12H 2 O、0.02重量份KCl和0.02重量份KH 2 PO 4 ，去离子水定容至NaCl浓度为0.008g/ml后55℃溶解；冷却至4℃后调节pH至6.8‑7.6；加入0.025‑0.15重量份荧光微球、5‑15重量份丙烯酰胺、0.025‑0.075重量份N,N‑亚甲基二丙烯酰胺，4℃溶解后加入0.078重量份四甲基乙二胺和0.05‑0.2重量份过硫酸铵并震荡混匀。该水凝胶具有超强的耐组织透明处理性，可对大组织内的血管结构进行稳定荧光标记。
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