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Methotrexate - Immediate

University Hospital, Montpellier · Phase 3 active Small molecule

Methotrexate inhibits dihydrofolate reductase, blocking the synthesis of purines and pyrimidines required for DNA replication and cell division.

Methotrexate inhibits dihydrofolate reductase, blocking the synthesis of purines and pyrimidines required for DNA replication and cell division. Used for Acute lymphoblastic leukemia (ALL), Osteosarcoma, Breast cancer.

At a glance

Generic nameMethotrexate - Immediate
Also known asconventional methotrexate treatment
SponsorUniversity Hospital, Montpellier
Drug classAntimetabolite; folate antagonist
TargetDihydrofolate reductase (DHFR)
ModalitySmall molecule
Therapeutic areaOncology; Immunology; Rheumatology
PhasePhase 3

Mechanism of action

Methotrexate is a folate antagonist that binds to dihydrofolate reductase and prevents the conversion of dihydrofolate to tetrahydrofolate, a critical cofactor in one-carbon transfer reactions. This disrupts nucleotide synthesis, leading to inhibition of rapidly dividing cells including cancer cells and activated immune cells. The drug is used both as a cytotoxic chemotherapy agent and as an immunosuppressant at lower doses.

Approved indications

Common side effects

Key clinical trials

Primary sources

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SourceUsed for
ClinicalTrials.govTrial enrolment, design, endpoints, results

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