Last reviewed 2026-04-22 · How we verify

ChloraPrep One-Step

ChloraPrep One-Step is a topical antiseptic that kills microorganisms on the skin through the antimicrobial action of chlorhexidine gluconate and isopropyl alcohol.

ChloraPrep One-Step is a topical antiseptic that kills microorganisms on the skin through the antimicrobial action of chlorhexidine gluconate and isopropyl alcohol. Used for Skin antisepsis prior to surgery or invasive procedures, Preparation of skin for injection sites and catheter insertion.

At a glance

Mechanism of action

The product combines chlorhexidine gluconate (a broad-spectrum antimicrobial agent) with isopropyl alcohol to provide rapid and sustained skin antisepsis. Chlorhexidine disrupts bacterial cell membranes and denatures proteins, while the alcohol component provides rapid microbicidal activity. Together, they reduce the microbial flora on the skin to prevent surgical site infections and other procedure-related contamination.

Approved indications

• Skin antisepsis prior to surgery or invasive procedures
• Preparation of skin for injection sites and catheter insertion

Common side effects

• Skin irritation or erythema
• Contact dermatitis
• Allergic reaction (rare)

Key clinical trials

• Trigger Point Injections Versus Lidocaine Patch for Myofascial Pain in the Emergency Department (PHASE4)
• Aqueous vs Alcohol Chlorhexidine Skin Preparation for Prevention of Cesarean Section Wound Infections (PHASE4)
• Topical Antimicrobial Effectiveness Testing (PHASE4)
• Topical Antimicrobial Effectiveness Testing (PHASE4)
• Test for Pre Operative Skin Preparations (PHASE3)
• Pilot Evaluation of the Application Procedure on the Antimicrobial Effects of 2 Antiseptics (PHASE3)
• Preoperative Skin Preparation Evaluation (PHASE3)
• Comparison of Alcoholic Chlorhexidine 2% Versus Alcoholic Povidone Iodine for Infections Prevention With Cardiac Resynchronization Therapy Device Implantation (PHASE4)

Primary sources

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