CN Patent

CN102153651B — 抗葡聚糖单链抗体及其制备方法

Assigned to Xiamen University · Expires 2012-11-14 · 14y expired

What this patent protects

抗葡聚糖单链抗体及其制备方法。涉及抗葡聚糖的抗体，提供抗葡聚糖单链抗体及其制备方法。克隆抗葡聚糖单链抗体基因，并将其导入载体，构建重组载体；将重组载体导入宿主细菌，构建表达抗葡聚糖单链抗体的重组工程菌；重组工程菌发酵培养，分离纯化发酵液，即得抗葡聚糖单链抗体。利用抗葡聚糖单克隆抗体杂交瘤细胞株D9，通过RT-PCR技术，获得了该抗体的重、轻链可变区序列，进一步组装成抗葡聚糖单链抗体基因，并构建重组表达载体pET-22(b+)-scFv，进行抗葡聚糖单链抗体的表达、纯化与鉴定。在基因工程技术的大背景下，抗葡聚糖单链抗体的获得将有助于未来该抗体的进一…

USPTO Abstract

抗葡聚糖单链抗体及其制备方法。涉及抗葡聚糖的抗体，提供抗葡聚糖单链抗体及其制备方法。克隆抗葡聚糖单链抗体基因，并将其导入载体，构建重组载体；将重组载体导入宿主细菌，构建表达抗葡聚糖单链抗体的重组工程菌；重组工程菌发酵培养，分离纯化发酵液，即得抗葡聚糖单链抗体。利用抗葡聚糖单克隆抗体杂交瘤细胞株D9，通过RT-PCR技术，获得了该抗体的重、轻链可变区序列，进一步组装成抗葡聚糖单链抗体基因，并构建重组表达载体pET-22(b+)-scFv，进行抗葡聚糖单链抗体的表达、纯化与鉴定。在基因工程技术的大背景下，抗葡聚糖单链抗体的获得将有助于未来该抗体的进一步开发和应用。

Drugs covered by this patent

Patent Metadata

Patent number
CN102153651B
Jurisdiction
CN
Classification
—
Expires
2012-11-14
Drug substance claim
No
Drug product claim
No
Assignee
Xiamen University
Source
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Bibliographic data sourced from FDA Orange Book + USPTO public records. Plain-English summary generated by AI grounded in source text. Patent term extensions (PTR, SPC, pediatric) may shift the effective expiry. Not legal advice.

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