CN Patent

CN105441391A — 一种糖尿病并发抑郁症的细胞模型及建立方法和应用

Assigned to Hunan University of Chinese Medicine · Expires 2016-03-30 · 10y expired

What this patent protects

本发明属于生物技术领域，尤其涉及一种糖尿病并发抑郁症细胞模型的建立方法：向培养海马神经元的培养基中加入终浓度为50-200mmol/L葡萄糖和100-400μmol/L皮质酮，干预培养所述海马神经元18-24h，即得所述细胞模型。通过一般形态学观察、HE染色、葡萄糖消耗量检测、细胞内神经递质检测、MTT法检测、流式细胞仪技术、Hoechst荧光染色法和TUNEL染色法对所述模型进行了评价，显示该模型效果良好，可用于糖尿病并发抑郁症的病理生理研究或发病机制研究或体外药物筛选或药物评价。

USPTO Abstract

本发明属于生物技术领域，尤其涉及一种糖尿病并发抑郁症细胞模型的建立方法：向培养海马神经元的培养基中加入终浓度为50-200mmol/L葡萄糖和100-400μmol/L皮质酮，干预培养所述海马神经元18-24h，即得所述细胞模型。通过一般形态学观察、HE染色、葡萄糖消耗量检测、细胞内神经递质检测、MTT法检测、流式细胞仪技术、Hoechst荧光染色法和TUNEL染色法对所述模型进行了评价，显示该模型效果良好，可用于糖尿病并发抑郁症的病理生理研究或发病机制研究或体外药物筛选或药物评价。

Drugs covered by this patent

Patent Metadata

Patent number
CN105441391A
Jurisdiction
CN
Classification
—
Expires
2016-03-30
Drug substance claim
No
Drug product claim
No
Assignee
Hunan University of Chinese Medicine
Source
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Bibliographic data sourced from FDA Orange Book + USPTO public records. Plain-English summary generated by AI grounded in source text. Patent term extensions (PTR, SPC, pediatric) may shift the effective expiry. Not legal advice.

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