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Intravitreal Methotrexate 400 µg

JHSPH Center for Clinical Trials · Phase 3 active Small molecule

Intravitreal methotrexate is a chemotherapy agent injected directly into the eye that inhibits dihydrofolate reductase, suppressing DNA synthesis and reducing proliferation of malignant cells in ocular lymphomas.

Intravitreal methotrexate is a chemotherapy agent injected directly into the eye that inhibits dihydrofolate reductase, suppressing DNA synthesis and reducing proliferation of malignant cells in ocular lymphomas. Used for Primary central nervous system lymphoma with vitreous involvement, Intraocular lymphoma.

At a glance

Generic nameIntravitreal Methotrexate 400 µg
SponsorJHSPH Center for Clinical Trials
Drug classAntimetabolite; Folate antagonist
TargetDihydrofolate reductase (DHFR)
ModalitySmall molecule
Therapeutic areaOncology
PhasePhase 3

Mechanism of action

Methotrexate is a folate antagonist that blocks dihydrofolate reductase, preventing the conversion of dihydrofolate to tetrahydrofolate, which is essential for one-carbon transfer reactions and DNA/RNA synthesis. Direct intravitreal administration delivers high local concentrations to the vitreous cavity while minimizing systemic exposure, making it suitable for treating intraocular lymphomas and other vitreous malignancies. The drug's antiproliferative effects target rapidly dividing lymphoid cells.

Approved indications

Common side effects

Key clinical trials

Primary sources

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SourceUsed for
ClinicalTrials.govTrial enrolment, design, endpoints, results

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