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Placebo to Methotrexate

Amgen · Phase 3 active Small molecule

Methotrexate inhibits dihydrofolate reductase, blocking folate metabolism and reducing DNA synthesis and cell proliferation.

Methotrexate inhibits dihydrofolate reductase, blocking folate metabolism and reducing DNA synthesis and cell proliferation. Used for Rheumatoid arthritis, Various malignancies (acute leukemia, lymphoma, breast cancer), Psoriasis.

At a glance

Generic namePlacebo to Methotrexate
SponsorAmgen
Drug classAntimetabolite / Folate antagonist
TargetDihydrofolate reductase (DHFR)
ModalitySmall molecule
Therapeutic areaOncology / Immunology / Rheumatology
PhasePhase 3

Mechanism of action

Methotrexate is a folate antagonist that inhibits dihydrofolate reductase, an enzyme critical for converting dihydrofolate to tetrahydrofolate, which is essential for one-carbon transfer reactions in nucleotide synthesis. By depleting intracellular folate cofactors, it suppresses DNA and RNA synthesis, leading to reduced cell proliferation and increased apoptosis. At lower doses used in rheumatologic conditions, it also has immunomodulatory effects including reduced T-cell activation and cytokine production.

Approved indications

Common side effects

Key clinical trials

Primary sources

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SourceUsed for
ClinicalTrials.govTrial enrolment, design, endpoints, results

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